Experiment: Mannan from Saccharomyces cerevisiae (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3314 and BT3315 are separated by 263 nucleotides BT3315 and BT3316 are separated by 11 nucleotides BT3316 and BT3317 are separated by 507 nucleotides
BT3314: BT3314 - thermostable beta-glucosidase B (NCBI ptt file), at 4,256,693 to 4,258,987
BT3314
BT3315: BT3315 - hypothetical protein (NCBI ptt file), at 4,259,251 to 4,259,442
BT3315
BT3316: BT3316 - hypothetical protein (NCBI ptt file), at 4,259,454 to 4,259,906
BT3316
BT3317: BT3317 - conserved hypothetical protein (NCBI ptt file), at 4,260,414 to 4,261,043
BT3317
Position (kb)
4259
4260 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4258.263 kb on + strand, within BT3314 at 4258.285 kb on - strand, within BT3314 at 4258.285 kb on - strand, within BT3314 at 4258.489 kb on + strand, within BT3314 at 4258.530 kb on + strand, within BT3314 at 4258.531 kb on - strand, within BT3314 at 4258.531 kb on - strand, within BT3314 at 4258.627 kb on + strand, within BT3314 at 4258.628 kb on - strand, within BT3314 at 4258.630 kb on + strand, within BT3314 at 4258.684 kb on + strand, within BT3314 at 4258.684 kb on + strand, within BT3314 at 4258.714 kb on + strand, within BT3314 at 4258.720 kb on + strand, within BT3314 at 4258.720 kb on + strand, within BT3314 at 4258.742 kb on - strand, within BT3314 at 4258.969 kb on - strand at 4258.985 kb on + strand at 4258.986 kb on - strand at 4259.049 kb on + strand at 4259.054 kb on + strand at 4259.055 kb on - strand at 4259.055 kb on - strand at 4259.112 kb on + strand at 4259.113 kb on - strand at 4259.128 kb on - strand at 4259.128 kb on - strand at 4259.128 kb on - strand at 4259.147 kb on + strand at 4259.147 kb on + strand at 4259.147 kb on + strand at 4259.148 kb on - strand at 4259.148 kb on - strand at 4259.159 kb on - strand at 4259.189 kb on - strand at 4259.196 kb on - strand at 4259.249 kb on - strand at 4259.268 kb on - strand at 4259.268 kb on - strand at 4259.287 kb on + strand, within BT3315 at 4259.288 kb on - strand, within BT3315 at 4259.288 kb on - strand, within BT3315 at 4259.313 kb on + strand, within BT3315 at 4259.322 kb on - strand, within BT3315 at 4259.384 kb on + strand, within BT3315 at 4259.385 kb on - strand, within BT3315 at 4259.391 kb on + strand, within BT3315 at 4259.425 kb on - strand at 4259.445 kb on + strand at 4259.446 kb on - strand at 4259.454 kb on - strand at 4259.554 kb on - strand, within BT3316 at 4259.559 kb on - strand, within BT3316 at 4259.708 kb on + strand at 4259.708 kb on + strand, within BT3316 at 4259.708 kb on + strand, within BT3316 at 4259.727 kb on - strand, within BT3316 at 4259.736 kb on - strand, within BT3316 at 4259.775 kb on - strand, within BT3316 at 4259.777 kb on - strand, within BT3316 at 4259.777 kb on - strand, within BT3316 at 4259.777 kb on - strand, within BT3316 at 4259.791 kb on + strand, within BT3316 at 4259.798 kb on + strand, within BT3316 at 4259.810 kb on + strand, within BT3316 at 4259.842 kb on - strand, within BT3316 at 4259.857 kb on + strand, within BT3316 at 4259.858 kb on - strand, within BT3316 at 4259.922 kb on - strand at 4259.942 kb on - strand at 4259.957 kb on - strand at 4259.965 kb on - strand at 4260.006 kb on - strand at 4260.158 kb on - strand at 4260.182 kb on + strand at 4260.182 kb on + strand at 4260.191 kb on - strand at 4260.222 kb on + strand at 4260.240 kb on + strand at 4260.240 kb on + strand at 4260.241 kb on - strand at 4260.263 kb on - strand at 4260.263 kb on - strand at 4260.285 kb on - strand at 4260.285 kb on - strand at 4260.356 kb on + strand at 4260.358 kb on + strand at 4260.358 kb on + strand at 4260.359 kb on - strand at 4260.359 kb on - strand at 4260.363 kb on + strand at 4260.364 kb on - strand at 4260.368 kb on + strand at 4260.368 kb on + strand at 4260.369 kb on - strand at 4260.369 kb on - strand at 4260.407 kb on + strand at 4260.407 kb on + strand at 4260.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae (C) remove 4,258,263 + BT3314 0.68 +0.1 4,258,285 - BT3314 0.69 -1.1 4,258,285 - BT3314 0.69 -0.8 4,258,489 + BT3314 0.78 -2.2 4,258,530 + BT3314 0.80 -4.6 4,258,531 - BT3314 0.80 -3.0 4,258,531 - BT3314 0.80 -2.0 4,258,627 + BT3314 0.84 -2.1 4,258,628 - BT3314 0.84 -1.4 4,258,630 + BT3314 0.84 -2.7 4,258,684 + BT3314 0.87 -0.3 4,258,684 + BT3314 0.87 -1.8 4,258,714 + BT3314 0.88 -3.8 4,258,720 + BT3314 0.88 -2.0 4,258,720 + BT3314 0.88 +0.6 4,258,742 - BT3314 0.89 +0.9 4,258,969 - -0.3 4,258,985 + -2.0 4,258,986 - -0.8 4,259,049 + -2.6 4,259,054 + +1.9 4,259,055 - -0.1 4,259,055 - -0.2 4,259,112 + +0.1 4,259,113 - -3.9 4,259,128 - -1.4 4,259,128 - -1.4 4,259,128 - -2.3 4,259,147 + +0.4 4,259,147 + -1.8 4,259,147 + -0.7 4,259,148 - -0.8 4,259,148 - -1.0 4,259,159 - -1.8 4,259,189 - -2.3 4,259,196 - -1.8 4,259,249 - +0.4 4,259,268 - -1.1 4,259,268 - -1.1 4,259,287 + BT3315 0.19 -0.5 4,259,288 - BT3315 0.19 +0.3 4,259,288 - BT3315 0.19 +0.4 4,259,313 + BT3315 0.32 -2.4 4,259,322 - BT3315 0.37 -1.4 4,259,384 + BT3315 0.69 -2.9 4,259,385 - BT3315 0.70 -3.3 4,259,391 + BT3315 0.73 -1.6 4,259,425 - +0.8 4,259,445 + -0.7 4,259,446 - -0.1 4,259,454 - -0.1 4,259,554 - BT3316 0.22 +0.9 4,259,559 - BT3316 0.23 -0.8 4,259,708 + +1.2 4,259,708 + BT3316 0.56 -0.1 4,259,708 + BT3316 0.56 +0.4 4,259,727 - BT3316 0.60 -0.4 4,259,736 - BT3316 0.62 -1.1 4,259,775 - BT3316 0.71 -2.1 4,259,777 - BT3316 0.71 +0.0 4,259,777 - BT3316 0.71 -2.2 4,259,777 - BT3316 0.71 -5.0 4,259,791 + BT3316 0.74 -1.1 4,259,798 + BT3316 0.76 +0.2 4,259,810 + BT3316 0.79 -1.1 4,259,842 - BT3316 0.86 -0.2 4,259,857 + BT3316 0.89 -1.6 4,259,858 - BT3316 0.89 +0.2 4,259,922 - +2.2 4,259,942 - -3.3 4,259,957 - +0.2 4,259,965 - -0.3 4,260,006 - +1.0 4,260,158 - -3.1 4,260,182 + -2.0 4,260,182 + -2.1 4,260,191 - -0.2 4,260,222 + +0.3 4,260,240 + +1.9 4,260,240 + -2.2 4,260,241 - -1.8 4,260,263 - -1.4 4,260,263 - +0.8 4,260,285 - -1.8 4,260,285 - -1.8 4,260,356 + -0.3 4,260,358 + -1.6 4,260,358 + +0.2 4,260,359 - +0.6 4,260,359 - -0.8 4,260,363 + -0.4 4,260,364 - -2.8 4,260,368 + -1.6 4,260,368 + +2.2 4,260,369 - -1.6 4,260,369 - -2.4 4,260,407 + +0.6 4,260,407 + -0.2 4,260,430 - -1.9
Or see this region's nucleotide sequence