Experiment: Mannan from Saccharomyces cerevisiae (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1932 and BT1933 are separated by 190 nucleotides BT1933 and BT1934 overlap by 54 nucleotides BT1934 and BT1935 are separated by 50 nucleotides
BT1932: BT1932 - hypothetical protein (NCBI ptt file), at 2,427,164 to 2,427,871
BT1932
BT1933: BT1933 - transposase (NCBI ptt file), at 2,428,062 to 2,428,334
BT1933
BT1934: BT1934 - hypothetical protein (NCBI ptt file), at 2,428,281 to 2,430,425
BT1934
BT1935: BT1935 - hypothetical protein (NCBI ptt file), at 2,430,476 to 2,432,677
BT1935
Position (kb)
2428
2429
2430
2431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2427.286 kb on - strand, within BT1932 at 2427.286 kb on - strand, within BT1932 at 2427.305 kb on + strand, within BT1932 at 2427.305 kb on + strand, within BT1932 at 2427.350 kb on + strand, within BT1932 at 2427.350 kb on + strand, within BT1932 at 2427.355 kb on + strand, within BT1932 at 2427.355 kb on + strand, within BT1932 at 2427.356 kb on - strand, within BT1932 at 2427.425 kb on - strand, within BT1932 at 2427.770 kb on + strand, within BT1932 at 2427.824 kb on - strand at 2427.968 kb on + strand at 2427.978 kb on - strand at 2427.987 kb on + strand at 2427.988 kb on - strand at 2428.043 kb on + strand at 2428.044 kb on - strand at 2428.044 kb on - strand at 2428.071 kb on + strand at 2428.072 kb on - strand at 2428.073 kb on + strand at 2428.074 kb on - strand at 2428.082 kb on + strand at 2428.142 kb on - strand, within BT1933 at 2428.142 kb on - strand, within BT1933 at 2428.171 kb on + strand, within BT1933 at 2428.172 kb on - strand, within BT1933 at 2428.172 kb on - strand, within BT1933 at 2428.283 kb on - strand at 2428.283 kb on - strand at 2428.287 kb on - strand at 2428.308 kb on - strand at 2428.375 kb on - strand at 2428.395 kb on + strand at 2428.484 kb on + strand at 2428.497 kb on - strand, within BT1934 at 2428.515 kb on - strand, within BT1934 at 2428.519 kb on - strand, within BT1934 at 2428.607 kb on - strand, within BT1934 at 2428.706 kb on + strand, within BT1934 at 2428.706 kb on + strand, within BT1934 at 2428.749 kb on + strand, within BT1934 at 2428.808 kb on - strand, within BT1934 at 2428.838 kb on - strand, within BT1934 at 2428.897 kb on - strand, within BT1934 at 2428.978 kb on + strand, within BT1934 at 2428.978 kb on + strand, within BT1934 at 2428.979 kb on - strand, within BT1934 at 2429.206 kb on - strand, within BT1934 at 2429.256 kb on - strand, within BT1934 at 2429.293 kb on - strand, within BT1934 at 2429.296 kb on + strand, within BT1934 at 2429.297 kb on - strand, within BT1934 at 2429.355 kb on + strand, within BT1934 at 2429.358 kb on - strand, within BT1934 at 2429.398 kb on + strand, within BT1934 at 2429.399 kb on - strand, within BT1934 at 2429.399 kb on - strand, within BT1934 at 2429.399 kb on - strand, within BT1934 at 2429.403 kb on - strand, within BT1934 at 2429.409 kb on - strand, within BT1934 at 2429.419 kb on - strand, within BT1934 at 2429.497 kb on + strand, within BT1934 at 2429.507 kb on + strand, within BT1934 at 2429.588 kb on + strand, within BT1934 at 2429.619 kb on - strand, within BT1934 at 2429.620 kb on + strand, within BT1934 at 2429.620 kb on + strand, within BT1934 at 2429.620 kb on + strand, within BT1934 at 2429.621 kb on - strand, within BT1934 at 2429.692 kb on + strand, within BT1934 at 2429.692 kb on + strand, within BT1934 at 2429.720 kb on + strand, within BT1934 at 2429.728 kb on + strand, within BT1934 at 2429.742 kb on - strand, within BT1934 at 2429.742 kb on - strand, within BT1934 at 2429.825 kb on - strand, within BT1934 at 2430.008 kb on + strand, within BT1934 at 2430.008 kb on + strand, within BT1934 at 2430.009 kb on - strand, within BT1934 at 2430.011 kb on - strand, within BT1934 at 2430.011 kb on - strand, within BT1934 at 2430.044 kb on + strand, within BT1934 at 2430.044 kb on + strand, within BT1934 at 2430.073 kb on + strand, within BT1934 at 2430.074 kb on - strand, within BT1934 at 2430.077 kb on - strand, within BT1934 at 2430.284 kb on - strand at 2430.291 kb on + strand at 2430.367 kb on - strand at 2430.381 kb on - strand at 2430.381 kb on - strand at 2430.398 kb on + strand at 2430.398 kb on + strand at 2430.398 kb on + strand at 2430.464 kb on - strand at 2430.519 kb on - strand at 2430.605 kb on + strand at 2430.624 kb on - strand at 2430.624 kb on - strand at 2430.670 kb on - strand at 2430.697 kb on - strand, within BT1935 at 2430.701 kb on - strand, within BT1935 at 2430.783 kb on - strand, within BT1935 at 2430.866 kb on + strand at 2430.927 kb on - strand, within BT1935 at 2430.944 kb on - strand, within BT1935 at 2431.036 kb on - strand, within BT1935 at 2431.040 kb on - strand, within BT1935 at 2431.040 kb on - strand, within BT1935 at 2431.217 kb on + strand, within BT1935 at 2431.217 kb on + strand, within BT1935 at 2431.217 kb on + strand, within BT1935 at 2431.227 kb on + strand, within BT1935 at 2431.280 kb on + strand, within BT1935 at 2431.281 kb on - strand, within BT1935 at 2431.293 kb on - strand, within BT1935 at 2431.365 kb on - strand, within BT1935 at 2431.369 kb on + strand, within BT1935 at 2431.370 kb on - strand, within BT1935 at 2431.374 kb on + strand, within BT1935 at 2431.375 kb on - strand, within BT1935 at 2431.389 kb on - strand, within BT1935
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae (C) remove 2,427,286 - BT1932 0.17 +0.7 2,427,286 - BT1932 0.17 -3.7 2,427,305 + BT1932 0.20 -0.4 2,427,305 + BT1932 0.20 -1.7 2,427,350 + BT1932 0.26 -0.9 2,427,350 + BT1932 0.26 -2.4 2,427,355 + BT1932 0.27 -0.5 2,427,355 + BT1932 0.27 -0.1 2,427,356 - BT1932 0.27 -2.8 2,427,425 - BT1932 0.37 -2.7 2,427,770 + BT1932 0.86 -1.2 2,427,824 - +1.3 2,427,968 + -1.1 2,427,978 - +0.6 2,427,987 + -0.7 2,427,988 - -2.5 2,428,043 + -1.9 2,428,044 - -2.4 2,428,044 - -2.1 2,428,071 + +0.9 2,428,072 - -1.5 2,428,073 + -0.0 2,428,074 - -1.3 2,428,082 + +0.7 2,428,142 - BT1933 0.29 -1.2 2,428,142 - BT1933 0.29 -3.3 2,428,171 + BT1933 0.40 -2.7 2,428,172 - BT1933 0.40 -0.7 2,428,172 - BT1933 0.40 -1.9 2,428,283 - -2.1 2,428,283 - +0.3 2,428,287 - -1.5 2,428,308 - -0.2 2,428,375 - -1.5 2,428,395 + +3.2 2,428,484 + -0.3 2,428,497 - BT1934 0.10 +1.5 2,428,515 - BT1934 0.11 -0.1 2,428,519 - BT1934 0.11 +0.3 2,428,607 - BT1934 0.15 -0.6 2,428,706 + BT1934 0.20 -1.4 2,428,706 + BT1934 0.20 -0.3 2,428,749 + BT1934 0.22 -0.3 2,428,808 - BT1934 0.25 -0.3 2,428,838 - BT1934 0.26 -1.9 2,428,897 - BT1934 0.29 -0.7 2,428,978 + BT1934 0.32 -1.3 2,428,978 + BT1934 0.32 -4.5 2,428,979 - BT1934 0.33 -1.3 2,429,206 - BT1934 0.43 -0.7 2,429,256 - BT1934 0.45 -0.0 2,429,293 - BT1934 0.47 -2.3 2,429,296 + BT1934 0.47 -0.2 2,429,297 - BT1934 0.47 -0.3 2,429,355 + BT1934 0.50 -0.6 2,429,358 - BT1934 0.50 -0.2 2,429,398 + BT1934 0.52 -1.1 2,429,399 - BT1934 0.52 -1.3 2,429,399 - BT1934 0.52 -0.7 2,429,399 - BT1934 0.52 +1.3 2,429,403 - BT1934 0.52 +0.2 2,429,409 - BT1934 0.53 -1.6 2,429,419 - BT1934 0.53 -3.4 2,429,497 + BT1934 0.57 +0.3 2,429,507 + BT1934 0.57 -4.6 2,429,588 + BT1934 0.61 -0.4 2,429,619 - BT1934 0.62 +0.4 2,429,620 + BT1934 0.62 -3.0 2,429,620 + BT1934 0.62 +0.3 2,429,620 + BT1934 0.62 +0.3 2,429,621 - BT1934 0.62 -0.1 2,429,692 + BT1934 0.66 +0.2 2,429,692 + BT1934 0.66 -2.1 2,429,720 + BT1934 0.67 -0.1 2,429,728 + BT1934 0.67 +0.0 2,429,742 - BT1934 0.68 +0.1 2,429,742 - BT1934 0.68 -0.3 2,429,825 - BT1934 0.72 -2.1 2,430,008 + BT1934 0.81 -0.3 2,430,008 + BT1934 0.81 +0.3 2,430,009 - BT1934 0.81 -0.3 2,430,011 - BT1934 0.81 -0.1 2,430,011 - BT1934 0.81 -3.8 2,430,044 + BT1934 0.82 +0.7 2,430,044 + BT1934 0.82 -0.7 2,430,073 + BT1934 0.84 -1.7 2,430,074 - BT1934 0.84 +0.1 2,430,077 - BT1934 0.84 -2.7 2,430,284 - -1.3 2,430,291 + -0.7 2,430,367 - +0.5 2,430,381 - -2.2 2,430,381 - -3.1 2,430,398 + +1.0 2,430,398 + -0.2 2,430,398 + -0.7 2,430,464 - -0.0 2,430,519 - -1.1 2,430,605 + -0.7 2,430,624 - -0.5 2,430,624 - -0.5 2,430,670 - -1.9 2,430,697 - BT1935 0.10 -2.3 2,430,701 - BT1935 0.10 +1.3 2,430,783 - BT1935 0.14 +0.3 2,430,866 + -1.9 2,430,927 - BT1935 0.20 -0.3 2,430,944 - BT1935 0.21 -2.4 2,431,036 - BT1935 0.25 -2.5 2,431,040 - BT1935 0.26 -0.7 2,431,040 - BT1935 0.26 +0.5 2,431,217 + BT1935 0.34 -0.5 2,431,217 + BT1935 0.34 -1.9 2,431,217 + BT1935 0.34 -1.9 2,431,227 + BT1935 0.34 -1.1 2,431,280 + BT1935 0.37 -1.2 2,431,281 - BT1935 0.37 +0.3 2,431,293 - BT1935 0.37 +0.7 2,431,365 - BT1935 0.40 -3.5 2,431,369 + BT1935 0.41 -0.3 2,431,370 - BT1935 0.41 +0.8 2,431,374 + BT1935 0.41 -4.1 2,431,375 - BT1935 0.41 -0.9 2,431,389 - BT1935 0.41 -1.2
Or see this region's nucleotide sequence