Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3511 and Rru_A3512 are separated by 109 nucleotides Rru_A3512 and Rru_A3513 are separated by 26 nucleotides Rru_A3513 and Rru_A3514 are separated by 139 nucleotides
Rru_A3511: Rru_A3511 - Aconitate hydratase 1 (NCBI), at 4,039,803 to 4,042,478
_A3511
Rru_A3512: Rru_A3512 - Glutamine amidotransferase class-I (NCBI), at 4,042,588 to 4,043,313
_A3512
Rru_A3513: Rru_A3513 - chemotaxis sensory transducer (NCBI), at 4,043,340 to 4,045,097
_A3513
Rru_A3514: Rru_A3514 - DNA polymerase A (NCBI), at 4,045,237 to 4,048,011
_A3514
Position (kb)
4043
4044
4045
4046 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4042.553 kb on + strand at 4042.554 kb on - strand at 4042.678 kb on + strand, within Rru_A3512 at 4042.679 kb on - strand, within Rru_A3512 at 4042.756 kb on + strand, within Rru_A3512 at 4042.771 kb on + strand, within Rru_A3512 at 4042.772 kb on - strand, within Rru_A3512 at 4042.888 kb on + strand, within Rru_A3512 at 4042.889 kb on - strand, within Rru_A3512 at 4042.889 kb on - strand, within Rru_A3512 at 4043.004 kb on + strand, within Rru_A3512 at 4043.004 kb on + strand, within Rru_A3512 at 4043.326 kb on - strand at 4044.721 kb on + strand, within Rru_A3513 at 4044.784 kb on + strand, within Rru_A3513 at 4044.784 kb on + strand, within Rru_A3513 at 4044.785 kb on - strand, within Rru_A3513 at 4044.785 kb on - strand, within Rru_A3513 at 4044.838 kb on + strand, within Rru_A3513 at 4044.838 kb on + strand, within Rru_A3513 at 4044.839 kb on - strand, within Rru_A3513 at 4044.839 kb on - strand, within Rru_A3513 at 4044.858 kb on - strand, within Rru_A3513 at 4045.008 kb on - strand at 4045.008 kb on - strand at 4045.008 kb on - strand at 4045.199 kb on + strand at 4045.200 kb on - strand at 4045.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 4,042,553 + +0.5 4,042,554 - +0.2 4,042,678 + Rru_A3512 0.12 -0.2 4,042,679 - Rru_A3512 0.13 -0.0 4,042,756 + Rru_A3512 0.23 -0.2 4,042,771 + Rru_A3512 0.25 +0.1 4,042,772 - Rru_A3512 0.25 -0.1 4,042,888 + Rru_A3512 0.41 -1.3 4,042,889 - Rru_A3512 0.41 -0.4 4,042,889 - Rru_A3512 0.41 -2.2 4,043,004 + Rru_A3512 0.57 -0.9 4,043,004 + Rru_A3512 0.57 -3.0 4,043,326 - +0.5 4,044,721 + Rru_A3513 0.79 +1.0 4,044,784 + Rru_A3513 0.82 -0.1 4,044,784 + Rru_A3513 0.82 -0.4 4,044,785 - Rru_A3513 0.82 -1.0 4,044,785 - Rru_A3513 0.82 -0.7 4,044,838 + Rru_A3513 0.85 -1.7 4,044,838 + Rru_A3513 0.85 +0.8 4,044,839 - Rru_A3513 0.85 +0.4 4,044,839 - Rru_A3513 0.85 -0.3 4,044,858 - Rru_A3513 0.86 -2.3 4,045,008 - +0.3 4,045,008 - -0.4 4,045,008 - -1.2 4,045,199 + -0.5 4,045,200 - -2.4 4,045,200 - +1.2
Or see this region's nucleotide sequence