Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0230 and Rru_A0231 are separated by 15 nucleotides Rru_A0231 and Rru_A0232 are separated by 126 nucleotides Rru_A0232 and Rru_A0233 are separated by 144 nucleotides
Rru_A0230: Rru_A0230 - Asparagine synthase, glutamine-hydrolyzing (NCBI), at 282,580 to 284,556
_A0230
Rru_A0231: Rru_A0231 - hypothetical protein (NCBI), at 284,572 to 285,180
_A0231
Rru_A0232: Rru_A0232 - hypothetical protein (NCBI), at 285,307 to 288,021
_A0232
Rru_A0233: Rru_A0233 - hypothetical protein (NCBI), at 288,166 to 289,485
_A0233
Position (kb) 285
286
287
288
289 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 284.573 kb on + strand at 284.574 kb on - strand at 284.675 kb on + strand, within Rru_A0231 at 284.675 kb on + strand, within Rru_A0231 at 284.676 kb on - strand, within Rru_A0231 at 284.676 kb on - strand, within Rru_A0231 at 284.676 kb on - strand, within Rru_A0231 at 284.856 kb on - strand, within Rru_A0231 at 284.903 kb on + strand, within Rru_A0231 at 284.903 kb on + strand, within Rru_A0231 at 284.903 kb on + strand, within Rru_A0231 at 284.903 kb on + strand, within Rru_A0231 at 284.903 kb on + strand, within Rru_A0231 at 284.904 kb on - strand, within Rru_A0231 at 284.904 kb on - strand, within Rru_A0231 at 284.904 kb on - strand, within Rru_A0231 at 284.928 kb on - strand, within Rru_A0231 at 284.964 kb on - strand, within Rru_A0231 at 284.964 kb on - strand, within Rru_A0231 at 284.984 kb on + strand, within Rru_A0231 at 284.985 kb on - strand, within Rru_A0231 at 284.985 kb on - strand, within Rru_A0231 at 284.985 kb on - strand, within Rru_A0231 at 285.161 kb on - strand at 285.311 kb on + strand at 285.312 kb on - strand at 285.312 kb on - strand at 285.312 kb on - strand at 286.262 kb on - strand, within Rru_A0232 at 286.262 kb on - strand, within Rru_A0232 at 286.661 kb on + strand, within Rru_A0232 at 286.661 kb on + strand, within Rru_A0232 at 286.661 kb on + strand, within Rru_A0232 at 286.661 kb on + strand, within Rru_A0232 at 286.662 kb on - strand, within Rru_A0232 at 286.662 kb on - strand, within Rru_A0232 at 286.662 kb on - strand, within Rru_A0232 at 286.662 kb on - strand, within Rru_A0232 at 286.976 kb on + strand, within Rru_A0232 at 286.976 kb on + strand, within Rru_A0232 at 286.976 kb on + strand, within Rru_A0232 at 286.977 kb on - strand, within Rru_A0232 at 286.977 kb on - strand, within Rru_A0232 at 287.097 kb on + strand, within Rru_A0232 at 287.097 kb on + strand, within Rru_A0232 at 287.242 kb on + strand, within Rru_A0232 at 287.360 kb on + strand, within Rru_A0232 at 287.435 kb on - strand, within Rru_A0232 at 287.783 kb on - strand at 287.842 kb on + strand at 287.843 kb on - strand at 287.843 kb on - strand at 288.109 kb on + strand at 288.109 kb on + strand at 288.167 kb on + strand at 288.168 kb on - strand at 288.168 kb on - strand at 288.179 kb on - strand at 288.223 kb on + strand at 288.224 kb on - strand at 288.224 kb on - strand at 288.247 kb on - strand at 288.247 kb on - strand at 288.485 kb on - strand, within Rru_A0233 at 288.485 kb on - strand, within Rru_A0233 at 288.612 kb on + strand, within Rru_A0233 at 288.612 kb on + strand, within Rru_A0233 at 288.613 kb on - strand, within Rru_A0233 at 288.613 kb on - strand, within Rru_A0233 at 288.879 kb on + strand, within Rru_A0233 at 288.880 kb on - strand, within Rru_A0233 at 288.880 kb on - strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.971 kb on - strand, within Rru_A0233
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 284,573 + +0.0 284,574 - -1.5 284,675 + Rru_A0231 0.17 +0.2 284,675 + Rru_A0231 0.17 +0.0 284,676 - Rru_A0231 0.17 -2.2 284,676 - Rru_A0231 0.17 +0.4 284,676 - Rru_A0231 0.17 -3.8 284,856 - Rru_A0231 0.47 +0.1 284,903 + Rru_A0231 0.54 -1.3 284,903 + Rru_A0231 0.54 +0.2 284,903 + Rru_A0231 0.54 -3.5 284,903 + Rru_A0231 0.54 -0.1 284,903 + Rru_A0231 0.54 +1.1 284,904 - Rru_A0231 0.55 +0.9 284,904 - Rru_A0231 0.55 -0.6 284,904 - Rru_A0231 0.55 -2.0 284,928 - Rru_A0231 0.58 +0.5 284,964 - Rru_A0231 0.64 +0.2 284,964 - Rru_A0231 0.64 -1.5 284,984 + Rru_A0231 0.68 -1.4 284,985 - Rru_A0231 0.68 -0.4 284,985 - Rru_A0231 0.68 -0.0 284,985 - Rru_A0231 0.68 -0.1 285,161 - -2.2 285,311 + -2.0 285,312 - -2.3 285,312 - -0.8 285,312 - +0.0 286,262 - Rru_A0232 0.35 +0.5 286,262 - Rru_A0232 0.35 -3.0 286,661 + Rru_A0232 0.50 +0.2 286,661 + Rru_A0232 0.50 -1.2 286,661 + Rru_A0232 0.50 -0.3 286,661 + Rru_A0232 0.50 +0.7 286,662 - Rru_A0232 0.50 -0.5 286,662 - Rru_A0232 0.50 -0.1 286,662 - Rru_A0232 0.50 -2.8 286,662 - Rru_A0232 0.50 +0.2 286,976 + Rru_A0232 0.61 +3.9 286,976 + Rru_A0232 0.61 -1.2 286,976 + Rru_A0232 0.61 -1.7 286,977 - Rru_A0232 0.62 -3.3 286,977 - Rru_A0232 0.62 -1.6 287,097 + Rru_A0232 0.66 -3.3 287,097 + Rru_A0232 0.66 -1.8 287,242 + Rru_A0232 0.71 -0.7 287,360 + Rru_A0232 0.76 -0.3 287,435 - Rru_A0232 0.78 -0.5 287,783 - +0.6 287,842 + +2.3 287,843 - +0.2 287,843 - -0.2 288,109 + -0.2 288,109 + +0.2 288,167 + -1.1 288,168 - +0.3 288,168 - +1.1 288,179 - -0.0 288,223 + -0.5 288,224 - -0.6 288,224 - +0.6 288,247 - +1.1 288,247 - +0.4 288,485 - Rru_A0233 0.24 +0.8 288,485 - Rru_A0233 0.24 +0.3 288,612 + Rru_A0233 0.34 -0.7 288,612 + Rru_A0233 0.34 -0.7 288,613 - Rru_A0233 0.34 -0.8 288,613 - Rru_A0233 0.34 -0.2 288,879 + Rru_A0233 0.54 -1.7 288,880 - Rru_A0233 0.54 +1.0 288,880 - Rru_A0233 0.54 -0.3 288,970 + Rru_A0233 0.61 -0.0 288,970 + Rru_A0233 0.61 -0.7 288,970 + Rru_A0233 0.61 -0.2 288,971 - Rru_A0233 0.61 +1.0
Or see this region's nucleotide sequence