Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2295 and Psyr_2296 are separated by 97 nucleotides Psyr_2296 and Psyr_2297 are separated by 66 nucleotides Psyr_2297 and Psyr_2298 overlap by 8 nucleotides
Psyr_2295: Psyr_2295 - ABC transporter, transmembrane region:ABC transporter, at 2,666,274 to 2,668,085
_2295
Psyr_2296: Psyr_2296 - Aldo/keto reductase, at 2,668,183 to 2,669,220
_2296
Psyr_2297: Psyr_2297 - glucose 1-dehydrogenase, at 2,669,287 to 2,670,087
_2297
Psyr_2298: Psyr_2298 - Glycoside hydrolase, family 15, at 2,670,080 to 2,671,906
_2298
Position (kb)
2668
2669
2670 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2667.238 kb on - strand, within Psyr_2295 at 2667.268 kb on - strand, within Psyr_2295 at 2667.268 kb on - strand, within Psyr_2295 at 2667.268 kb on - strand, within Psyr_2295 at 2667.273 kb on + strand, within Psyr_2295 at 2667.493 kb on - strand, within Psyr_2295 at 2667.810 kb on + strand, within Psyr_2295 at 2667.810 kb on + strand, within Psyr_2295 at 2667.906 kb on + strand at 2668.087 kb on + strand at 2668.088 kb on - strand at 2668.156 kb on - strand at 2668.340 kb on - strand, within Psyr_2296 at 2668.366 kb on + strand, within Psyr_2296 at 2668.367 kb on - strand, within Psyr_2296 at 2668.367 kb on - strand, within Psyr_2296 at 2668.367 kb on - strand, within Psyr_2296 at 2668.408 kb on + strand, within Psyr_2296 at 2668.409 kb on - strand, within Psyr_2296 at 2668.802 kb on - strand, within Psyr_2296 at 2668.876 kb on + strand, within Psyr_2296 at 2668.912 kb on + strand, within Psyr_2296 at 2668.913 kb on - strand, within Psyr_2296 at 2668.973 kb on - strand, within Psyr_2296 at 2669.008 kb on + strand, within Psyr_2296 at 2669.063 kb on - strand, within Psyr_2296 at 2669.063 kb on - strand, within Psyr_2296 at 2669.259 kb on + strand at 2669.259 kb on + strand at 2669.260 kb on - strand at 2669.260 kb on - strand at 2669.262 kb on + strand at 2669.262 kb on + strand at 2669.262 kb on + strand at 2669.262 kb on + strand at 2669.263 kb on - strand at 2669.263 kb on - strand at 2669.398 kb on + strand, within Psyr_2297 at 2669.399 kb on - strand, within Psyr_2297 at 2669.399 kb on - strand, within Psyr_2297 at 2669.617 kb on - strand, within Psyr_2297 at 2669.780 kb on - strand, within Psyr_2297 at 2670.016 kb on + strand at 2670.017 kb on - strand at 2670.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 2,667,238 - Psyr_2295 0.53 +2.2 2,667,268 - Psyr_2295 0.55 +2.9 2,667,268 - Psyr_2295 0.55 -1.2 2,667,268 - Psyr_2295 0.55 -2.0 2,667,273 + Psyr_2295 0.55 +3.5 2,667,493 - Psyr_2295 0.67 +3.8 2,667,810 + Psyr_2295 0.85 +0.9 2,667,810 + Psyr_2295 0.85 +2.2 2,667,906 + -0.9 2,668,087 + +4.2 2,668,088 - +1.9 2,668,156 - +1.9 2,668,340 - Psyr_2296 0.15 +2.2 2,668,366 + Psyr_2296 0.18 -0.1 2,668,367 - Psyr_2296 0.18 +0.9 2,668,367 - Psyr_2296 0.18 -1.2 2,668,367 - Psyr_2296 0.18 -0.1 2,668,408 + Psyr_2296 0.22 +3.8 2,668,409 - Psyr_2296 0.22 -0.1 2,668,802 - Psyr_2296 0.60 +3.9 2,668,876 + Psyr_2296 0.67 +1.5 2,668,912 + Psyr_2296 0.70 -2.0 2,668,913 - Psyr_2296 0.70 -2.1 2,668,973 - Psyr_2296 0.76 +0.1 2,669,008 + Psyr_2296 0.79 +0.5 2,669,063 - Psyr_2296 0.85 +3.7 2,669,063 - Psyr_2296 0.85 +3.0 2,669,259 + -1.5 2,669,259 + -1.6 2,669,260 - -1.9 2,669,260 - +0.0 2,669,262 + +3.5 2,669,262 + -0.1 2,669,262 + -3.9 2,669,262 + -0.8 2,669,263 - +3.5 2,669,263 - +2.2 2,669,398 + Psyr_2297 0.14 +3.6 2,669,399 - Psyr_2297 0.14 -1.1 2,669,399 - Psyr_2297 0.14 -4.1 2,669,617 - Psyr_2297 0.41 +2.5 2,669,780 - Psyr_2297 0.62 -1.0 2,670,016 + -0.7 2,670,017 - -1.5 2,670,017 - +3.8
Or see this region's nucleotide sequence