Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1211 and Psyr_1212 are separated by 6 nucleotides Psyr_1212 and Psyr_1213 are separated by 2 nucleotides Psyr_1213 and Psyr_1214 overlap by 4 nucleotides
Psyr_1211: Psyr_1211 - type III secretion protein HrpP, at 1,368,029 to 1,368,601
_1211
Psyr_1212: Psyr_1212 - type III secretion protein HrpO, at 1,368,608 to 1,369,054
_1212
Psyr_1213: Psyr_1213 - type III secretion cytoplasmic ATPase HrcN, at 1,369,057 to 1,370,406
_1213
Psyr_1214: Psyr_1214 - type III secretion protein HrpQ, at 1,370,403 to 1,371,377
_1214
Position (kb)
1369
1370
1371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1368.362 kb on + strand, within Psyr_1211 at 1368.362 kb on + strand, within Psyr_1211 at 1368.363 kb on - strand, within Psyr_1211 at 1368.363 kb on - strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.609 kb on + strand at 1368.610 kb on - strand at 1368.716 kb on + strand, within Psyr_1212 at 1368.862 kb on + strand, within Psyr_1212 at 1368.863 kb on - strand, within Psyr_1212 at 1368.905 kb on + strand, within Psyr_1212 at 1368.906 kb on - strand, within Psyr_1212 at 1369.151 kb on - strand at 1369.151 kb on - strand at 1369.152 kb on + strand at 1369.206 kb on - strand, within Psyr_1213 at 1369.239 kb on + strand, within Psyr_1213 at 1369.239 kb on + strand, within Psyr_1213 at 1369.240 kb on - strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.242 kb on - strand, within Psyr_1213 at 1369.242 kb on - strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.379 kb on - strand, within Psyr_1213 at 1369.379 kb on - strand, within Psyr_1213 at 1369.450 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.523 kb on + strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.086 kb on + strand, within Psyr_1213 at 1370.164 kb on - strand, within Psyr_1213 at 1370.351 kb on + strand at 1370.352 kb on - strand at 1370.493 kb on + strand at 1370.493 kb on + strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.494 kb on - strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.497 kb on + strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.498 kb on - strand at 1370.620 kb on + strand, within Psyr_1214 at 1370.844 kb on - strand, within Psyr_1214 at 1371.366 kb on + strand at 1371.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 1,368,362 + Psyr_1211 0.58 +2.3 1,368,362 + Psyr_1211 0.58 +2.0 1,368,363 - Psyr_1211 0.58 -1.0 1,368,363 - Psyr_1211 0.58 -2.0 1,368,455 + Psyr_1211 0.74 -0.9 1,368,455 + Psyr_1211 0.74 +4.2 1,368,455 + Psyr_1211 0.74 -1.6 1,368,455 + Psyr_1211 0.74 +2.5 1,368,455 + Psyr_1211 0.74 +0.1 1,368,455 + Psyr_1211 0.74 +1.0 1,368,455 + Psyr_1211 0.74 +2.5 1,368,455 + Psyr_1211 0.74 -0.9 1,368,455 + Psyr_1211 0.74 -2.0 1,368,456 - Psyr_1211 0.75 +2.0 1,368,456 - Psyr_1211 0.75 -2.1 1,368,456 - Psyr_1211 0.75 +0.3 1,368,456 - Psyr_1211 0.75 +1.0 1,368,456 - Psyr_1211 0.75 +1.0 1,368,609 + -0.4 1,368,610 - -0.0 1,368,716 + Psyr_1212 0.24 -3.0 1,368,862 + Psyr_1212 0.57 -1.9 1,368,863 - Psyr_1212 0.57 +3.1 1,368,905 + Psyr_1212 0.66 +3.1 1,368,906 - Psyr_1212 0.67 +2.3 1,369,151 - -1.0 1,369,151 - +3.9 1,369,152 + +0.3 1,369,206 - Psyr_1213 0.11 -1.3 1,369,239 + Psyr_1213 0.13 +0.3 1,369,239 + Psyr_1213 0.13 +2.2 1,369,240 - Psyr_1213 0.14 -0.2 1,369,241 + Psyr_1213 0.14 +3.3 1,369,241 + Psyr_1213 0.14 -0.8 1,369,241 + Psyr_1213 0.14 -1.4 1,369,242 - Psyr_1213 0.14 -0.7 1,369,242 - Psyr_1213 0.14 -1.2 1,369,378 + Psyr_1213 0.24 -0.6 1,369,378 + Psyr_1213 0.24 -1.3 1,369,378 + Psyr_1213 0.24 +0.8 1,369,379 - Psyr_1213 0.24 -0.9 1,369,379 - Psyr_1213 0.24 -0.0 1,369,450 + Psyr_1213 0.29 +2.5 1,369,457 + Psyr_1213 0.30 +2.0 1,369,457 + Psyr_1213 0.30 +0.2 1,369,457 + Psyr_1213 0.30 -2.4 1,369,523 + Psyr_1213 0.35 +4.2 1,369,524 - Psyr_1213 0.35 -1.3 1,369,524 - Psyr_1213 0.35 +0.8 1,369,524 - Psyr_1213 0.35 -2.4 1,370,086 + Psyr_1213 0.76 +4.3 1,370,086 + Psyr_1213 0.76 +3.1 1,370,086 + Psyr_1213 0.76 -1.2 1,370,164 - Psyr_1213 0.82 +0.4 1,370,351 + +2.5 1,370,352 - -1.5 1,370,493 + +0.9 1,370,493 + +0.6 1,370,494 - +3.3 1,370,494 - +2.5 1,370,494 - -1.4 1,370,494 - +3.4 1,370,494 - +0.6 1,370,497 + -1.7 1,370,497 + -0.3 1,370,497 + +3.7 1,370,497 + -0.6 1,370,497 + +0.7 1,370,498 - -0.2 1,370,498 - +2.0 1,370,498 - -2.2 1,370,498 - +4.4 1,370,498 - +3.3 1,370,498 - +0.1 1,370,498 - -0.6 1,370,498 - -1.1 1,370,620 + Psyr_1214 0.22 -0.2 1,370,844 - Psyr_1214 0.45 -0.0 1,371,366 + +2.5 1,371,366 + -3.0
Or see this region's nucleotide sequence