Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0311 and Psyr_0312 are separated by 119 nucleotides Psyr_0312 and Psyr_0313 are separated by 255 nucleotides Psyr_0313 and Psyr_0314 are separated by 130 nucleotides Psyr_0314 and Psyr_0315 are separated by 6 nucleotides
Psyr_0311: Psyr_0311 - CDS, at 331,803 to 332,150
_0311
Psyr_0312: Psyr_0312 - hypothetical protein, at 332,270 to 332,632
_0312
Psyr_0313: Psyr_0313 - Zinc-containing alcohol dehydrogenase superfamily, at 332,888 to 333,874
_0313
Psyr_0314: Psyr_0314 - Flagellar basal body-associated protein FliL, at 334,005 to 334,412
_0314
Psyr_0315: Psyr_0315 - Protein of unknown function DUF589, at 334,419 to 334,868
_0315
Position (kb) 332
333
334 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 332.230 kb on + strand at 332.230 kb on + strand at 332.233 kb on + strand at 332.264 kb on + strand at 332.338 kb on - strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.359 kb on + strand, within Psyr_0312 at 332.360 kb on - strand, within Psyr_0312 at 332.362 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.488 kb on + strand, within Psyr_0312 at 332.495 kb on + strand, within Psyr_0312 at 332.495 kb on + strand, within Psyr_0312 at 332.550 kb on + strand, within Psyr_0312 at 332.550 kb on + strand, within Psyr_0312 at 332.551 kb on - strand, within Psyr_0312 at 332.551 kb on - strand, within Psyr_0312 at 332.565 kb on - strand, within Psyr_0312 at 332.565 kb on - strand, within Psyr_0312 at 332.637 kb on + strand at 332.645 kb on - strand at 332.647 kb on + strand at 332.647 kb on + strand at 332.647 kb on + strand at 332.648 kb on - strand at 332.648 kb on - strand at 332.648 kb on - strand at 332.709 kb on + strand at 332.709 kb on + strand at 332.712 kb on + strand at 332.712 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.717 kb on - strand at 332.749 kb on + strand at 332.749 kb on + strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.773 kb on - strand at 332.832 kb on + strand at 332.878 kb on + strand at 332.879 kb on - strand at 332.882 kb on + strand at 333.215 kb on + strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.237 kb on + strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.440 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.861 kb on - strand at 333.861 kb on - strand at 333.914 kb on + strand at 333.914 kb on + strand at 333.915 kb on - strand at 333.915 kb on - strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.970 kb on + strand at 333.970 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.989 kb on + strand at 333.989 kb on + strand at 333.990 kb on - strand at 333.992 kb on + strand at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.394 kb on + strand at 334.395 kb on - strand at 334.531 kb on + strand, within Psyr_0315 at 334.661 kb on - strand, within Psyr_0315 at 334.720 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.775 kb on - strand, within Psyr_0315
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 332,230 + -2.1 332,230 + -1.8 332,233 + +1.1 332,264 + -1.5 332,338 - Psyr_0312 0.19 +2.9 332,356 + Psyr_0312 0.24 -2.2 332,356 + Psyr_0312 0.24 -0.5 332,356 + Psyr_0312 0.24 +0.1 332,359 + Psyr_0312 0.25 -3.6 332,360 - Psyr_0312 0.25 -3.0 332,362 - Psyr_0312 0.25 -0.4 332,452 - Psyr_0312 0.50 +3.7 332,452 - Psyr_0312 0.50 -0.9 332,452 - Psyr_0312 0.50 +1.1 332,488 + Psyr_0312 0.60 -1.7 332,495 + Psyr_0312 0.62 -1.6 332,495 + Psyr_0312 0.62 +1.1 332,550 + Psyr_0312 0.77 -2.5 332,550 + Psyr_0312 0.77 -2.2 332,551 - Psyr_0312 0.77 +2.9 332,551 - Psyr_0312 0.77 +2.9 332,565 - Psyr_0312 0.81 -1.8 332,565 - Psyr_0312 0.81 -2.1 332,637 + -3.3 332,645 - -1.3 332,647 + +1.7 332,647 + +2.9 332,647 + +3.6 332,648 - +1.1 332,648 - -1.2 332,648 - -0.3 332,709 + +2.1 332,709 + -2.7 332,712 + -4.0 332,712 + +2.9 332,716 + -2.6 332,716 + -1.1 332,716 + +3.1 332,716 + +1.9 332,717 - -1.5 332,749 + +0.7 332,749 + -2.1 332,772 + +1.7 332,772 + +0.1 332,773 - -1.3 332,832 + -1.3 332,878 + +3.4 332,879 - -0.7 332,882 + -1.5 333,215 + Psyr_0313 0.33 -3.0 333,216 - Psyr_0313 0.33 +4.4 333,216 - Psyr_0313 0.33 +0.1 333,216 - Psyr_0313 0.33 +3.8 333,237 + Psyr_0313 0.35 +1.7 333,238 - Psyr_0313 0.35 -0.5 333,238 - Psyr_0313 0.35 -1.3 333,238 - Psyr_0313 0.35 -1.1 333,238 - Psyr_0313 0.35 -1.6 333,303 + Psyr_0313 0.42 +0.1 333,303 + Psyr_0313 0.42 +1.1 333,303 + Psyr_0313 0.42 -1.0 333,303 + Psyr_0313 0.42 -1.7 333,303 + Psyr_0313 0.42 +2.7 333,304 - Psyr_0313 0.42 +2.4 333,304 - Psyr_0313 0.42 -0.7 333,304 - Psyr_0313 0.42 +3.1 333,304 - Psyr_0313 0.42 -0.8 333,440 + Psyr_0313 0.56 +1.0 333,567 + Psyr_0313 0.69 +3.3 333,567 + Psyr_0313 0.69 -1.7 333,568 - Psyr_0313 0.69 -0.2 333,568 - Psyr_0313 0.69 -1.7 333,568 - Psyr_0313 0.69 +2.4 333,723 + Psyr_0313 0.85 +0.1 333,723 + Psyr_0313 0.85 -0.0 333,724 - Psyr_0313 0.85 -0.9 333,724 - Psyr_0313 0.85 +4.0 333,724 - Psyr_0313 0.85 +0.3 333,724 - Psyr_0313 0.85 +3.6 333,861 - -0.5 333,861 - -0.4 333,914 + +1.1 333,914 + -0.3 333,915 - -1.1 333,915 - -2.5 333,963 + -1.3 333,963 + +1.7 333,963 + +1.8 333,963 + +2.1 333,963 + -0.5 333,963 + -2.7 333,963 + -0.8 333,963 + +1.4 333,963 + +3.9 333,963 + -0.4 333,963 + +2.4 333,963 + +2.9 333,963 + +2.7 333,963 + +2.7 333,964 - +2.4 333,964 - +0.1 333,964 - -0.2 333,964 - +3.6 333,964 - +0.4 333,964 - -1.1 333,964 - -0.9 333,964 - -1.4 333,964 - +4.2 333,964 - +0.1 333,964 - -1.8 333,964 - -2.0 333,964 - -1.8 333,964 - -0.6 333,964 - -2.4 333,964 - +0.2 333,964 - -4.1 333,964 - -1.1 333,964 - -0.3 333,964 - -2.1 333,964 - -2.4 333,964 - +0.1 333,964 - -1.0 333,964 - -0.1 333,964 - +1.7 333,970 + +2.7 333,970 + -0.5 333,975 + +1.6 333,975 + +0.2 333,975 + +2.4 333,975 + +0.4 333,975 + +2.9 333,975 + -1.5 333,975 + -1.0 333,975 + +0.1 333,975 + -1.4 333,975 + -1.0 333,975 + -2.4 333,975 + +1.0 333,976 - -0.4 333,976 - -0.1 333,976 - +2.4 333,976 - -0.0 333,976 - -0.9 333,989 + -1.5 333,989 + +0.1 333,990 - +1.7 333,992 + -0.1 334,098 + Psyr_0314 0.23 -1.1 334,098 + Psyr_0314 0.23 +0.1 334,098 + Psyr_0314 0.23 -3.6 334,098 + Psyr_0314 0.23 -0.5 334,098 + Psyr_0314 0.23 +0.0 334,099 - Psyr_0314 0.23 -1.6 334,099 - Psyr_0314 0.23 -4.5 334,099 - Psyr_0314 0.23 -1.2 334,099 - Psyr_0314 0.23 +3.4 334,131 + Psyr_0314 0.31 -1.5 334,131 + Psyr_0314 0.31 -4.0 334,131 + Psyr_0314 0.31 +2.9 334,131 + Psyr_0314 0.31 +3.1 334,132 - Psyr_0314 0.31 -1.3 334,132 - Psyr_0314 0.31 +2.7 334,132 - Psyr_0314 0.31 -2.2 334,132 - Psyr_0314 0.31 +1.5 334,132 - Psyr_0314 0.31 +1.7 334,164 + Psyr_0314 0.39 +2.4 334,164 + Psyr_0314 0.39 +1.1 334,164 + Psyr_0314 0.39 -1.1 334,164 + Psyr_0314 0.39 -2.3 334,164 + Psyr_0314 0.39 +2.4 334,165 - Psyr_0314 0.39 -0.9 334,165 - Psyr_0314 0.39 -2.5 334,165 - Psyr_0314 0.39 -0.3 334,165 - Psyr_0314 0.39 +3.1 334,394 + +1.1 334,395 - +2.7 334,531 + Psyr_0315 0.25 -1.5 334,661 - Psyr_0315 0.54 -0.8 334,720 + Psyr_0315 0.67 +0.1 334,774 + Psyr_0315 0.79 -3.3 334,774 + Psyr_0315 0.79 +2.9 334,774 + Psyr_0315 0.79 +1.0 334,774 + Psyr_0315 0.79 +4.0 334,775 - Psyr_0315 0.79 +2.1
Or see this region's nucleotide sequence