Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0224 and Psyr_0225 are separated by 11 nucleotides Psyr_0225 and Psyr_0226 are separated by 81 nucleotides Psyr_0226 and Psyr_0227 are separated by 233 nucleotides Psyr_0227 and Psyr_0228 are separated by 242 nucleotides
Psyr_0224: Psyr_0224 - LSU ribosomal protein L28P, at 239,211 to 239,444
_0224
Psyr_0225: Psyr_0225 - LSU ribosomal protein L33P, at 239,456 to 239,611
_0225
Psyr_0226: Psyr_0226 - Protein of unknown function DUF861, at 239,693 to 240,061
_0226
Psyr_0227: Psyr_0227 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase, at 240,295 to 241,794
_0227
Psyr_0228: Psyr_0228 - Major facilitator superfamily, at 242,037 to 243,185
_0228
Position (kb) 240
241
242 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 239.690 kb on + strand at 239.691 kb on - strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.698 kb on + strand at 239.698 kb on + strand at 239.699 kb on - strand at 239.699 kb on - strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.758 kb on + strand, within Psyr_0226 at 239.758 kb on + strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.877 kb on + strand, within Psyr_0226 at 239.877 kb on + strand, within Psyr_0226 at 239.878 kb on - strand, within Psyr_0226 at 239.882 kb on + strand, within Psyr_0226 at 239.882 kb on + strand, within Psyr_0226 at 239.883 kb on - strand, within Psyr_0226 at 239.946 kb on + strand, within Psyr_0226 at 239.946 kb on + strand, within Psyr_0226 at 239.947 kb on - strand, within Psyr_0226 at 239.947 kb on - strand, within Psyr_0226 at 239.955 kb on + strand, within Psyr_0226 at 239.956 kb on - strand, within Psyr_0226 at 239.956 kb on - strand, within Psyr_0226 at 240.007 kb on - strand, within Psyr_0226 at 240.146 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.158 kb on - strand at 240.158 kb on - strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.180 kb on - strand at 240.180 kb on - strand at 240.180 kb on - strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.190 kb on + strand at 240.215 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.383 kb on - strand at 240.383 kb on - strand at 240.663 kb on - strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.716 kb on - strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 241.002 kb on + strand, within Psyr_0227 at 241.003 kb on - strand, within Psyr_0227 at 241.063 kb on + strand, within Psyr_0227 at 241.063 kb on + strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.353 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.368 kb on + strand, within Psyr_0227 at 241.368 kb on + strand, within Psyr_0227 at 241.383 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.599 kb on - strand, within Psyr_0227 at 241.659 kb on - strand at 241.659 kb on - strand at 241.672 kb on + strand at 241.759 kb on + strand at 241.759 kb on + strand at 241.759 kb on + strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.792 kb on + strand at 241.792 kb on + strand at 241.792 kb on + strand at 241.793 kb on - strand at 241.793 kb on - strand at 241.793 kb on - strand at 241.991 kb on + strand at 241.991 kb on + strand at 241.992 kb on - strand at 241.992 kb on - strand at 242.049 kb on - strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.307 kb on + strand, within Psyr_0228 at 242.310 kb on + strand, within Psyr_0228 at 242.433 kb on + strand, within Psyr_0228 at 242.445 kb on + strand, within Psyr_0228 at 242.445 kb on + strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.703 kb on + strand, within Psyr_0228 at 242.704 kb on - strand, within Psyr_0228 at 242.711 kb on - strand, within Psyr_0228 at 242.711 kb on - strand, within Psyr_0228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 239,690 + +1.0 239,691 - +0.2 239,694 + -0.8 239,694 + -0.1 239,694 + -0.5 239,694 + +3.3 239,694 + -1.5 239,694 + -1.2 239,695 - -1.4 239,695 - -2.3 239,695 - +1.0 239,695 - +0.2 239,695 - -0.8 239,695 - -0.5 239,698 + -0.5 239,698 + +0.6 239,699 - -0.7 239,699 - +3.7 239,724 + +0.9 239,724 + -0.1 239,724 + -2.4 239,725 - -2.3 239,725 - -0.4 239,725 - -0.4 239,725 - -0.1 239,725 - +2.2 239,725 - +1.7 239,758 + Psyr_0226 0.18 +1.2 239,758 + Psyr_0226 0.18 -0.3 239,759 - Psyr_0226 0.18 +0.2 239,759 - Psyr_0226 0.18 +0.1 239,759 - Psyr_0226 0.18 -0.2 239,862 + Psyr_0226 0.46 +2.5 239,862 + Psyr_0226 0.46 +2.9 239,862 + Psyr_0226 0.46 +4.3 239,863 - Psyr_0226 0.46 -2.7 239,863 - Psyr_0226 0.46 -2.9 239,863 - Psyr_0226 0.46 -0.4 239,863 - Psyr_0226 0.46 +1.3 239,877 + Psyr_0226 0.50 +7.9 239,877 + Psyr_0226 0.50 -1.7 239,878 - Psyr_0226 0.50 +0.2 239,882 + Psyr_0226 0.51 +6.2 239,882 + Psyr_0226 0.51 +2.8 239,883 - Psyr_0226 0.51 +3.0 239,946 + Psyr_0226 0.69 +5.7 239,946 + Psyr_0226 0.69 +10.7 239,947 - Psyr_0226 0.69 -0.1 239,947 - Psyr_0226 0.69 -1.8 239,955 + Psyr_0226 0.71 +5.7 239,956 - Psyr_0226 0.71 +0.2 239,956 - Psyr_0226 0.71 -1.3 240,007 - Psyr_0226 0.85 -1.7 240,146 + +5.2 240,157 + +7.0 240,157 + +6.7 240,157 + +11.7 240,157 + +5.5 240,157 + +6.3 240,157 + +7.7 240,158 - -4.5 240,158 - -0.7 240,179 + +5.1 240,179 + +6.3 240,179 + +5.9 240,179 + +9.6 240,180 - +1.2 240,180 - -2.4 240,180 - +1.7 240,188 + +4.8 240,188 + +8.0 240,188 + +9.4 240,188 + +9.2 240,188 + +4.0 240,189 - -2.6 240,189 - +2.2 240,189 - -0.8 240,189 - -3.6 240,190 + +8.6 240,215 + +0.1 240,382 + +0.2 240,382 + -1.3 240,382 + -3.2 240,382 + +3.3 240,382 + -1.5 240,383 - +1.2 240,383 - -0.8 240,663 - Psyr_0227 0.25 -1.7 240,715 + Psyr_0227 0.28 +2.7 240,715 + Psyr_0227 0.28 -0.1 240,715 + Psyr_0227 0.28 -1.2 240,715 + Psyr_0227 0.28 -2.0 240,715 + Psyr_0227 0.28 -2.9 240,715 + Psyr_0227 0.28 +1.7 240,715 + Psyr_0227 0.28 -2.4 240,716 - Psyr_0227 0.28 -0.6 240,956 + Psyr_0227 0.44 +0.3 240,956 + Psyr_0227 0.44 -2.0 240,956 + Psyr_0227 0.44 -0.2 240,956 + Psyr_0227 0.44 +3.9 240,956 + Psyr_0227 0.44 +3.7 240,957 - Psyr_0227 0.44 -3.8 240,957 - Psyr_0227 0.44 -3.9 240,957 - Psyr_0227 0.44 -4.5 240,957 - Psyr_0227 0.44 -2.3 241,002 + Psyr_0227 0.47 +1.2 241,003 - Psyr_0227 0.47 -2.8 241,063 + Psyr_0227 0.51 -1.1 241,063 + Psyr_0227 0.51 -1.1 241,064 - Psyr_0227 0.51 +2.2 241,064 - Psyr_0227 0.51 +0.3 241,064 - Psyr_0227 0.51 -4.2 241,064 - Psyr_0227 0.51 -1.6 241,353 + Psyr_0227 0.71 +5.3 241,366 + Psyr_0227 0.71 +0.9 241,366 + Psyr_0227 0.71 +0.3 241,366 + Psyr_0227 0.71 +1.2 241,366 + Psyr_0227 0.71 +0.6 241,366 + Psyr_0227 0.71 +0.8 241,366 + Psyr_0227 0.71 -0.6 241,366 + Psyr_0227 0.71 +3.6 241,366 + Psyr_0227 0.71 +1.0 241,366 + Psyr_0227 0.71 +5.4 241,366 + Psyr_0227 0.71 +4.5 241,366 + Psyr_0227 0.71 +2.5 241,367 - Psyr_0227 0.71 +1.2 241,367 - Psyr_0227 0.71 -1.4 241,367 - Psyr_0227 0.71 +0.2 241,367 - Psyr_0227 0.71 +0.2 241,367 - Psyr_0227 0.71 -2.8 241,367 - Psyr_0227 0.71 +0.2 241,367 - Psyr_0227 0.71 -2.5 241,367 - Psyr_0227 0.71 -2.3 241,368 + Psyr_0227 0.72 +0.8 241,368 + Psyr_0227 0.72 +1.4 241,383 + Psyr_0227 0.73 +1.9 241,444 + Psyr_0227 0.77 +0.3 241,444 + Psyr_0227 0.77 -0.1 241,444 + Psyr_0227 0.77 +3.7 241,444 + Psyr_0227 0.77 +3.5 241,444 + Psyr_0227 0.77 -0.4 241,444 + Psyr_0227 0.77 +1.4 241,444 + Psyr_0227 0.77 -0.5 241,444 + Psyr_0227 0.77 +3.5 241,444 + Psyr_0227 0.77 +1.2 241,445 - Psyr_0227 0.77 -2.6 241,445 - Psyr_0227 0.77 +1.2 241,445 - Psyr_0227 0.77 -4.7 241,445 - Psyr_0227 0.77 -5.0 241,445 - Psyr_0227 0.77 -3.2 241,445 - Psyr_0227 0.77 +0.2 241,445 - Psyr_0227 0.77 +2.2 241,599 - Psyr_0227 0.87 -4.2 241,659 - -0.8 241,659 - +2.7 241,672 + +0.6 241,759 + +4.1 241,759 + +5.1 241,759 + +3.8 241,760 - -2.0 241,760 - -1.8 241,760 - -4.2 241,760 - -2.0 241,760 - -3.6 241,792 + +2.1 241,792 + +7.3 241,792 + +1.4 241,793 - +2.7 241,793 - +0.2 241,793 - -1.3 241,991 + +2.5 241,991 + -0.7 241,992 - -1.3 241,992 - -3.9 242,049 - -5.0 242,052 + -3.3 242,052 + -3.3 242,052 + +2.2 242,052 + -2.7 242,052 + -2.2 242,052 + -1.8 242,053 - +0.2 242,053 - -2.0 242,053 - +1.7 242,053 - -2.8 242,053 - +1.2 242,307 + Psyr_0228 0.23 -1.4 242,310 + Psyr_0228 0.24 -1.3 242,433 + Psyr_0228 0.34 +1.7 242,445 + Psyr_0228 0.36 +0.2 242,445 + Psyr_0228 0.36 +2.5 242,446 - Psyr_0228 0.36 -1.6 242,446 - Psyr_0228 0.36 -4.9 242,446 - Psyr_0228 0.36 -2.0 242,446 - Psyr_0228 0.36 -1.2 242,703 + Psyr_0228 0.58 -4.7 242,704 - Psyr_0228 0.58 -3.7 242,711 - Psyr_0228 0.59 +2.2 242,711 - Psyr_0228 0.59 -3.7
Or see this region's nucleotide sequence