Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0081 and Psyr_0082 are separated by 2 nucleotides Psyr_0082 and Psyr_0083 are separated by 13 nucleotides Psyr_0083 and Psyr_0084 are separated by 94 nucleotides
Psyr_0081: Psyr_0081 - ABC transporter, at 87,481 to 88,461
_0081
Psyr_0082: Psyr_0082 - Binding-protein-dependent transport systems inner membrane component, at 88,464 to 89,336
_0082
Psyr_0083: Psyr_0083 - Binding-protein-dependent transport systems inner membrane component, at 89,350 to 90,171
_0083
Psyr_0084: Psyr_0084 - extracellular solute-binding protein, family 1, at 90,266 to 91,276
_0084
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 87.483 kb on - strand at 87.483 kb on - strand at 87.788 kb on + strand, within Psyr_0081 at 87.789 kb on - strand, within Psyr_0081 at 88.465 kb on + strand at 88.465 kb on + strand at 88.466 kb on - strand at 88.482 kb on - strand at 88.588 kb on + strand, within Psyr_0082 at 88.588 kb on + strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.695 kb on - strand, within Psyr_0082 at 88.712 kb on - strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.239 kb on - strand, within Psyr_0082 at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.450 kb on + strand, within Psyr_0083 at 89.450 kb on + strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083 at 89.819 kb on + strand, within Psyr_0083 at 89.819 kb on + strand, within Psyr_0083 at 89.820 kb on - strand, within Psyr_0083 at 89.913 kb on - strand, within Psyr_0083 at 89.970 kb on - strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.064 kb on + strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.065 kb on - strand, within Psyr_0083 at 90.085 kb on + strand, within Psyr_0083 at 90.085 kb on + strand, within Psyr_0083 at 90.088 kb on + strand, within Psyr_0083 at 90.088 kb on + strand, within Psyr_0083 at 90.089 kb on - strand at 90.107 kb on + strand at 90.107 kb on + strand at 90.107 kb on + strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.108 kb on - strand at 90.119 kb on + strand at 90.119 kb on + strand at 90.159 kb on + strand at 90.160 kb on - strand at 90.171 kb on + strand at 90.171 kb on + strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.172 kb on - strand at 90.267 kb on + strand at 90.279 kb on + strand at 90.279 kb on + strand at 90.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 87,483 - +2.5 87,483 - -1.0 87,788 + Psyr_0081 0.31 -0.5 87,789 - Psyr_0081 0.31 -1.2 88,465 + +4.2 88,465 + -0.8 88,466 - -1.1 88,482 - -0.5 88,588 + Psyr_0082 0.14 -1.4 88,588 + Psyr_0082 0.14 +0.3 88,589 - Psyr_0082 0.14 +1.7 88,589 - Psyr_0082 0.14 -1.2 88,589 - Psyr_0082 0.14 +4.1 88,589 - Psyr_0082 0.14 +2.7 88,695 - Psyr_0082 0.26 -0.4 88,712 - Psyr_0082 0.28 -0.9 88,963 + Psyr_0082 0.57 -0.6 88,963 + Psyr_0082 0.57 -1.5 88,963 + Psyr_0082 0.57 +3.2 88,964 - Psyr_0082 0.57 -1.9 88,964 - Psyr_0082 0.57 -1.4 88,964 - Psyr_0082 0.57 -0.7 88,964 - Psyr_0082 0.57 -1.3 89,044 + Psyr_0082 0.66 +3.2 89,044 + Psyr_0082 0.66 +1.5 89,045 - Psyr_0082 0.67 -0.6 89,045 - Psyr_0082 0.67 -2.3 89,239 - Psyr_0082 0.89 -0.0 89,329 + -1.6 89,329 + -0.5 89,329 + +3.5 89,329 + +0.5 89,330 - +2.2 89,330 - +2.4 89,330 - +4.1 89,450 + Psyr_0083 0.12 -2.4 89,450 + Psyr_0083 0.12 +4.0 89,451 - Psyr_0083 0.12 -2.4 89,451 - Psyr_0083 0.12 -2.6 89,819 + Psyr_0083 0.57 -1.3 89,819 + Psyr_0083 0.57 +1.0 89,820 - Psyr_0083 0.57 +2.2 89,913 - Psyr_0083 0.68 +0.9 89,970 - Psyr_0083 0.75 -2.0 90,064 + Psyr_0083 0.87 -1.0 90,064 + Psyr_0083 0.87 -1.3 90,064 + Psyr_0083 0.87 +1.7 90,064 + Psyr_0083 0.87 -0.9 90,064 + Psyr_0083 0.87 +3.6 90,064 + Psyr_0083 0.87 +3.0 90,064 + Psyr_0083 0.87 +4.3 90,064 + Psyr_0083 0.87 -0.5 90,064 + Psyr_0083 0.87 +1.0 90,064 + Psyr_0083 0.87 +0.2 90,065 - Psyr_0083 0.87 -1.0 90,065 - Psyr_0083 0.87 -0.0 90,065 - Psyr_0083 0.87 +0.2 90,065 - Psyr_0083 0.87 -0.5 90,065 - Psyr_0083 0.87 -2.4 90,065 - Psyr_0083 0.87 +3.1 90,065 - Psyr_0083 0.87 +0.3 90,085 + Psyr_0083 0.89 +2.2 90,085 + Psyr_0083 0.89 +0.1 90,088 + Psyr_0083 0.90 -0.3 90,088 + Psyr_0083 0.90 +2.7 90,089 - -0.6 90,107 + -1.6 90,107 + +0.9 90,107 + -0.4 90,108 - -1.8 90,108 - +3.5 90,108 - +0.7 90,108 - -2.2 90,119 + -0.3 90,119 + +0.5 90,159 + +0.8 90,160 - +1.2 90,171 + -0.7 90,171 + +1.2 90,172 - +3.5 90,172 - +3.5 90,172 - +3.8 90,172 - -0.0 90,267 + -1.7 90,279 + -2.8 90,279 + -1.5 90,279 + -0.5
Or see this region's nucleotide sequence