Experiment: KB with Benzalkonium Chloride 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0062 and Psyr_0063 are separated by 232 nucleotides Psyr_0063 and Psyr_0064 overlap by 4 nucleotides Psyr_0064 and Psyr_0065 are separated by 134 nucleotides
Psyr_0062: Psyr_0062 - hydroxymethylbilane synthase, at 61,471 to 62,412
_0062
Psyr_0063: Psyr_0063 - Two-component response regulator AlgR, at 62,645 to 63,391
_0063
Psyr_0064: Psyr_0064 - Sensory transduction protein kinase AlgZ, at 63,388 to 64,470
_0064
Psyr_0065: Psyr_0065 - argininosuccinate lyase, at 64,605 to 65,999
_0065
Position (kb) 63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 62.448 kb on - strand at 62.448 kb on - strand at 62.448 kb on - strand at 62.448 kb on - strand at 62.505 kb on + strand at 62.506 kb on - strand at 62.506 kb on - strand at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.968 kb on + strand, within Psyr_0063 at 62.969 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.433 kb on + strand at 63.434 kb on - strand at 63.443 kb on + strand at 63.443 kb on + strand at 63.443 kb on + strand at 63.443 kb on + strand at 63.514 kb on + strand, within Psyr_0064 at 63.514 kb on + strand, within Psyr_0064 at 63.514 kb on + strand, within Psyr_0064 at 63.515 kb on - strand, within Psyr_0064 at 63.515 kb on - strand, within Psyr_0064 at 63.515 kb on - strand, within Psyr_0064 at 63.515 kb on - strand, within Psyr_0064 at 63.515 kb on - strand, within Psyr_0064 at 63.516 kb on + strand, within Psyr_0064 at 63.516 kb on + strand, within Psyr_0064 at 63.517 kb on - strand, within Psyr_0064 at 63.517 kb on - strand, within Psyr_0064 at 63.517 kb on - strand, within Psyr_0064 at 63.538 kb on - strand, within Psyr_0064 at 63.592 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.611 kb on + strand, within Psyr_0064 at 63.612 kb on - strand, within Psyr_0064 at 63.612 kb on - strand, within Psyr_0064 at 63.612 kb on - strand, within Psyr_0064 at 63.612 kb on - strand, within Psyr_0064 at 63.628 kb on + strand, within Psyr_0064 at 63.629 kb on - strand, within Psyr_0064 at 63.662 kb on + strand, within Psyr_0064 at 63.662 kb on + strand, within Psyr_0064 at 63.662 kb on + strand, within Psyr_0064 at 63.662 kb on + strand, within Psyr_0064 at 63.663 kb on - strand, within Psyr_0064 at 63.682 kb on + strand, within Psyr_0064 at 63.697 kb on + strand, within Psyr_0064 at 63.697 kb on + strand, within Psyr_0064 at 63.752 kb on - strand, within Psyr_0064 at 63.752 kb on - strand, within Psyr_0064 at 63.770 kb on + strand, within Psyr_0064 at 63.784 kb on + strand, within Psyr_0064 at 63.789 kb on - strand, within Psyr_0064 at 64.032 kb on - strand, within Psyr_0064 at 64.037 kb on + strand, within Psyr_0064 at 64.037 kb on + strand, within Psyr_0064 at 64.037 kb on + strand, within Psyr_0064 at 64.038 kb on - strand, within Psyr_0064 at 64.089 kb on - strand at 64.261 kb on + strand, within Psyr_0064 at 64.262 kb on - strand, within Psyr_0064 at 64.262 kb on - strand, within Psyr_0064 at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.522 kb on + strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.523 kb on - strand at 64.538 kb on + strand at 64.539 kb on - strand at 64.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Benzalkonium Chloride 6.25 ug/ml remove 62,448 - +3.7 62,448 - -1.5 62,448 - +0.9 62,448 - -2.9 62,505 + -1.8 62,506 - +4.2 62,506 - +0.4 62,892 + Psyr_0063 0.33 -0.3 62,892 + Psyr_0063 0.33 +3.5 62,892 + Psyr_0063 0.33 -0.5 62,892 + Psyr_0063 0.33 +3.2 62,892 + Psyr_0063 0.33 -1.0 62,892 + Psyr_0063 0.33 -1.0 62,892 + Psyr_0063 0.33 +2.6 62,892 + Psyr_0063 0.33 -2.2 62,893 - Psyr_0063 0.33 +0.2 62,893 - Psyr_0063 0.33 +1.0 62,893 - Psyr_0063 0.33 +0.5 62,893 - Psyr_0063 0.33 -0.9 62,893 - Psyr_0063 0.33 -2.0 62,893 - Psyr_0063 0.33 +0.5 62,893 - Psyr_0063 0.33 +3.3 62,913 + Psyr_0063 0.36 -0.7 62,913 + Psyr_0063 0.36 +3.7 62,913 + Psyr_0063 0.36 -1.3 62,913 + Psyr_0063 0.36 +3.9 62,914 - Psyr_0063 0.36 +4.0 62,914 - Psyr_0063 0.36 +3.5 62,914 - Psyr_0063 0.36 +1.7 62,914 - Psyr_0063 0.36 -0.5 62,914 - Psyr_0063 0.36 +4.5 62,914 - Psyr_0063 0.36 -0.8 62,968 + Psyr_0063 0.43 +2.5 62,969 - Psyr_0063 0.43 +0.1 63,097 - Psyr_0063 0.61 -2.3 63,097 - Psyr_0063 0.61 +0.3 63,097 - Psyr_0063 0.61 +2.2 63,242 + Psyr_0063 0.80 -2.6 63,242 + Psyr_0063 0.80 +1.6 63,242 + Psyr_0063 0.80 +0.2 63,242 + Psyr_0063 0.80 +2.2 63,242 + Psyr_0063 0.80 +3.0 63,242 + Psyr_0063 0.80 -0.6 63,243 - Psyr_0063 0.80 +0.5 63,243 - Psyr_0063 0.80 -2.5 63,243 - Psyr_0063 0.80 -0.8 63,243 - Psyr_0063 0.80 -1.0 63,391 + +3.7 63,391 + +0.4 63,391 + +4.2 63,391 + +3.9 63,391 + -2.1 63,391 + +3.2 63,392 - +0.5 63,392 - -0.5 63,392 - -0.2 63,392 - +1.7 63,393 + -0.8 63,393 + -2.2 63,393 + +3.3 63,393 + +0.2 63,393 + +0.2 63,393 + +1.3 63,393 + +0.3 63,393 + +0.5 63,393 + +1.2 63,393 + +0.4 63,393 + +1.2 63,393 + -0.3 63,393 + +2.2 63,393 + +2.2 63,394 - -0.2 63,394 - -1.6 63,394 - -1.4 63,394 - -2.4 63,394 - -2.3 63,394 - +3.9 63,394 - +0.5 63,394 - -1.0 63,394 - +0.2 63,394 - -0.5 63,394 - +0.7 63,394 - +0.5 63,394 - +1.2 63,394 - -0.3 63,394 - -0.3 63,394 - -2.3 63,394 - +1.7 63,394 - +1.7 63,394 - -0.5 63,433 + +3.7 63,434 - -0.5 63,443 + +1.7 63,443 + +0.7 63,443 + +0.2 63,443 + +4.2 63,514 + Psyr_0064 0.12 +2.0 63,514 + Psyr_0064 0.12 +1.0 63,514 + Psyr_0064 0.12 +1.1 63,515 - Psyr_0064 0.12 -0.5 63,515 - Psyr_0064 0.12 +2.5 63,515 - Psyr_0064 0.12 -2.0 63,515 - Psyr_0064 0.12 -2.1 63,515 - Psyr_0064 0.12 +3.6 63,516 + Psyr_0064 0.12 -3.3 63,516 + Psyr_0064 0.12 +4.2 63,517 - Psyr_0064 0.12 -1.3 63,517 - Psyr_0064 0.12 +2.2 63,517 - Psyr_0064 0.12 +0.0 63,538 - Psyr_0064 0.14 -0.8 63,592 + Psyr_0064 0.19 +0.7 63,611 + Psyr_0064 0.21 +0.3 63,611 + Psyr_0064 0.21 +1.2 63,611 + Psyr_0064 0.21 -0.7 63,611 + Psyr_0064 0.21 +2.2 63,611 + Psyr_0064 0.21 +0.5 63,611 + Psyr_0064 0.21 -1.3 63,611 + Psyr_0064 0.21 -2.9 63,611 + Psyr_0064 0.21 -0.4 63,611 + Psyr_0064 0.21 +3.7 63,611 + Psyr_0064 0.21 -1.5 63,611 + Psyr_0064 0.21 +0.9 63,611 + Psyr_0064 0.21 -1.5 63,611 + Psyr_0064 0.21 +0.5 63,611 + Psyr_0064 0.21 +2.2 63,612 - Psyr_0064 0.21 +3.0 63,612 - Psyr_0064 0.21 +0.2 63,612 - Psyr_0064 0.21 +3.3 63,612 - Psyr_0064 0.21 +0.6 63,628 + Psyr_0064 0.22 +0.2 63,629 - Psyr_0064 0.22 +0.2 63,662 + Psyr_0064 0.25 +1.2 63,662 + Psyr_0064 0.25 -1.0 63,662 + Psyr_0064 0.25 -4.1 63,662 + Psyr_0064 0.25 -1.5 63,663 - Psyr_0064 0.25 -3.9 63,682 + Psyr_0064 0.27 -0.9 63,697 + Psyr_0064 0.29 +0.6 63,697 + Psyr_0064 0.29 +4.1 63,752 - Psyr_0064 0.34 -0.8 63,752 - Psyr_0064 0.34 -1.1 63,770 + Psyr_0064 0.35 +0.2 63,784 + Psyr_0064 0.37 +0.3 63,789 - Psyr_0064 0.37 +3.6 64,032 - Psyr_0064 0.59 +4.7 64,037 + Psyr_0064 0.60 +2.2 64,037 + Psyr_0064 0.60 -1.0 64,037 + Psyr_0064 0.60 +0.5 64,038 - Psyr_0064 0.60 -1.3 64,089 - -2.1 64,261 + Psyr_0064 0.81 -1.2 64,262 - Psyr_0064 0.81 +1.2 64,262 - Psyr_0064 0.81 +3.0 64,522 + +0.9 64,522 + -0.9 64,522 + -4.4 64,522 + -1.1 64,522 + -2.8 64,522 + -2.6 64,522 + +0.2 64,522 + -2.2 64,522 + +4.1 64,522 + +3.6 64,522 + -3.6 64,522 + -1.5 64,522 + +0.5 64,522 + -1.8 64,522 + +2.7 64,522 + +3.5 64,523 - -0.7 64,523 - +2.5 64,523 - -0.5 64,523 - -1.4 64,523 - -0.3 64,523 - -1.1 64,523 - +4.2 64,523 - +0.9 64,523 - -0.8 64,523 - -0.9 64,523 - +0.6 64,523 - +3.0 64,538 + +2.7 64,539 - +0.5 64,565 + +3.2
Or see this region's nucleotide sequence