Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_983 and Pf1N1B4_984 are separated by 35 nucleotides Pf1N1B4_984 and Pf1N1B4_985 are separated by 15 nucleotides
Pf1N1B4_983 - Alginate biosynthesis protein Alg8, at 1,111,632 to 1,113,113
_983
Pf1N1B4_984 - Alginate biosynthesis protein Alg44, at 1,113,149 to 1,114,318
_984
Pf1N1B4_985 - Alginate biosynthesis protein AlgK precursor, at 1,114,334 to 1,115,839
_985
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1112.304 kb on + strand, within Pf1N1B4_983 at 1112.304 kb on + strand, within Pf1N1B4_983 at 1112.562 kb on + strand at 1112.562 kb on + strand, within Pf1N1B4_983 at 1112.562 kb on + strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.669 kb on + strand, within Pf1N1B4_983 at 1112.743 kb on + strand, within Pf1N1B4_983 at 1112.823 kb on + strand, within Pf1N1B4_983 at 1112.858 kb on + strand, within Pf1N1B4_983 at 1112.858 kb on + strand, within Pf1N1B4_983 at 1112.859 kb on - strand, within Pf1N1B4_983 at 1112.916 kb on + strand, within Pf1N1B4_983 at 1112.916 kb on + strand, within Pf1N1B4_983 at 1112.917 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.952 kb on + strand, within Pf1N1B4_983 at 1112.952 kb on + strand at 1112.952 kb on + strand at 1112.953 kb on - strand, within Pf1N1B4_983 at 1112.953 kb on - strand, within Pf1N1B4_983 at 1113.131 kb on - strand at 1113.131 kb on - strand at 1113.148 kb on + strand at 1113.148 kb on + strand at 1113.174 kb on + strand at 1113.174 kb on + strand at 1113.174 kb on + strand at 1113.175 kb on - strand at 1113.175 kb on - strand at 1113.526 kb on - strand, within Pf1N1B4_984 at 1113.643 kb on + strand, within Pf1N1B4_984 at 1113.693 kb on - strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.702 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand at 1113.989 kb on + strand, within Pf1N1B4_984 at 1114.317 kb on - strand at 1114.317 kb on - strand at 1114.341 kb on + strand at 1114.419 kb on + strand at 1114.420 kb on - strand at 1114.463 kb on + strand at 1114.580 kb on + strand, within Pf1N1B4_985 at 1114.580 kb on + strand, within Pf1N1B4_985 at 1114.581 kb on - strand, within Pf1N1B4_985 at 1114.581 kb on - strand, within Pf1N1B4_985 at 1114.605 kb on - strand, within Pf1N1B4_985 at 1114.686 kb on - strand, within Pf1N1B4_985 at 1114.686 kb on - strand, within Pf1N1B4_985 at 1114.686 kb on - strand, within Pf1N1B4_985 at 1114.686 kb on - strand, within Pf1N1B4_985 at 1114.851 kb on - strand, within Pf1N1B4_985 at 1114.851 kb on - strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1115.039 kb on + strand at 1115.039 kb on + strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.279 kb on + strand, within Pf1N1B4_985
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,112,304 + Pf1N1B4_983 0.45 -0.7 1,112,304 + Pf1N1B4_983 0.45 -0.6 1,112,562 + +0.6 1,112,562 + Pf1N1B4_983 0.63 -1.3 1,112,562 + Pf1N1B4_983 0.63 -3.4 1,112,563 - Pf1N1B4_983 0.63 +1.1 1,112,563 - Pf1N1B4_983 0.63 +0.3 1,112,563 - Pf1N1B4_983 0.63 -1.5 1,112,563 - Pf1N1B4_983 0.63 -0.3 1,112,563 - Pf1N1B4_983 0.63 -0.4 1,112,669 + Pf1N1B4_983 0.70 -1.9 1,112,743 + Pf1N1B4_983 0.75 -1.7 1,112,823 + Pf1N1B4_983 0.80 +0.1 1,112,858 + Pf1N1B4_983 0.83 -0.7 1,112,858 + Pf1N1B4_983 0.83 -3.2 1,112,859 - Pf1N1B4_983 0.83 -1.3 1,112,916 + Pf1N1B4_983 0.87 +0.3 1,112,916 + Pf1N1B4_983 0.87 -1.0 1,112,917 - Pf1N1B4_983 0.87 -1.7 1,112,920 - Pf1N1B4_983 0.87 -0.3 1,112,920 - Pf1N1B4_983 0.87 -1.9 1,112,920 - Pf1N1B4_983 0.87 -0.9 1,112,920 - Pf1N1B4_983 0.87 -1.0 1,112,920 - Pf1N1B4_983 0.87 +0.6 1,112,952 + Pf1N1B4_983 0.89 -1.0 1,112,952 + +0.3 1,112,952 + -1.5 1,112,953 - Pf1N1B4_983 0.89 +0.8 1,112,953 - Pf1N1B4_983 0.89 -1.4 1,113,131 - +1.3 1,113,131 - -1.7 1,113,148 + -0.7 1,113,148 + -1.1 1,113,174 + -2.0 1,113,174 + -0.6 1,113,174 + -1.0 1,113,175 - -1.8 1,113,175 - -0.3 1,113,526 - Pf1N1B4_984 0.32 -1.7 1,113,643 + Pf1N1B4_984 0.42 -0.7 1,113,693 - Pf1N1B4_984 0.46 -2.0 1,113,701 + Pf1N1B4_984 0.47 -1.7 1,113,701 + Pf1N1B4_984 0.47 -1.3 1,113,701 + Pf1N1B4_984 0.47 -1.0 1,113,701 + Pf1N1B4_984 0.47 +1.7 1,113,701 + Pf1N1B4_984 0.47 -0.7 1,113,701 + Pf1N1B4_984 0.47 -1.0 1,113,702 - Pf1N1B4_984 0.47 -0.3 1,113,856 - Pf1N1B4_984 0.60 -1.0 1,113,856 - Pf1N1B4_984 0.60 -1.0 1,113,856 - -0.7 1,113,989 + Pf1N1B4_984 0.72 -1.3 1,114,317 - -1.0 1,114,317 - +0.6 1,114,341 + -1.7 1,114,419 + +3.1 1,114,420 - +0.3 1,114,463 + -1.8 1,114,580 + Pf1N1B4_985 0.16 -2.4 1,114,580 + Pf1N1B4_985 0.16 -0.5 1,114,581 - Pf1N1B4_985 0.16 +0.1 1,114,581 - Pf1N1B4_985 0.16 -0.0 1,114,605 - Pf1N1B4_985 0.18 +0.2 1,114,686 - Pf1N1B4_985 0.23 +1.5 1,114,686 - Pf1N1B4_985 0.23 +0.3 1,114,686 - Pf1N1B4_985 0.23 -0.7 1,114,686 - Pf1N1B4_985 0.23 -1.8 1,114,851 - Pf1N1B4_985 0.34 -0.3 1,114,851 - Pf1N1B4_985 0.34 +0.3 1,114,973 + Pf1N1B4_985 0.42 -1.8 1,114,973 + Pf1N1B4_985 0.42 -1.8 1,114,973 + Pf1N1B4_985 0.42 -1.0 1,114,974 - Pf1N1B4_985 0.42 +0.3 1,114,974 - Pf1N1B4_985 0.42 +0.3 1,114,974 - Pf1N1B4_985 0.42 -0.0 1,114,974 - Pf1N1B4_985 0.42 +0.6 1,115,039 + -1.7 1,115,039 + Pf1N1B4_985 0.47 -1.5 1,115,040 - Pf1N1B4_985 0.47 -0.3 1,115,040 - Pf1N1B4_985 0.47 -1.5 1,115,040 - Pf1N1B4_985 0.47 -0.7 1,115,279 + Pf1N1B4_985 0.63 -0.0
Or see this region's nucleotide sequence