Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_763 and Pf1N1B4_764 are separated by 115 nucleotides Pf1N1B4_764 and Pf1N1B4_765 are separated by 116 nucleotides
Pf1N1B4_763 - Ferric iron ABC transporter, ATP-binding protein, at 867,093 to 868,202
_763
Pf1N1B4_764 - Uncharacterized isomerase yddE, PhzC-PhzF family, at 868,318 to 869,100
_764
Pf1N1B4_765 - Cys-tRNA(Pro) deacylase YbaK, at 869,217 to 869,687
_765
Position (kb)
868
869
870 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 867.628 kb on + strand, within Pf1N1B4_763 at 867.628 kb on + strand, within Pf1N1B4_763 at 867.629 kb on - strand at 867.629 kb on - strand, within Pf1N1B4_763 at 867.629 kb on - strand, within Pf1N1B4_763 at 867.757 kb on - strand, within Pf1N1B4_763 at 867.765 kb on + strand, within Pf1N1B4_763 at 867.814 kb on - strand, within Pf1N1B4_763 at 867.832 kb on + strand, within Pf1N1B4_763 at 867.833 kb on - strand, within Pf1N1B4_763 at 867.872 kb on - strand, within Pf1N1B4_763 at 867.872 kb on - strand, within Pf1N1B4_763 at 867.888 kb on + strand, within Pf1N1B4_763 at 867.888 kb on + strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 867.889 kb on - strand, within Pf1N1B4_763 at 868.090 kb on + strand, within Pf1N1B4_763 at 868.249 kb on - strand at 868.274 kb on + strand at 868.275 kb on - strand at 868.275 kb on - strand at 868.275 kb on - strand at 868.330 kb on + strand at 868.331 kb on - strand at 868.331 kb on - strand at 868.331 kb on - strand at 868.331 kb on - strand at 868.427 kb on + strand, within Pf1N1B4_764 at 868.428 kb on - strand, within Pf1N1B4_764 at 868.654 kb on + strand, within Pf1N1B4_764 at 868.655 kb on - strand, within Pf1N1B4_764 at 868.844 kb on + strand, within Pf1N1B4_764 at 868.844 kb on + strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.845 kb on - strand, within Pf1N1B4_764 at 868.862 kb on + strand, within Pf1N1B4_764 at 868.863 kb on - strand, within Pf1N1B4_764 at 868.863 kb on - strand, within Pf1N1B4_764 at 868.863 kb on - strand, within Pf1N1B4_764 at 868.914 kb on + strand, within Pf1N1B4_764 at 869.092 kb on + strand at 869.093 kb on - strand at 869.093 kb on - strand at 869.093 kb on - strand at 869.183 kb on + strand at 869.184 kb on - strand at 869.184 kb on - strand at 869.184 kb on - strand at 869.184 kb on - strand at 869.184 kb on - strand at 869.184 kb on - strand at 869.355 kb on - strand, within Pf1N1B4_765 at 869.355 kb on - strand, within Pf1N1B4_765 at 869.602 kb on + strand, within Pf1N1B4_765 at 869.602 kb on + strand, within Pf1N1B4_765 at 869.602 kb on + strand, within Pf1N1B4_765 at 869.602 kb on + strand at 869.629 kb on + strand, within Pf1N1B4_765 at 869.629 kb on + strand, within Pf1N1B4_765 at 869.736 kb on - strand at 869.751 kb on - strand at 869.763 kb on + strand at 869.763 kb on + strand at 869.763 kb on + strand at 869.763 kb on + strand at 869.763 kb on + strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.764 kb on - strand at 869.771 kb on + strand at 869.772 kb on - strand at 869.855 kb on + strand at 869.936 kb on + strand at 869.936 kb on + strand at 869.936 kb on + strand at 870.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 867,628 + Pf1N1B4_763 0.48 +0.4 867,628 + Pf1N1B4_763 0.48 -0.8 867,629 - -1.8 867,629 - Pf1N1B4_763 0.48 +0.6 867,629 - Pf1N1B4_763 0.48 +1.7 867,757 - Pf1N1B4_763 0.60 -1.3 867,765 + Pf1N1B4_763 0.61 +0.9 867,814 - Pf1N1B4_763 0.65 -1.1 867,832 + Pf1N1B4_763 0.67 -1.1 867,833 - Pf1N1B4_763 0.67 -0.3 867,872 - Pf1N1B4_763 0.70 -2.5 867,872 - Pf1N1B4_763 0.70 -0.8 867,888 + Pf1N1B4_763 0.72 +0.7 867,888 + Pf1N1B4_763 0.72 +0.7 867,889 - Pf1N1B4_763 0.72 +0.2 867,889 - Pf1N1B4_763 0.72 -0.8 867,889 - Pf1N1B4_763 0.72 -0.1 868,090 + Pf1N1B4_763 0.90 -0.9 868,249 - +1.0 868,274 + +0.8 868,275 - +0.2 868,275 - +0.7 868,275 - -0.8 868,330 + -0.8 868,331 - -1.2 868,331 - +0.2 868,331 - -0.1 868,331 - -0.3 868,427 + Pf1N1B4_764 0.14 -0.5 868,428 - Pf1N1B4_764 0.14 -1.9 868,654 + Pf1N1B4_764 0.43 -0.6 868,655 - Pf1N1B4_764 0.43 +0.1 868,844 + Pf1N1B4_764 0.67 -1.1 868,844 + Pf1N1B4_764 0.67 -1.3 868,845 - Pf1N1B4_764 0.67 +2.2 868,845 - Pf1N1B4_764 0.67 +1.1 868,845 - Pf1N1B4_764 0.67 -1.8 868,845 - Pf1N1B4_764 0.67 -2.1 868,845 - Pf1N1B4_764 0.67 -0.1 868,845 - Pf1N1B4_764 0.67 -1.2 868,862 + Pf1N1B4_764 0.69 -1.1 868,863 - Pf1N1B4_764 0.70 -0.3 868,863 - Pf1N1B4_764 0.70 +1.2 868,863 - Pf1N1B4_764 0.70 -0.3 868,914 + Pf1N1B4_764 0.76 +1.2 869,092 + +0.8 869,093 - -2.5 869,093 - +1.2 869,093 - -0.3 869,183 + -0.1 869,184 - +1.2 869,184 - -1.2 869,184 - -1.9 869,184 - +0.2 869,184 - -1.0 869,184 - +2.0 869,355 - Pf1N1B4_765 0.29 -0.8 869,355 - Pf1N1B4_765 0.29 -0.9 869,602 + Pf1N1B4_765 0.82 -0.6 869,602 + Pf1N1B4_765 0.82 +2.8 869,602 + Pf1N1B4_765 0.82 +1.0 869,602 + -0.3 869,629 + Pf1N1B4_765 0.87 -2.1 869,629 + Pf1N1B4_765 0.87 -2.7 869,736 - +1.1 869,751 - +1.0 869,763 + -1.1 869,763 + -0.8 869,763 + +0.7 869,763 + -1.1 869,763 + -1.6 869,764 - +1.6 869,764 - +1.7 869,764 - +0.2 869,764 - +0.1 869,764 - -0.3 869,764 - +2.2 869,764 - +0.5 869,771 + -0.8 869,772 - +0.2 869,855 + -0.8 869,936 + -1.1 869,936 + +0.8 869,936 + -2.2 870,073 - -0.6
Or see this region's nucleotide sequence