Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_721 and Pf1N1B4_722 are separated by 4 nucleotides Pf1N1B4_722 and Pf1N1B4_723 are separated by 148 nucleotides Pf1N1B4_723 and Pf1N1B4_724 overlap by 11 nucleotides Pf1N1B4_724 and Pf1N1B4_725 are separated by 218 nucleotides
Pf1N1B4_721 - InaA protein, at 824,741 to 825,454
_721
Pf1N1B4_722 - SAM-dependent methyltransferases, at 825,459 to 826,127
_722
Pf1N1B4_723 - two-component system sensor protein, at 826,276 to 827,556
_723
Pf1N1B4_724 - DNA-binding response regulator ColR, at 827,546 to 828,229
_724
Pf1N1B4_725 - PAP2 superfamily protein, at 828,448 to 829,191
_725
Position (kb) 826
827
828 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 825.297 kb on + strand, within Pf1N1B4_721 at 825.298 kb on - strand, within Pf1N1B4_721 at 825.372 kb on - strand, within Pf1N1B4_721 at 825.400 kb on - strand at 825.461 kb on - strand at 825.461 kb on - strand at 825.471 kb on + strand at 825.472 kb on - strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.474 kb on + strand at 825.475 kb on - strand at 825.478 kb on + strand at 825.478 kb on + strand at 825.478 kb on + strand at 825.478 kb on + strand at 825.479 kb on - strand at 825.479 kb on - strand at 825.479 kb on - strand at 825.479 kb on - strand at 825.479 kb on - strand at 825.479 kb on - strand at 825.481 kb on - strand at 825.496 kb on + strand at 825.496 kb on + strand at 825.496 kb on + strand at 825.497 kb on - strand at 825.497 kb on - strand at 825.497 kb on - strand at 825.605 kb on + strand, within Pf1N1B4_722 at 825.822 kb on + strand, within Pf1N1B4_722 at 825.822 kb on + strand, within Pf1N1B4_722 at 826.066 kb on + strand at 826.067 kb on - strand at 826.090 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.164 kb on + strand at 826.165 kb on - strand at 826.165 kb on - strand at 826.165 kb on - strand at 826.165 kb on - strand at 826.165 kb on - strand at 826.169 kb on - strand at 826.216 kb on + strand at 826.216 kb on + strand at 826.216 kb on + strand at 826.217 kb on - strand at 826.472 kb on - strand, within Pf1N1B4_723 at 826.472 kb on - strand, within Pf1N1B4_723 at 826.472 kb on - strand, within Pf1N1B4_723 at 826.523 kb on + strand, within Pf1N1B4_723 at 826.523 kb on + strand, within Pf1N1B4_723 at 826.523 kb on + strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.524 kb on - strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.538 kb on + strand, within Pf1N1B4_723 at 826.539 kb on - strand, within Pf1N1B4_723 at 826.539 kb on - strand, within Pf1N1B4_723 at 826.539 kb on - strand, within Pf1N1B4_723 at 826.551 kb on + strand, within Pf1N1B4_723 at 826.551 kb on + strand, within Pf1N1B4_723 at 826.594 kb on - strand, within Pf1N1B4_723 at 826.594 kb on - strand, within Pf1N1B4_723 at 826.596 kb on - strand, within Pf1N1B4_723 at 826.675 kb on + strand, within Pf1N1B4_723 at 826.888 kb on + strand, within Pf1N1B4_723 at 826.888 kb on + strand, within Pf1N1B4_723 at 826.888 kb on + strand, within Pf1N1B4_723 at 826.889 kb on - strand, within Pf1N1B4_723 at 826.889 kb on - strand, within Pf1N1B4_723 at 826.889 kb on - strand, within Pf1N1B4_723 at 826.889 kb on - strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.000 kb on + strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.001 kb on - strand, within Pf1N1B4_723 at 827.029 kb on + strand, within Pf1N1B4_723 at 827.113 kb on + strand, within Pf1N1B4_723 at 827.114 kb on - strand, within Pf1N1B4_723 at 827.135 kb on - strand, within Pf1N1B4_723 at 827.135 kb on - strand, within Pf1N1B4_723 at 827.153 kb on - strand, within Pf1N1B4_723 at 827.210 kb on + strand, within Pf1N1B4_723 at 827.211 kb on - strand, within Pf1N1B4_723 at 827.246 kb on + strand, within Pf1N1B4_723 at 827.247 kb on - strand, within Pf1N1B4_723 at 827.247 kb on - strand, within Pf1N1B4_723 at 827.247 kb on - strand, within Pf1N1B4_723 at 827.308 kb on + strand, within Pf1N1B4_723 at 827.309 kb on - strand, within Pf1N1B4_723 at 827.339 kb on + strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.340 kb on - strand, within Pf1N1B4_723 at 827.547 kb on + strand at 827.548 kb on - strand at 827.723 kb on + strand, within Pf1N1B4_724 at 827.724 kb on - strand, within Pf1N1B4_724 at 827.724 kb on - strand, within Pf1N1B4_724 at 827.724 kb on - strand, within Pf1N1B4_724 at 827.724 kb on - strand, within Pf1N1B4_724 at 827.724 kb on - strand, within Pf1N1B4_724 at 827.827 kb on - strand, within Pf1N1B4_724 at 827.934 kb on + strand, within Pf1N1B4_724 at 828.174 kb on + strand at 828.244 kb on + strand at 828.252 kb on - strand at 828.276 kb on - strand at 828.316 kb on + strand at 828.317 kb on - strand at 828.317 kb on - strand at 828.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 825,297 + Pf1N1B4_721 0.78 -0.1 825,298 - Pf1N1B4_721 0.78 +0.5 825,372 - Pf1N1B4_721 0.88 -0.4 825,400 - -1.6 825,461 - -1.1 825,461 - -0.2 825,471 + +0.2 825,472 - -0.2 825,474 + +0.9 825,474 + +1.2 825,474 + +0.4 825,474 + +1.2 825,474 + -1.6 825,474 + +0.6 825,474 + +0.2 825,474 + -1.1 825,475 - -0.4 825,478 + +0.2 825,478 + -0.1 825,478 + -0.4 825,478 + -1.8 825,479 - -0.4 825,479 - -0.4 825,479 - -1.8 825,479 - +0.8 825,479 - -0.8 825,479 - -0.6 825,481 - -0.8 825,496 + -0.7 825,496 + +2.2 825,496 + -2.7 825,497 - -1.8 825,497 - -0.9 825,497 - -1.1 825,605 + Pf1N1B4_722 0.22 +1.7 825,822 + Pf1N1B4_722 0.54 +1.6 825,822 + Pf1N1B4_722 0.54 +0.0 826,066 + +0.7 826,067 - -0.6 826,090 + +1.0 826,164 + +0.2 826,164 + -1.8 826,164 + +0.8 826,164 + -1.2 826,164 + +0.4 826,164 + +1.8 826,164 + +5.3 826,164 + -0.0 826,164 + +0.2 826,164 + -1.6 826,164 + -0.0 826,164 + -1.6 826,164 + +0.6 826,164 + -0.2 826,164 + -0.9 826,164 + -0.8 826,165 - -2.0 826,165 - -1.8 826,165 - -1.4 826,165 - -1.9 826,165 - +0.1 826,169 - +0.2 826,216 + +1.0 826,216 + -2.1 826,216 + -1.6 826,217 - -1.1 826,472 - Pf1N1B4_723 0.15 -2.8 826,472 - Pf1N1B4_723 0.15 -2.2 826,472 - Pf1N1B4_723 0.15 -2.0 826,523 + Pf1N1B4_723 0.19 -1.5 826,523 + Pf1N1B4_723 0.19 -0.5 826,523 + Pf1N1B4_723 0.19 -0.1 826,524 - Pf1N1B4_723 0.19 -3.4 826,524 - Pf1N1B4_723 0.19 -2.1 826,524 - Pf1N1B4_723 0.19 +0.4 826,524 - Pf1N1B4_723 0.19 -2.0 826,524 - Pf1N1B4_723 0.19 +0.7 826,524 - Pf1N1B4_723 0.19 -1.8 826,524 - Pf1N1B4_723 0.19 -2.5 826,538 + Pf1N1B4_723 0.20 -0.1 826,538 + Pf1N1B4_723 0.20 +0.4 826,538 + Pf1N1B4_723 0.20 -1.0 826,538 + Pf1N1B4_723 0.20 -1.0 826,538 + Pf1N1B4_723 0.20 -3.0 826,538 + Pf1N1B4_723 0.20 +1.2 826,538 + Pf1N1B4_723 0.20 -1.1 826,539 - Pf1N1B4_723 0.21 -1.0 826,539 - Pf1N1B4_723 0.21 -3.0 826,539 - Pf1N1B4_723 0.21 -3.0 826,551 + Pf1N1B4_723 0.21 +0.2 826,551 + Pf1N1B4_723 0.21 -0.8 826,594 - Pf1N1B4_723 0.25 +0.6 826,594 - Pf1N1B4_723 0.25 -1.1 826,596 - Pf1N1B4_723 0.25 -2.0 826,675 + Pf1N1B4_723 0.31 -1.1 826,888 + Pf1N1B4_723 0.48 -0.1 826,888 + Pf1N1B4_723 0.48 -1.6 826,888 + Pf1N1B4_723 0.48 +0.8 826,889 - Pf1N1B4_723 0.48 -1.6 826,889 - Pf1N1B4_723 0.48 -3.2 826,889 - Pf1N1B4_723 0.48 -2.2 826,889 - Pf1N1B4_723 0.48 -1.8 827,000 + Pf1N1B4_723 0.57 +2.2 827,000 + Pf1N1B4_723 0.57 -1.1 827,000 + Pf1N1B4_723 0.57 -2.4 827,000 + Pf1N1B4_723 0.57 -1.6 827,000 + Pf1N1B4_723 0.57 -1.1 827,000 + Pf1N1B4_723 0.57 -2.0 827,001 - Pf1N1B4_723 0.57 -2.5 827,001 - Pf1N1B4_723 0.57 -3.2 827,001 - Pf1N1B4_723 0.57 -3.1 827,001 - Pf1N1B4_723 0.57 -3.5 827,001 - Pf1N1B4_723 0.57 -1.8 827,001 - Pf1N1B4_723 0.57 -1.2 827,001 - Pf1N1B4_723 0.57 -3.4 827,001 - Pf1N1B4_723 0.57 -2.5 827,029 + Pf1N1B4_723 0.59 -2.7 827,113 + Pf1N1B4_723 0.65 -2.2 827,114 - Pf1N1B4_723 0.65 -2.2 827,135 - Pf1N1B4_723 0.67 -1.8 827,135 - Pf1N1B4_723 0.67 -2.7 827,153 - Pf1N1B4_723 0.68 -2.5 827,210 + Pf1N1B4_723 0.73 -1.0 827,211 - Pf1N1B4_723 0.73 -4.3 827,246 + Pf1N1B4_723 0.76 +0.3 827,247 - Pf1N1B4_723 0.76 +0.2 827,247 - Pf1N1B4_723 0.76 -3.0 827,247 - Pf1N1B4_723 0.76 -2.4 827,308 + Pf1N1B4_723 0.81 -1.9 827,309 - Pf1N1B4_723 0.81 -0.4 827,339 + Pf1N1B4_723 0.83 -2.2 827,340 - Pf1N1B4_723 0.83 -3.4 827,340 - Pf1N1B4_723 0.83 -3.6 827,340 - Pf1N1B4_723 0.83 -1.6 827,340 - Pf1N1B4_723 0.83 -2.1 827,340 - Pf1N1B4_723 0.83 +0.4 827,340 - Pf1N1B4_723 0.83 -1.8 827,547 + -0.8 827,548 - -1.1 827,723 + Pf1N1B4_724 0.26 -1.1 827,724 - Pf1N1B4_724 0.26 +1.9 827,724 - Pf1N1B4_724 0.26 +1.4 827,724 - Pf1N1B4_724 0.26 -2.9 827,724 - Pf1N1B4_724 0.26 +3.8 827,724 - Pf1N1B4_724 0.26 -1.1 827,827 - Pf1N1B4_724 0.41 +1.2 827,934 + Pf1N1B4_724 0.57 +1.4 828,174 + +1.4 828,244 + -0.6 828,252 - -1.5 828,276 - -0.8 828,316 + -2.0 828,317 - -1.1 828,317 - +0.9 828,497 + -0.6
Or see this region's nucleotide sequence