Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_688 and Pf1N1B4_689 are separated by 41 nucleotides Pf1N1B4_689 and Pf1N1B4_690 are separated by 427 nucleotides Pf1N1B4_690 and Pf1N1B4_691 are separated by 129 nucleotides
Pf1N1B4_688 - FIG00955483: hypothetical protein, at 787,083 to 787,313
_688
Pf1N1B4_689 - FIG00954888: hypothetical protein, at 787,355 to 787,708
_689
Pf1N1B4_690 - Transcriptional regulator, AraC family, at 788,136 to 789,065
_690
Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250
_691
Position (kb) 788
789
790 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.269 kb on + strand, within Pf1N1B4_688 at 787.373 kb on + strand at 787.374 kb on - strand at 787.500 kb on + strand, within Pf1N1B4_689 at 787.500 kb on + strand, within Pf1N1B4_689 at 787.500 kb on + strand, within Pf1N1B4_689 at 787.501 kb on - strand, within Pf1N1B4_689 at 787.681 kb on + strand at 787.682 kb on - strand at 787.682 kb on - strand at 787.702 kb on + strand at 787.703 kb on - strand at 787.870 kb on + strand at 787.870 kb on + strand at 788.095 kb on - strand at 788.220 kb on - strand at 788.241 kb on + strand, within Pf1N1B4_690 at 788.241 kb on + strand, within Pf1N1B4_690 at 788.242 kb on - strand at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.370 kb on + strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.379 kb on + strand, within Pf1N1B4_690 at 788.380 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.563 kb on - strand, within Pf1N1B4_690 at 788.781 kb on + strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.886 kb on + strand, within Pf1N1B4_690 at 789.003 kb on + strand at 789.003 kb on + strand at 789.130 kb on + strand at 789.130 kb on + strand at 789.131 kb on - strand at 789.282 kb on - strand at 789.328 kb on - strand, within Pf1N1B4_691 at 789.328 kb on - strand, within Pf1N1B4_691 at 789.552 kb on - strand, within Pf1N1B4_691 at 789.552 kb on - strand, within Pf1N1B4_691 at 789.586 kb on + strand, within Pf1N1B4_691 at 789.587 kb on - strand, within Pf1N1B4_691 at 790.025 kb on + strand, within Pf1N1B4_691 at 790.044 kb on - strand, within Pf1N1B4_691 at 790.044 kb on - strand, within Pf1N1B4_691
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 787,269 + Pf1N1B4_688 0.81 +0.1 787,269 + Pf1N1B4_688 0.81 +1.9 787,269 + Pf1N1B4_688 0.81 -0.4 787,269 + Pf1N1B4_688 0.81 -2.3 787,269 + Pf1N1B4_688 0.81 -0.6 787,373 + +1.4 787,374 - +1.9 787,500 + Pf1N1B4_689 0.41 +0.6 787,500 + Pf1N1B4_689 0.41 -0.8 787,500 + Pf1N1B4_689 0.41 +0.0 787,501 - Pf1N1B4_689 0.41 -0.1 787,681 + -0.9 787,682 - -0.7 787,682 - +1.4 787,702 + -1.8 787,703 - -1.4 787,870 + -0.4 787,870 + -0.8 788,095 - +0.4 788,220 - +1.8 788,241 + Pf1N1B4_690 0.11 +0.5 788,241 + Pf1N1B4_690 0.11 +1.8 788,242 - -1.1 788,242 - Pf1N1B4_690 0.11 +1.0 788,242 - Pf1N1B4_690 0.11 +2.1 788,242 - Pf1N1B4_690 0.11 -1.4 788,242 - Pf1N1B4_690 0.11 +1.4 788,370 + Pf1N1B4_690 0.25 +0.6 788,371 - Pf1N1B4_690 0.25 +1.2 788,371 - Pf1N1B4_690 0.25 +1.0 788,371 - Pf1N1B4_690 0.25 +0.4 788,371 - Pf1N1B4_690 0.25 -0.9 788,371 - Pf1N1B4_690 0.25 +0.2 788,371 - Pf1N1B4_690 0.25 +0.4 788,379 + Pf1N1B4_690 0.26 +0.2 788,380 - Pf1N1B4_690 0.26 +0.2 788,395 - Pf1N1B4_690 0.28 -0.0 788,395 - Pf1N1B4_690 0.28 +0.8 788,395 - Pf1N1B4_690 0.28 +0.8 788,438 - Pf1N1B4_690 0.32 +1.7 788,438 - Pf1N1B4_690 0.32 +1.0 788,438 - Pf1N1B4_690 0.32 +0.9 788,438 - Pf1N1B4_690 0.32 +2.6 788,562 + Pf1N1B4_690 0.46 +0.5 788,562 + Pf1N1B4_690 0.46 -1.6 788,562 + Pf1N1B4_690 0.46 -2.8 788,562 + Pf1N1B4_690 0.46 -2.0 788,563 - Pf1N1B4_690 0.46 +1.0 788,781 + Pf1N1B4_690 0.69 +0.8 788,782 - Pf1N1B4_690 0.69 +0.6 788,782 - Pf1N1B4_690 0.69 -0.5 788,782 - Pf1N1B4_690 0.69 +0.6 788,886 + Pf1N1B4_690 0.81 -0.5 789,003 + -1.3 789,003 + +1.6 789,130 + -1.4 789,130 + +1.2 789,131 - +3.2 789,282 - -0.8 789,328 - Pf1N1B4_691 0.13 -1.1 789,328 - Pf1N1B4_691 0.13 +0.2 789,552 - Pf1N1B4_691 0.34 -1.6 789,552 - Pf1N1B4_691 0.34 +0.8 789,586 + Pf1N1B4_691 0.37 +1.4 789,587 - Pf1N1B4_691 0.37 +0.1 790,025 + Pf1N1B4_691 0.79 -1.4 790,044 - Pf1N1B4_691 0.80 +2.2 790,044 - Pf1N1B4_691 0.80 -0.6
Or see this region's nucleotide sequence