Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_641 and Pf1N1B4_642 are separated by 122 nucleotides Pf1N1B4_642 and Pf1N1B4_643 are separated by 100 nucleotides Pf1N1B4_643 and Pf1N1B4_644 are separated by 464 nucleotides
Pf1N1B4_641 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 736,265 to 738,040
_641
Pf1N1B4_642 - Glycerate kinase (EC 2.7.1.31), at 738,163 to 738,420
_642
Pf1N1B4_643 - FIG00955846: hypothetical protein, at 738,521 to 738,859
_643
Pf1N1B4_644 - Cold shock protein CspC, at 739,324 to 739,926
_644
Position (kb) 738
739 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 738.108 kb on - strand at 738.108 kb on - strand at 738.165 kb on - strand at 738.165 kb on - strand at 738.274 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.375 kb on - strand, within Pf1N1B4_642 at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.449 kb on - strand at 738.451 kb on - strand at 738.458 kb on - strand at 738.458 kb on - strand at 738.458 kb on - strand at 738.458 kb on - strand at 738.479 kb on - strand at 738.485 kb on - strand at 738.485 kb on - strand at 738.485 kb on - strand at 738.679 kb on + strand, within Pf1N1B4_643 at 738.680 kb on - strand, within Pf1N1B4_643 at 738.691 kb on + strand, within Pf1N1B4_643 at 738.699 kb on + strand, within Pf1N1B4_643 at 738.699 kb on + strand at 738.700 kb on - strand, within Pf1N1B4_643 at 738.700 kb on - strand, within Pf1N1B4_643 at 738.700 kb on - strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.806 kb on + strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.807 kb on - strand, within Pf1N1B4_643 at 738.853 kb on - strand at 738.853 kb on - strand at 738.853 kb on - strand at 738.859 kb on + strand at 738.860 kb on - strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.892 kb on + strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.893 kb on - strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.894 kb on + strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.895 kb on - strand at 738.897 kb on - strand at 738.897 kb on - strand at 738.941 kb on - strand at 738.941 kb on - strand at 738.954 kb on + strand at 738.954 kb on + strand at 738.954 kb on + strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.968 kb on + strand at 738.968 kb on + strand at 738.968 kb on + strand at 738.968 kb on + strand at 738.968 kb on + strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.969 kb on - strand at 738.974 kb on - strand at 739.011 kb on - strand at 739.033 kb on + strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.034 kb on - strand at 739.181 kb on - strand at 739.223 kb on - strand at 739.347 kb on - strand at 739.409 kb on - strand, within Pf1N1B4_644
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 738,108 - +1.5 738,108 - +0.7 738,165 - -0.3 738,165 - -0.8 738,274 - Pf1N1B4_642 0.43 +1.6 738,375 - Pf1N1B4_642 0.82 -1.8 738,375 - Pf1N1B4_642 0.82 -0.2 738,375 - Pf1N1B4_642 0.82 -2.7 738,375 - Pf1N1B4_642 0.82 -0.4 738,375 - Pf1N1B4_642 0.82 +1.9 738,375 - Pf1N1B4_642 0.82 +0.9 738,375 - Pf1N1B4_642 0.82 +0.1 738,375 - Pf1N1B4_642 0.82 -0.4 738,375 - Pf1N1B4_642 0.82 -0.1 738,375 - Pf1N1B4_642 0.82 -0.5 738,375 - +0.1 738,375 - Pf1N1B4_642 0.82 -1.7 738,375 - Pf1N1B4_642 0.82 -1.5 738,375 - Pf1N1B4_642 0.82 -1.0 738,375 - Pf1N1B4_642 0.82 -2.2 738,449 - -0.4 738,449 - +1.0 738,449 - +0.0 738,449 - +0.5 738,449 - +0.8 738,449 - +1.6 738,449 - +1.7 738,449 - -2.2 738,449 - +0.1 738,449 - -1.9 738,449 - +0.3 738,449 - -0.6 738,449 - +0.7 738,451 - -0.7 738,458 - +1.9 738,458 - -0.2 738,458 - +0.2 738,458 - +0.3 738,479 - +0.1 738,485 - -1.9 738,485 - +2.1 738,485 - -0.9 738,679 + Pf1N1B4_643 0.47 +1.5 738,680 - Pf1N1B4_643 0.47 +0.6 738,691 + Pf1N1B4_643 0.50 -0.9 738,699 + Pf1N1B4_643 0.53 +0.1 738,699 + +1.7 738,700 - Pf1N1B4_643 0.53 -2.4 738,700 - Pf1N1B4_643 0.53 +0.7 738,700 - Pf1N1B4_643 0.53 +0.1 738,806 + Pf1N1B4_643 0.84 -0.9 738,806 + Pf1N1B4_643 0.84 +1.1 738,806 + Pf1N1B4_643 0.84 -1.2 738,806 + Pf1N1B4_643 0.84 -0.9 738,806 + Pf1N1B4_643 0.84 -1.1 738,806 + Pf1N1B4_643 0.84 -0.9 738,807 - Pf1N1B4_643 0.84 +1.1 738,807 - Pf1N1B4_643 0.84 +0.6 738,807 - Pf1N1B4_643 0.84 +1.0 738,807 - Pf1N1B4_643 0.84 -2.6 738,807 - Pf1N1B4_643 0.84 +1.0 738,807 - Pf1N1B4_643 0.84 -0.8 738,807 - Pf1N1B4_643 0.84 +1.6 738,807 - Pf1N1B4_643 0.84 -0.6 738,807 - Pf1N1B4_643 0.84 +0.2 738,807 - Pf1N1B4_643 0.84 +1.5 738,807 - Pf1N1B4_643 0.84 +2.1 738,807 - Pf1N1B4_643 0.84 -1.7 738,807 - Pf1N1B4_643 0.84 +0.8 738,807 - Pf1N1B4_643 0.84 +0.1 738,807 - Pf1N1B4_643 0.84 -1.0 738,807 - Pf1N1B4_643 0.84 -0.4 738,853 - +0.1 738,853 - -0.6 738,853 - -1.2 738,859 + -0.6 738,860 - +0.3 738,892 + -0.9 738,892 + -0.9 738,892 + -0.2 738,892 + +1.0 738,892 + -1.1 738,892 + -0.4 738,892 + +0.1 738,892 + +1.9 738,892 + +0.4 738,892 + -0.1 738,892 + +1.5 738,892 + +1.4 738,893 - +0.1 738,893 - -1.8 738,893 - -1.2 738,893 - -1.7 738,893 - +1.1 738,893 - -0.8 738,893 - -1.9 738,893 - -0.4 738,893 - -1.9 738,893 - +0.7 738,893 - +0.5 738,894 + -1.7 738,894 + +0.9 738,894 + -2.2 738,894 + +0.5 738,894 + +0.1 738,894 + -1.4 738,894 + -1.4 738,895 - -2.0 738,895 - +1.5 738,895 - +1.1 738,895 - +1.1 738,895 - -0.7 738,895 - +0.3 738,895 - -0.4 738,895 - +1.4 738,895 - -0.3 738,895 - -1.0 738,895 - -3.1 738,895 - -0.9 738,895 - -1.9 738,895 - -2.8 738,895 - -2.0 738,895 - +0.6 738,895 - -1.4 738,895 - +0.5 738,895 - +1.9 738,895 - +1.1 738,895 - +0.6 738,895 - -1.4 738,895 - -2.8 738,895 - +1.1 738,895 - -1.4 738,895 - -0.2 738,895 - -1.7 738,895 - -1.8 738,895 - +0.1 738,895 - +1.5 738,895 - -1.0 738,895 - -0.4 738,897 - -1.7 738,897 - +1.9 738,941 - +1.8 738,941 - -1.4 738,954 + +0.2 738,954 + -0.9 738,954 + +1.1 738,955 - +0.8 738,955 - -2.2 738,955 - -1.3 738,955 - -3.3 738,955 - -0.9 738,955 - +1.1 738,955 - +0.1 738,955 - +0.1 738,955 - -0.9 738,955 - -0.4 738,955 - +1.4 738,955 - +0.3 738,968 + -0.4 738,968 + +1.8 738,968 + +0.0 738,968 + +1.7 738,968 + +0.1 738,969 - +0.6 738,969 - +0.5 738,969 - +1.1 738,969 - -1.8 738,969 - -2.4 738,969 - -0.1 738,969 - +0.8 738,969 - +0.1 738,969 - +0.4 738,974 - +0.9 739,011 - -0.3 739,033 + +2.1 739,034 - -0.1 739,034 - -2.2 739,034 - -0.7 739,034 - -0.4 739,034 - +0.4 739,034 - -0.9 739,034 - -0.2 739,034 - -2.0 739,034 - -1.2 739,034 - +0.0 739,034 - -1.2 739,034 - -0.9 739,181 - -1.7 739,223 - -0.9 739,347 - -0.2 739,409 - Pf1N1B4_644 0.14 +1.1
Or see this region's nucleotide sequence