Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_635 and Pf1N1B4_636 overlap by 11 nucleotides Pf1N1B4_636 and Pf1N1B4_637 are separated by 127 nucleotides Pf1N1B4_637 and Pf1N1B4_638 are separated by 0 nucleotides Pf1N1B4_638 and Pf1N1B4_639 are separated by 115 nucleotides
Pf1N1B4_635 - MotA/TolQ/ExbB proton channel family protein, at 731,637 to 732,332
_635
Pf1N1B4_636 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 732,322 to 732,789
_636
Pf1N1B4_637 - Holliday junction DNA helicase RuvB, at 732,917 to 733,978
_637
Pf1N1B4_638 - Holliday junction DNA helicase RuvA, at 733,979 to 734,596
_638
Pf1N1B4_639 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 734,712 to 735,236
_639
Position (kb) 732
733
734 Strain fitness (log2 ratio) -2
-1
0
1
2 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.475 kb on - strand, within Pf1N1B4_636 at 732.533 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.696 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.795 kb on - strand at 732.795 kb on - strand at 732.802 kb on - strand at 732.802 kb on - strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.915 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.947 kb on + strand at 733.044 kb on + strand, within Pf1N1B4_637 at 733.062 kb on + strand, within Pf1N1B4_637 at 733.881 kb on + strand at 733.882 kb on - strand at 733.980 kb on + strand at 733.981 kb on - strand at 733.981 kb on - strand at 734.534 kb on + strand, within Pf1N1B4_638 at 734.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 732,392 - Pf1N1B4_636 0.15 -2.0 732,392 - Pf1N1B4_636 0.15 +1.1 732,392 - Pf1N1B4_636 0.15 -1.1 732,392 - Pf1N1B4_636 0.15 -0.0 732,475 - Pf1N1B4_636 0.33 +0.7 732,533 - Pf1N1B4_636 0.45 -0.9 732,685 - Pf1N1B4_636 0.78 -0.1 732,685 - Pf1N1B4_636 0.78 -0.4 732,696 - Pf1N1B4_636 0.80 -1.2 732,705 - Pf1N1B4_636 0.82 -2.0 732,705 - Pf1N1B4_636 0.82 +1.1 732,708 - Pf1N1B4_636 0.82 -0.1 732,708 - Pf1N1B4_636 0.82 +0.1 732,708 - Pf1N1B4_636 0.82 +0.3 732,708 - Pf1N1B4_636 0.82 -2.0 732,790 - +0.0 732,790 - +1.0 732,790 - -0.9 732,790 - +0.5 732,790 - -1.9 732,795 - -0.7 732,795 - +1.4 732,802 - -0.4 732,802 - -0.9 732,867 + -0.1 732,867 + +0.6 732,867 + -1.3 732,896 + +0.1 732,896 + +0.1 732,896 + +1.1 732,896 + -0.7 732,915 + +2.7 732,918 + -0.4 732,918 + +2.5 732,918 + +2.1 732,918 + +1.8 732,918 + +0.9 732,947 + +2.1 733,044 + Pf1N1B4_637 0.12 +0.1 733,062 + Pf1N1B4_637 0.14 +0.1 733,881 + -0.3 733,882 - +1.1 733,980 + -2.3 733,981 - -1.9 733,981 - -1.2 734,534 + Pf1N1B4_638 0.90 -1.2 734,711 - +1.5
Or see this region's nucleotide sequence