Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6012 and Pf1N1B4_6013 overlap by 4 nucleotides Pf1N1B4_6013 and Pf1N1B4_6014 overlap by 4 nucleotides Pf1N1B4_6014 and Pf1N1B4_6015 overlap by 4 nucleotides
Pf1N1B4_6012 - COG0419: ATPase involved in DNA repair, at 472,823 to 475,912
_6012
Pf1N1B4_6013 - hypothetical protein, at 475,909 to 476,808
_6013
Pf1N1B4_6014 - ATP-dependent DNA helicase RecQ, at 476,805 to 479,408
_6014
Pf1N1B4_6015 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 479,405 to 482,761
_6015
Position (kb) 476
477
478
479
480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 475.817 kb on + strand at 475.817 kb on + strand at 476.016 kb on + strand, within Pf1N1B4_6013 at 476.017 kb on - strand, within Pf1N1B4_6013 at 476.017 kb on - strand, within Pf1N1B4_6013 at 476.120 kb on - strand, within Pf1N1B4_6013 at 476.274 kb on - strand, within Pf1N1B4_6013 at 476.334 kb on + strand, within Pf1N1B4_6013 at 476.550 kb on + strand, within Pf1N1B4_6013 at 476.588 kb on - strand, within Pf1N1B4_6013 at 476.682 kb on + strand, within Pf1N1B4_6013 at 476.708 kb on - strand, within Pf1N1B4_6013 at 476.708 kb on - strand, within Pf1N1B4_6013 at 476.709 kb on + strand, within Pf1N1B4_6013 at 476.710 kb on - strand, within Pf1N1B4_6013 at 476.710 kb on - strand, within Pf1N1B4_6013 at 476.710 kb on - strand, within Pf1N1B4_6013 at 476.797 kb on + strand at 476.797 kb on + strand at 476.802 kb on + strand at 476.814 kb on + strand at 476.839 kb on + strand at 476.839 kb on + strand at 476.914 kb on - strand at 476.917 kb on - strand at 476.917 kb on - strand at 476.917 kb on - strand at 476.917 kb on - strand at 476.949 kb on + strand at 476.949 kb on + strand at 476.950 kb on - strand at 476.950 kb on - strand at 476.950 kb on - strand at 476.970 kb on - strand at 477.028 kb on - strand at 477.042 kb on + strand at 477.043 kb on - strand at 477.071 kb on - strand, within Pf1N1B4_6014 at 477.211 kb on + strand, within Pf1N1B4_6014 at 477.220 kb on + strand, within Pf1N1B4_6014 at 477.220 kb on + strand at 477.220 kb on + strand, within Pf1N1B4_6014 at 477.221 kb on - strand, within Pf1N1B4_6014 at 477.414 kb on - strand, within Pf1N1B4_6014 at 477.504 kb on + strand, within Pf1N1B4_6014 at 477.504 kb on + strand, within Pf1N1B4_6014 at 477.504 kb on + strand, within Pf1N1B4_6014 at 477.578 kb on + strand, within Pf1N1B4_6014 at 477.578 kb on + strand at 477.579 kb on - strand, within Pf1N1B4_6014 at 477.579 kb on - strand, within Pf1N1B4_6014 at 477.579 kb on - strand, within Pf1N1B4_6014 at 477.579 kb on - strand, within Pf1N1B4_6014 at 477.579 kb on - strand at 477.579 kb on - strand, within Pf1N1B4_6014 at 477.725 kb on + strand, within Pf1N1B4_6014 at 477.725 kb on + strand, within Pf1N1B4_6014 at 477.726 kb on - strand, within Pf1N1B4_6014 at 477.726 kb on - strand, within Pf1N1B4_6014 at 477.835 kb on + strand, within Pf1N1B4_6014 at 477.835 kb on + strand, within Pf1N1B4_6014 at 477.835 kb on + strand, within Pf1N1B4_6014 at 477.835 kb on + strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand, within Pf1N1B4_6014 at 477.836 kb on - strand at 477.839 kb on + strand, within Pf1N1B4_6014 at 477.840 kb on - strand at 477.840 kb on - strand, within Pf1N1B4_6014 at 477.840 kb on - strand at 477.840 kb on - strand, within Pf1N1B4_6014 at 477.950 kb on + strand, within Pf1N1B4_6014 at 477.950 kb on + strand, within Pf1N1B4_6014 at 477.982 kb on + strand, within Pf1N1B4_6014 at 477.982 kb on + strand, within Pf1N1B4_6014 at 477.983 kb on - strand, within Pf1N1B4_6014 at 477.983 kb on - strand, within Pf1N1B4_6014 at 477.993 kb on + strand, within Pf1N1B4_6014 at 478.041 kb on - strand at 478.204 kb on - strand, within Pf1N1B4_6014 at 478.303 kb on + strand, within Pf1N1B4_6014 at 478.304 kb on - strand, within Pf1N1B4_6014 at 478.304 kb on - strand, within Pf1N1B4_6014 at 478.304 kb on - strand, within Pf1N1B4_6014 at 478.304 kb on - strand, within Pf1N1B4_6014 at 478.304 kb on - strand, within Pf1N1B4_6014 at 478.330 kb on - strand, within Pf1N1B4_6014 at 478.330 kb on - strand, within Pf1N1B4_6014 at 478.374 kb on + strand, within Pf1N1B4_6014 at 478.375 kb on - strand, within Pf1N1B4_6014 at 478.630 kb on - strand, within Pf1N1B4_6014 at 478.696 kb on + strand, within Pf1N1B4_6014 at 478.743 kb on + strand at 478.743 kb on + strand, within Pf1N1B4_6014 at 478.743 kb on + strand, within Pf1N1B4_6014 at 478.744 kb on - strand, within Pf1N1B4_6014 at 478.744 kb on - strand, within Pf1N1B4_6014 at 478.754 kb on + strand, within Pf1N1B4_6014 at 478.828 kb on - strand, within Pf1N1B4_6014 at 478.828 kb on - strand, within Pf1N1B4_6014 at 478.831 kb on - strand, within Pf1N1B4_6014 at 478.951 kb on + strand, within Pf1N1B4_6014 at 478.951 kb on + strand, within Pf1N1B4_6014 at 478.951 kb on + strand, within Pf1N1B4_6014 at 478.952 kb on - strand at 478.952 kb on - strand, within Pf1N1B4_6014 at 478.952 kb on - strand, within Pf1N1B4_6014 at 478.955 kb on + strand, within Pf1N1B4_6014 at 478.955 kb on + strand, within Pf1N1B4_6014 at 478.955 kb on + strand, within Pf1N1B4_6014 at 478.956 kb on - strand, within Pf1N1B4_6014 at 478.959 kb on - strand, within Pf1N1B4_6014 at 478.959 kb on - strand, within Pf1N1B4_6014 at 479.043 kb on + strand, within Pf1N1B4_6014 at 479.044 kb on - strand at 479.044 kb on - strand, within Pf1N1B4_6014 at 479.044 kb on - strand, within Pf1N1B4_6014 at 479.354 kb on + strand at 479.355 kb on - strand at 479.355 kb on - strand at 479.408 kb on + strand at 479.408 kb on + strand at 479.580 kb on - strand at 479.580 kb on - strand at 479.580 kb on - strand at 479.610 kb on + strand at 479.682 kb on - strand at 479.684 kb on + strand at 479.684 kb on + strand at 479.685 kb on - strand at 479.685 kb on - strand at 479.685 kb on - strand at 479.685 kb on - strand at 479.744 kb on - strand, within Pf1N1B4_6015 at 479.775 kb on + strand, within Pf1N1B4_6015 at 479.776 kb on - strand, within Pf1N1B4_6015 at 479.776 kb on - strand, within Pf1N1B4_6015 at 479.776 kb on - strand, within Pf1N1B4_6015 at 479.827 kb on - strand, within Pf1N1B4_6015 at 479.891 kb on + strand, within Pf1N1B4_6015 at 479.891 kb on + strand at 479.892 kb on - strand, within Pf1N1B4_6015 at 479.892 kb on - strand, within Pf1N1B4_6015 at 479.892 kb on - strand, within Pf1N1B4_6015 at 479.993 kb on - strand, within Pf1N1B4_6015 at 480.090 kb on + strand, within Pf1N1B4_6015 at 480.091 kb on - strand, within Pf1N1B4_6015 at 480.091 kb on - strand, within Pf1N1B4_6015 at 480.091 kb on - strand, within Pf1N1B4_6015 at 480.178 kb on + strand, within Pf1N1B4_6015 at 480.179 kb on - strand, within Pf1N1B4_6015 at 480.205 kb on - strand, within Pf1N1B4_6015 at 480.205 kb on - strand, within Pf1N1B4_6015 at 480.254 kb on + strand, within Pf1N1B4_6015 at 480.254 kb on + strand, within Pf1N1B4_6015 at 480.254 kb on + strand, within Pf1N1B4_6015 at 480.254 kb on + strand, within Pf1N1B4_6015 at 480.255 kb on - strand at 480.255 kb on - strand at 480.273 kb on + strand at 480.275 kb on + strand, within Pf1N1B4_6015 at 480.275 kb on + strand, within Pf1N1B4_6015 at 480.295 kb on + strand, within Pf1N1B4_6015 at 480.295 kb on + strand, within Pf1N1B4_6015 at 480.330 kb on + strand at 480.335 kb on + strand, within Pf1N1B4_6015 at 480.336 kb on - strand, within Pf1N1B4_6015 at 480.387 kb on - strand, within Pf1N1B4_6015 at 480.387 kb on - strand, within Pf1N1B4_6015
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 475,817 + +1.1 475,817 + -1.1 476,016 + Pf1N1B4_6013 0.12 -1.9 476,017 - Pf1N1B4_6013 0.12 +0.3 476,017 - Pf1N1B4_6013 0.12 -0.4 476,120 - Pf1N1B4_6013 0.23 -1.3 476,274 - Pf1N1B4_6013 0.41 +0.2 476,334 + Pf1N1B4_6013 0.47 -1.1 476,550 + Pf1N1B4_6013 0.71 -0.7 476,588 - Pf1N1B4_6013 0.75 -0.4 476,682 + Pf1N1B4_6013 0.86 +3.2 476,708 - Pf1N1B4_6013 0.89 -0.7 476,708 - Pf1N1B4_6013 0.89 -1.6 476,709 + Pf1N1B4_6013 0.89 +0.9 476,710 - Pf1N1B4_6013 0.89 +0.8 476,710 - Pf1N1B4_6013 0.89 -0.1 476,710 - Pf1N1B4_6013 0.89 -1.7 476,797 + +1.1 476,797 + -0.3 476,802 + -0.7 476,814 + -0.5 476,839 + -2.4 476,839 + -0.1 476,914 - -0.1 476,917 - -1.7 476,917 - -0.1 476,917 - +0.2 476,917 - +0.0 476,949 + -1.4 476,949 + -0.1 476,950 - -1.9 476,950 - -0.4 476,950 - +4.4 476,970 - -1.7 477,028 - +0.6 477,042 + -1.7 477,043 - -0.1 477,071 - Pf1N1B4_6014 0.10 +0.7 477,211 + Pf1N1B4_6014 0.16 -0.1 477,220 + Pf1N1B4_6014 0.16 -0.0 477,220 + -0.1 477,220 + Pf1N1B4_6014 0.16 -0.1 477,221 - Pf1N1B4_6014 0.16 -1.3 477,414 - Pf1N1B4_6014 0.23 +0.6 477,504 + Pf1N1B4_6014 0.27 +0.9 477,504 + Pf1N1B4_6014 0.27 +2.6 477,504 + Pf1N1B4_6014 0.27 -0.7 477,578 + Pf1N1B4_6014 0.30 +0.9 477,578 + +0.9 477,579 - Pf1N1B4_6014 0.30 +0.2 477,579 - Pf1N1B4_6014 0.30 -0.2 477,579 - Pf1N1B4_6014 0.30 -1.4 477,579 - Pf1N1B4_6014 0.30 +2.9 477,579 - +2.5 477,579 - Pf1N1B4_6014 0.30 +0.6 477,725 + Pf1N1B4_6014 0.35 -2.6 477,725 + Pf1N1B4_6014 0.35 +1.9 477,726 - Pf1N1B4_6014 0.35 -1.4 477,726 - Pf1N1B4_6014 0.35 -3.2 477,835 + Pf1N1B4_6014 0.40 -2.8 477,835 + Pf1N1B4_6014 0.40 +1.9 477,835 + Pf1N1B4_6014 0.40 -0.5 477,835 + Pf1N1B4_6014 0.40 +0.4 477,836 - Pf1N1B4_6014 0.40 -0.1 477,836 - Pf1N1B4_6014 0.40 -0.9 477,836 - Pf1N1B4_6014 0.40 -0.9 477,836 - Pf1N1B4_6014 0.40 +0.2 477,836 - Pf1N1B4_6014 0.40 -1.2 477,836 - Pf1N1B4_6014 0.40 -1.7 477,836 - Pf1N1B4_6014 0.40 -1.3 477,836 - Pf1N1B4_6014 0.40 -2.6 477,836 - +2.5 477,839 + Pf1N1B4_6014 0.40 -0.3 477,840 - -0.1 477,840 - Pf1N1B4_6014 0.40 +0.2 477,840 - -0.7 477,840 - Pf1N1B4_6014 0.40 -1.0 477,950 + Pf1N1B4_6014 0.44 -1.1 477,950 + Pf1N1B4_6014 0.44 +2.1 477,982 + Pf1N1B4_6014 0.45 +0.9 477,982 + Pf1N1B4_6014 0.45 -1.9 477,983 - Pf1N1B4_6014 0.45 -1.9 477,983 - Pf1N1B4_6014 0.45 +1.3 477,993 + Pf1N1B4_6014 0.46 +0.4 478,041 - -0.7 478,204 - Pf1N1B4_6014 0.54 -1.9 478,303 + Pf1N1B4_6014 0.58 +0.1 478,304 - Pf1N1B4_6014 0.58 -1.0 478,304 - Pf1N1B4_6014 0.58 -0.7 478,304 - Pf1N1B4_6014 0.58 +0.5 478,304 - Pf1N1B4_6014 0.58 -2.1 478,304 - Pf1N1B4_6014 0.58 -0.4 478,330 - Pf1N1B4_6014 0.59 +2.6 478,330 - Pf1N1B4_6014 0.59 +0.9 478,374 + Pf1N1B4_6014 0.60 -0.1 478,375 - Pf1N1B4_6014 0.60 -1.0 478,630 - Pf1N1B4_6014 0.70 +1.1 478,696 + Pf1N1B4_6014 0.73 -2.4 478,743 + -0.1 478,743 + Pf1N1B4_6014 0.74 +0.5 478,743 + Pf1N1B4_6014 0.74 +0.3 478,744 - Pf1N1B4_6014 0.74 +2.5 478,744 - Pf1N1B4_6014 0.74 +0.9 478,754 + Pf1N1B4_6014 0.75 +0.2 478,828 - Pf1N1B4_6014 0.78 -1.4 478,828 - Pf1N1B4_6014 0.78 -1.4 478,831 - Pf1N1B4_6014 0.78 -2.1 478,951 + Pf1N1B4_6014 0.82 +0.6 478,951 + Pf1N1B4_6014 0.82 +2.4 478,951 + Pf1N1B4_6014 0.82 +1.4 478,952 - -0.7 478,952 - Pf1N1B4_6014 0.82 -0.4 478,952 - Pf1N1B4_6014 0.82 -0.1 478,955 + Pf1N1B4_6014 0.83 -2.7 478,955 + Pf1N1B4_6014 0.83 -1.7 478,955 + Pf1N1B4_6014 0.83 -0.7 478,956 - Pf1N1B4_6014 0.83 +1.4 478,959 - Pf1N1B4_6014 0.83 -0.7 478,959 - Pf1N1B4_6014 0.83 -1.7 479,043 + Pf1N1B4_6014 0.86 +2.1 479,044 - +0.3 479,044 - Pf1N1B4_6014 0.86 +0.7 479,044 - Pf1N1B4_6014 0.86 -1.9 479,354 + -0.2 479,355 - -0.7 479,355 - +0.6 479,408 + +0.5 479,408 + -0.3 479,580 - -0.4 479,580 - -0.1 479,580 - +0.9 479,610 + -0.1 479,682 - -1.0 479,684 + +0.9 479,684 + -0.8 479,685 - -0.4 479,685 - +1.6 479,685 - -0.7 479,685 - -1.1 479,744 - Pf1N1B4_6015 0.10 -1.1 479,775 + Pf1N1B4_6015 0.11 -1.7 479,776 - Pf1N1B4_6015 0.11 +0.1 479,776 - Pf1N1B4_6015 0.11 +0.9 479,776 - Pf1N1B4_6015 0.11 +1.8 479,827 - Pf1N1B4_6015 0.13 -1.6 479,891 + Pf1N1B4_6015 0.14 -0.1 479,891 + -0.7 479,892 - Pf1N1B4_6015 0.15 -0.5 479,892 - Pf1N1B4_6015 0.15 -0.0 479,892 - Pf1N1B4_6015 0.15 -0.2 479,993 - Pf1N1B4_6015 0.18 -0.4 480,090 + Pf1N1B4_6015 0.20 -2.1 480,091 - Pf1N1B4_6015 0.20 +0.9 480,091 - Pf1N1B4_6015 0.20 -0.1 480,091 - Pf1N1B4_6015 0.20 +0.5 480,178 + Pf1N1B4_6015 0.23 -2.9 480,179 - Pf1N1B4_6015 0.23 +0.1 480,205 - Pf1N1B4_6015 0.24 +1.2 480,205 - Pf1N1B4_6015 0.24 -0.7 480,254 + Pf1N1B4_6015 0.25 -1.7 480,254 + Pf1N1B4_6015 0.25 -2.8 480,254 + Pf1N1B4_6015 0.25 +1.9 480,254 + Pf1N1B4_6015 0.25 +0.6 480,255 - -1.4 480,255 - -0.7 480,273 + -0.1 480,275 + Pf1N1B4_6015 0.26 -1.2 480,275 + Pf1N1B4_6015 0.26 -0.5 480,295 + Pf1N1B4_6015 0.27 -0.1 480,295 + Pf1N1B4_6015 0.27 -1.4 480,330 + -1.4 480,335 + Pf1N1B4_6015 0.28 -1.1 480,336 - Pf1N1B4_6015 0.28 +0.9 480,387 - Pf1N1B4_6015 0.29 -1.3 480,387 - Pf1N1B4_6015 0.29 -0.1
Or see this region's nucleotide sequence