Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5996 and Pf1N1B4_5997 are separated by 87 nucleotides Pf1N1B4_5997 and Pf1N1B4_5998 are separated by 14 nucleotides
Pf1N1B4_5996 - Transposase, at 447,126 to 448,178
_5996
Pf1N1B4_5997 - Taurine dioxygenase (EC 1.14.11.17), at 448,266 to 449,114
_5997
Pf1N1B4_5998 - Quinone oxidoreductase (EC 1.6.5.5), at 449,129 to 450,109
_5998
Position (kb) 448
449
450 Strain fitness (log2 ratio) -2
-1
0
1
2 at 447.344 kb on + strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.375 kb on - strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 448.027 kb on + strand, within Pf1N1B4_5996 at 448.111 kb on + strand at 448.184 kb on - strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.190 kb on - strand at 448.195 kb on + strand at 448.195 kb on + strand at 448.270 kb on + strand at 448.350 kb on - strand at 448.406 kb on - strand, within Pf1N1B4_5997 at 448.417 kb on + strand, within Pf1N1B4_5997 at 448.418 kb on - strand, within Pf1N1B4_5997 at 448.418 kb on - strand, within Pf1N1B4_5997 at 448.501 kb on + strand, within Pf1N1B4_5997 at 448.501 kb on + strand, within Pf1N1B4_5997 at 448.502 kb on - strand at 448.594 kb on + strand, within Pf1N1B4_5997 at 448.868 kb on - strand, within Pf1N1B4_5997 at 449.130 kb on + strand at 449.131 kb on - strand at 449.131 kb on - strand at 449.208 kb on + strand at 449.209 kb on - strand at 449.209 kb on - strand at 449.301 kb on + strand at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.302 kb on - strand, within Pf1N1B4_5998 at 449.532 kb on + strand at 449.532 kb on + strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.718 kb on + strand, within Pf1N1B4_5998 at 449.824 kb on - strand, within Pf1N1B4_5998 at 449.836 kb on + strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.853 kb on + strand at 449.854 kb on - strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.877 kb on - strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 447,344 + Pf1N1B4_5996 0.21 +0.9 447,345 - Pf1N1B4_5996 0.21 +0.5 447,345 - Pf1N1B4_5996 0.21 -2.3 447,375 - Pf1N1B4_5996 0.24 +0.9 447,544 + Pf1N1B4_5996 0.40 +1.5 447,544 + Pf1N1B4_5996 0.40 -1.8 447,544 + Pf1N1B4_5996 0.40 +1.1 447,545 - Pf1N1B4_5996 0.40 +0.1 447,545 - Pf1N1B4_5996 0.40 -1.4 448,027 + Pf1N1B4_5996 0.86 -0.3 448,111 + -1.1 448,184 - +0.9 448,189 + +1.2 448,189 + +0.7 448,189 + +0.9 448,189 + -0.1 448,190 - -1.9 448,195 + -1.4 448,195 + -0.1 448,270 + +0.2 448,350 - +0.5 448,406 - Pf1N1B4_5997 0.16 +1.0 448,417 + Pf1N1B4_5997 0.18 +0.7 448,418 - Pf1N1B4_5997 0.18 -1.4 448,418 - Pf1N1B4_5997 0.18 -0.7 448,501 + Pf1N1B4_5997 0.28 -0.3 448,501 + Pf1N1B4_5997 0.28 -0.2 448,502 - -1.1 448,594 + Pf1N1B4_5997 0.39 +1.0 448,868 - Pf1N1B4_5997 0.71 -1.1 449,130 + -0.5 449,131 - +0.5 449,131 - -1.1 449,208 + -1.1 449,209 - -1.4 449,209 - +1.9 449,301 + -1.1 449,301 + Pf1N1B4_5998 0.18 +0.8 449,301 + Pf1N1B4_5998 0.18 -1.7 449,301 + Pf1N1B4_5998 0.18 -2.3 449,302 - Pf1N1B4_5998 0.18 +1.6 449,532 + +0.9 449,532 + Pf1N1B4_5998 0.41 +1.2 449,533 - Pf1N1B4_5998 0.41 +1.4 449,533 - Pf1N1B4_5998 0.41 -1.4 449,533 - Pf1N1B4_5998 0.41 +0.2 449,533 - Pf1N1B4_5998 0.41 +0.3 449,718 + Pf1N1B4_5998 0.60 -2.4 449,824 - Pf1N1B4_5998 0.71 +0.2 449,836 + Pf1N1B4_5998 0.72 -1.3 449,837 - Pf1N1B4_5998 0.72 -1.1 449,837 - Pf1N1B4_5998 0.72 +0.4 449,853 + -0.4 449,854 - Pf1N1B4_5998 0.74 -1.0 449,876 + Pf1N1B4_5998 0.76 +1.2 449,876 + -0.7 449,876 + Pf1N1B4_5998 0.76 -1.1 449,876 + Pf1N1B4_5998 0.76 -1.4 449,876 + Pf1N1B4_5998 0.76 -0.8 449,876 + +0.9 449,877 - Pf1N1B4_5998 0.76 -1.4 449,973 + Pf1N1B4_5998 0.86 -1.4 449,973 + Pf1N1B4_5998 0.86 +0.7 449,974 - Pf1N1B4_5998 0.86 +0.6 449,974 - Pf1N1B4_5998 0.86 +0.5 449,974 - Pf1N1B4_5998 0.86 -1.7
Or see this region's nucleotide sequence