Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5947 and Pf1N1B4_5948 are separated by 780 nucleotides Pf1N1B4_5948 and Pf1N1B4_5949 are separated by 76 nucleotides Pf1N1B4_5949 and Pf1N1B4_5950 overlap by 4 nucleotides
Pf1N1B4_5947 - Low molecular weight protein-tyrosine-phosphatase Wzb (EC 3.1.3.48), at 396,695 to 397,012
_5947
Pf1N1B4_5948 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 397,793 to 398,626
_5948
Pf1N1B4_5949 - Phosphomannomutase (EC 5.4.2.8), at 398,703 to 399,134
_5949
Pf1N1B4_5950 - Transcriptional activator RfaH, at 399,131 to 399,637
_5950
Position (kb) 397
398
399 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 397.174 kb on - strand at 397.174 kb on - strand at 397.350 kb on - strand at 397.418 kb on + strand at 397.443 kb on - strand at 397.444 kb on + strand at 397.444 kb on + strand at 397.445 kb on - strand at 397.445 kb on - strand at 397.774 kb on + strand at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 398.101 kb on - strand, within Pf1N1B4_5948 at 398.214 kb on + strand, within Pf1N1B4_5948 at 398.238 kb on - strand, within Pf1N1B4_5948 at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.528 kb on + strand at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.529 kb on - strand, within Pf1N1B4_5948 at 398.573 kb on + strand at 398.573 kb on + strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.679 kb on + strand at 398.679 kb on + strand at 398.682 kb on + strand at 398.702 kb on + strand at 398.762 kb on + strand, within Pf1N1B4_5949 at 398.763 kb on - strand, within Pf1N1B4_5949 at 398.778 kb on + strand, within Pf1N1B4_5949
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 397,174 - +1.2 397,174 - +2.5 397,350 - -1.1 397,418 + +2.4 397,443 - +4.0 397,444 + +2.2 397,444 + -1.1 397,445 - -0.7 397,445 - +6.8 397,774 + +0.5 397,888 + Pf1N1B4_5948 0.11 +1.6 397,888 + Pf1N1B4_5948 0.11 +1.9 397,942 + Pf1N1B4_5948 0.18 +2.7 397,942 + Pf1N1B4_5948 0.18 +1.3 397,942 + Pf1N1B4_5948 0.18 +2.3 397,942 + +1.3 397,942 + Pf1N1B4_5948 0.18 +2.8 397,942 + Pf1N1B4_5948 0.18 +1.8 397,943 - Pf1N1B4_5948 0.18 +2.1 397,943 - Pf1N1B4_5948 0.18 +2.6 397,943 - Pf1N1B4_5948 0.18 +2.7 397,943 - Pf1N1B4_5948 0.18 -0.3 398,101 - Pf1N1B4_5948 0.37 +1.2 398,214 + Pf1N1B4_5948 0.50 +0.5 398,238 - Pf1N1B4_5948 0.53 +1.8 398,528 + Pf1N1B4_5948 0.88 +2.5 398,528 + +4.4 398,528 + Pf1N1B4_5948 0.88 +2.1 398,529 - Pf1N1B4_5948 0.88 +2.6 398,573 + +1.7 398,573 + +1.8 398,574 - -0.1 398,574 - +4.1 398,574 - +3.8 398,679 + -1.3 398,679 + +2.7 398,682 + +3.1 398,702 + +3.1 398,762 + Pf1N1B4_5949 0.14 +1.1 398,763 - Pf1N1B4_5949 0.14 +0.8 398,778 + Pf1N1B4_5949 0.17 +3.8
Or see this region's nucleotide sequence