Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5812 and Pf1N1B4_5813 are separated by 393 nucleotides Pf1N1B4_5813 and Pf1N1B4_5814 are separated by 60 nucleotides Pf1N1B4_5814 and Pf1N1B4_5815 are separated by 15 nucleotides
Pf1N1B4_5812 - FIG00955248: hypothetical protein, at 244,789 to 245,487
_5812
Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756
_5813
Pf1N1B4_5814 - putative membrane protein, at 246,817 to 247,203
_5814
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Position (kb) 245
246
247 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 245.067 kb on - strand, within Pf1N1B4_5812 at 245.094 kb on - strand, within Pf1N1B4_5812 at 245.094 kb on - strand, within Pf1N1B4_5812 at 245.211 kb on + strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.212 kb on - strand, within Pf1N1B4_5812 at 245.487 kb on + strand at 245.488 kb on - strand at 245.496 kb on - strand at 245.615 kb on - strand at 245.640 kb on + strand at 245.641 kb on - strand at 245.641 kb on - strand at 245.641 kb on - strand at 245.806 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.835 kb on - strand at 245.898 kb on + strand at 245.899 kb on - strand at 245.899 kb on - strand at 245.899 kb on - strand at 246.197 kb on - strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.458 kb on + strand, within Pf1N1B4_5813 at 246.582 kb on - strand, within Pf1N1B4_5813 at 246.755 kb on - strand at 246.797 kb on + strand at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.147 kb on + strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.265 kb on + strand at 247.265 kb on + strand at 247.266 kb on - strand at 247.734 kb on + strand, within Pf1N1B4_5815
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 245,067 - Pf1N1B4_5812 0.40 -3.8 245,094 - Pf1N1B4_5812 0.44 -0.1 245,094 - Pf1N1B4_5812 0.44 -2.3 245,211 + Pf1N1B4_5812 0.60 -0.1 245,212 - Pf1N1B4_5812 0.61 +2.9 245,212 - Pf1N1B4_5812 0.61 +1.4 245,212 - Pf1N1B4_5812 0.61 +1.0 245,212 - Pf1N1B4_5812 0.61 -0.7 245,487 + -0.7 245,488 - -0.1 245,496 - -1.1 245,615 - +0.9 245,640 + +1.5 245,641 - +1.6 245,641 - -0.7 245,641 - +0.9 245,806 + +0.4 245,834 + +0.9 245,834 + -0.1 245,834 + +0.4 245,834 + +0.2 245,834 + -1.1 245,835 - +3.1 245,898 + -1.0 245,899 - -1.7 245,899 - +0.6 245,899 - +0.8 246,197 - Pf1N1B4_5813 0.36 -0.9 246,199 + Pf1N1B4_5813 0.36 -1.5 246,199 + Pf1N1B4_5813 0.36 -1.1 246,458 + Pf1N1B4_5813 0.66 -1.9 246,582 - Pf1N1B4_5813 0.80 +1.9 246,755 - -0.6 246,797 + +0.7 247,057 + Pf1N1B4_5814 0.62 -0.0 247,057 + Pf1N1B4_5814 0.62 +0.9 247,058 - Pf1N1B4_5814 0.62 -0.7 247,058 - Pf1N1B4_5814 0.62 -0.7 247,147 + Pf1N1B4_5814 0.85 -1.1 247,148 - Pf1N1B4_5814 0.86 -0.1 247,148 - Pf1N1B4_5814 0.86 +0.2 247,265 + -0.6 247,265 + -1.7 247,266 - -1.5 247,734 + Pf1N1B4_5815 0.33 +1.9
Or see this region's nucleotide sequence