Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5772 and Pf1N1B4_5773 are separated by 70 nucleotides Pf1N1B4_5773 and Pf1N1B4_5774 overlap by 4 nucleotides Pf1N1B4_5774 and Pf1N1B4_5775 are separated by 433 nucleotides
Pf1N1B4_5772 - Heme d1 biosynthesis protein NirJ, at 208,778 to 209,956
_5772
Pf1N1B4_5773 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 210,027 to 210,866
_5773
Pf1N1B4_5774 - Nitrite reductase associated c-type cytochorome NirN, at 210,863 to 212,356
_5774
Pf1N1B4_5775 - hypothetical protein, at 212,790 to 213,143
_5775
Position (kb) 210
211
212
213 Strain fitness (log2 ratio) -2
-1
0
1 at 210.378 kb on + strand, within Pf1N1B4_5773 at 210.579 kb on + strand, within Pf1N1B4_5773 at 210.580 kb on - strand, within Pf1N1B4_5773 at 211.228 kb on + strand, within Pf1N1B4_5774 at 211.229 kb on - strand, within Pf1N1B4_5774 at 211.406 kb on + strand, within Pf1N1B4_5774 at 211.439 kb on + strand, within Pf1N1B4_5774 at 211.439 kb on + strand, within Pf1N1B4_5774 at 211.542 kb on - strand at 211.542 kb on - strand, within Pf1N1B4_5774 at 211.542 kb on - strand, within Pf1N1B4_5774 at 211.793 kb on + strand, within Pf1N1B4_5774 at 211.875 kb on + strand, within Pf1N1B4_5774 at 211.925 kb on - strand, within Pf1N1B4_5774 at 212.218 kb on + strand at 212.249 kb on + strand at 212.309 kb on + strand at 212.309 kb on + strand at 212.309 kb on + strand at 212.309 kb on + strand at 212.309 kb on + strand at 212.310 kb on - strand at 212.310 kb on - strand at 212.310 kb on - strand at 212.310 kb on - strand at 212.403 kb on + strand at 212.660 kb on + strand at 212.740 kb on - strand at 213.251 kb on + strand at 213.251 kb on + strand at 213.324 kb on - strand at 213.324 kb on - strand at 213.327 kb on - strand at 213.349 kb on - strand at 213.349 kb on - strand at 213.352 kb on + strand at 213.352 kb on + strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 210,378 + Pf1N1B4_5773 0.42 +0.7 210,579 + Pf1N1B4_5773 0.66 +0.9 210,580 - Pf1N1B4_5773 0.66 -0.5 211,228 + Pf1N1B4_5774 0.24 +0.6 211,229 - Pf1N1B4_5774 0.24 -2.1 211,406 + Pf1N1B4_5774 0.36 -1.9 211,439 + Pf1N1B4_5774 0.39 -1.5 211,439 + Pf1N1B4_5774 0.39 -2.1 211,542 - -0.1 211,542 - Pf1N1B4_5774 0.45 -1.1 211,542 - Pf1N1B4_5774 0.45 -0.2 211,793 + Pf1N1B4_5774 0.62 -2.3 211,875 + Pf1N1B4_5774 0.68 -0.1 211,925 - Pf1N1B4_5774 0.71 -0.9 212,218 + -0.7 212,249 + -0.6 212,309 + -1.1 212,309 + -1.5 212,309 + -1.5 212,309 + +0.5 212,309 + -0.1 212,310 - -1.1 212,310 - +0.9 212,310 - -0.4 212,310 - -1.6 212,403 + +0.3 212,660 + -1.5 212,740 - +0.9 213,251 + -1.7 213,251 + +0.1 213,324 - -1.5 213,324 - +0.5 213,327 - -0.5 213,349 - +0.5 213,349 - +0.1 213,352 + -1.9 213,352 + -1.7 213,353 - +1.5 213,353 - +0.9 213,353 - -0.5 213,353 - -0.7
Or see this region's nucleotide sequence