Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5718 and Pf1N1B4_5719 are separated by 354 nucleotides Pf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotides Pf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotides Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides
Pf1N1B4_5718 - Transcriptional regulator, Cro/CI family, at 161,223 to 162,014
_5718
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356
_5720
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Position (kb) 162
163
164 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 161.530 kb on + strand, within Pf1N1B4_5718 at 161.530 kb on + strand at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.696 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.833 kb on - strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.938 kb on - strand at 162.053 kb on + strand at 162.131 kb on - strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.298 kb on + strand at 162.298 kb on + strand at 162.304 kb on + strand at 162.305 kb on - strand at 162.305 kb on - strand at 162.355 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.391 kb on - strand at 162.413 kb on + strand at 162.413 kb on + strand at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand at 163.056 kb on + strand at 163.058 kb on + strand at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.177 kb on - strand, within Pf1N1B4_5720 at 163.394 kb on - strand at 163.394 kb on - strand at 163.394 kb on - strand at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 161,530 + Pf1N1B4_5718 0.39 +0.3 161,530 + +0.9 161,589 + Pf1N1B4_5718 0.46 +1.1 161,589 + Pf1N1B4_5718 0.46 +0.3 161,696 + Pf1N1B4_5718 0.60 +0.1 161,832 + Pf1N1B4_5718 0.77 -0.5 161,832 + Pf1N1B4_5718 0.77 +0.1 161,833 - -0.7 161,937 + -1.5 161,937 + -1.7 161,937 + +1.5 161,938 - +1.0 162,053 + -1.5 162,131 - -0.3 162,141 + -1.0 162,141 + -0.1 162,141 + -0.4 162,190 + -1.9 162,190 + -0.4 162,190 + -0.1 162,298 + +1.7 162,298 + +0.9 162,304 + -0.1 162,305 - -1.7 162,305 - +1.7 162,355 + +0.9 162,390 + -1.7 162,390 + +0.9 162,390 + -1.7 162,391 - -1.1 162,413 + -1.6 162,413 + +1.2 162,759 + Pf1N1B4_5719 0.58 -1.6 162,760 - Pf1N1B4_5719 0.58 -1.9 162,876 - Pf1N1B4_5719 0.75 -1.7 162,957 + Pf1N1B4_5719 0.87 +0.1 162,958 - -1.1 163,056 + +2.0 163,058 + +1.5 163,174 - Pf1N1B4_5720 0.26 +0.5 163,174 - Pf1N1B4_5720 0.26 -0.1 163,176 + Pf1N1B4_5720 0.26 -0.7 163,176 + Pf1N1B4_5720 0.26 +0.1 163,177 - Pf1N1B4_5720 0.27 -3.2 163,394 - -2.1 163,394 - -0.5 163,394 - +0.3 163,472 + -1.5 163,537 - +0.9 163,558 + -0.9 163,559 - -0.6 163,559 - -0.0 163,559 - +0.2 163,561 + -0.0 163,562 - -0.0 163,568 - +0.5 163,679 + Pf1N1B4_5721 0.76 -1.5 163,679 + Pf1N1B4_5721 0.76 -0.9 163,680 - Pf1N1B4_5721 0.77 +0.4 163,680 - Pf1N1B4_5721 0.77 -2.3 163,916 + Pf1N1B4_5722 0.54 -0.7 163,916 + Pf1N1B4_5722 0.54 +1.2 163,916 + Pf1N1B4_5722 0.54 +0.9 163,917 - Pf1N1B4_5722 0.54 -0.1 163,936 - Pf1N1B4_5722 0.59 +0.5
Or see this region's nucleotide sequence