Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5622

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5621 and Pf1N1B4_5622 are separated by 629 nucleotidesPf1N1B4_5622 and Pf1N1B4_5623 are separated by 58 nucleotides Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828 _5621 Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546 _5622 Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657 _5623 Position (kb) 48 49 50Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 47.766 kb on - strandat 47.891 kb on + strandat 47.958 kb on + strandat 47.958 kb on + strandat 48.084 kb on + strandat 48.084 kb on + strandat 48.140 kb on + strandat 48.140 kb on + strandat 48.262 kb on + strandat 48.262 kb on + strandat 48.271 kb on + strandat 48.271 kb on + strandat 48.272 kb on - strandat 48.272 kb on - strandat 48.272 kb on - strandat 48.307 kb on - strandat 48.559 kb on - strandat 48.761 kb on + strand, within Pf1N1B4_5622at 48.807 kb on - strand, within Pf1N1B4_5622at 49.205 kb on + strandat 49.206 kb on - strand, within Pf1N1B4_5622at 49.621 kb on - strandat 49.621 kb on - strandat 49.727 kb on + strand, within Pf1N1B4_5623at 49.728 kb on - strand, within Pf1N1B4_5623at 49.732 kb on - strand, within Pf1N1B4_5623at 49.732 kb on - strand, within Pf1N1B4_5623at 50.006 kb on - strand, within Pf1N1B4_5623at 50.006 kb on - strand, within Pf1N1B4_5623at 50.006 kb on - strand, within Pf1N1B4_5623at 50.043 kb on - strand, within Pf1N1B4_5623at 50.298 kb on + strand, within Pf1N1B4_5623at 50.339 kb on - strandat 50.348 kb on - strand, within Pf1N1B4_5623at 50.348 kb on - strandat 50.389 kb on - strand, within Pf1N1B4_5623at 50.389 kb on - strand, within Pf1N1B4_5623

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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47,766 - +2.1
47,891 + -0.2
47,958 + -1.5
47,958 + +2.2
48,084 + +0.6
48,084 + +1.1
48,140 + +2.0
48,140 + -0.5
48,262 + +1.3
48,262 + +0.1
48,271 + +0.3
48,271 + +1.0
48,272 - -0.7
48,272 - -0.5
48,272 - -1.5
48,307 - -0.1
48,559 - -1.9
48,761 + Pf1N1B4_5622 0.28 -1.8
48,807 - Pf1N1B4_5622 0.32 -0.7
49,205 + -0.1
49,206 - Pf1N1B4_5622 0.69 -1.5
49,621 - -0.6
49,621 - +0.1
49,727 + Pf1N1B4_5623 0.12 -2.3
49,728 - Pf1N1B4_5623 0.12 -1.1
49,732 - Pf1N1B4_5623 0.12 +2.3
49,732 - Pf1N1B4_5623 0.12 +3.9
50,006 - Pf1N1B4_5623 0.38 -0.2
50,006 - Pf1N1B4_5623 0.38 +0.4
50,006 - Pf1N1B4_5623 0.38 -1.5
50,043 - Pf1N1B4_5623 0.42 +0.3
50,298 + Pf1N1B4_5623 0.66 +0.5
50,339 - -0.7
50,348 - Pf1N1B4_5623 0.71 +0.8
50,348 - +1.3
50,389 - Pf1N1B4_5623 0.74 +1.3
50,389 - Pf1N1B4_5623 0.74 -1.1

Or see this region's nucleotide sequence