Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5621 and Pf1N1B4_5622 are separated by 629 nucleotides Pf1N1B4_5622 and Pf1N1B4_5623 are separated by 58 nucleotides
Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828
_5621
Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546
_5622
Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657
_5623
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 47.766 kb on - strand at 47.891 kb on + strand at 47.958 kb on + strand at 47.958 kb on + strand at 48.084 kb on + strand at 48.084 kb on + strand at 48.140 kb on + strand at 48.140 kb on + strand at 48.262 kb on + strand at 48.262 kb on + strand at 48.271 kb on + strand at 48.271 kb on + strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.307 kb on - strand at 48.559 kb on - strand at 48.761 kb on + strand, within Pf1N1B4_5622 at 48.807 kb on - strand, within Pf1N1B4_5622 at 49.205 kb on + strand at 49.206 kb on - strand, within Pf1N1B4_5622 at 49.621 kb on - strand at 49.621 kb on - strand at 49.727 kb on + strand, within Pf1N1B4_5623 at 49.728 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.043 kb on - strand, within Pf1N1B4_5623 at 50.298 kb on + strand, within Pf1N1B4_5623 at 50.339 kb on - strand at 50.348 kb on - strand, within Pf1N1B4_5623 at 50.348 kb on - strand at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.389 kb on - strand, within Pf1N1B4_5623
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 47,766 - +2.1 47,891 + -0.2 47,958 + -1.5 47,958 + +2.2 48,084 + +0.6 48,084 + +1.1 48,140 + +2.0 48,140 + -0.5 48,262 + +1.3 48,262 + +0.1 48,271 + +0.3 48,271 + +1.0 48,272 - -0.7 48,272 - -0.5 48,272 - -1.5 48,307 - -0.1 48,559 - -1.9 48,761 + Pf1N1B4_5622 0.28 -1.8 48,807 - Pf1N1B4_5622 0.32 -0.7 49,205 + -0.1 49,206 - Pf1N1B4_5622 0.69 -1.5 49,621 - -0.6 49,621 - +0.1 49,727 + Pf1N1B4_5623 0.12 -2.3 49,728 - Pf1N1B4_5623 0.12 -1.1 49,732 - Pf1N1B4_5623 0.12 +2.3 49,732 - Pf1N1B4_5623 0.12 +3.9 50,006 - Pf1N1B4_5623 0.38 -0.2 50,006 - Pf1N1B4_5623 0.38 +0.4 50,006 - Pf1N1B4_5623 0.38 -1.5 50,043 - Pf1N1B4_5623 0.42 +0.3 50,298 + Pf1N1B4_5623 0.66 +0.5 50,339 - -0.7 50,348 - Pf1N1B4_5623 0.71 +0.8 50,348 - +1.3 50,389 - Pf1N1B4_5623 0.74 +1.3 50,389 - Pf1N1B4_5623 0.74 -1.1
Or see this region's nucleotide sequence