Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5610 and Pf1N1B4_5611 are separated by 19 nucleotides Pf1N1B4_5611 and Pf1N1B4_5612 are separated by 37 nucleotides Pf1N1B4_5612 and Pf1N1B4_5613 are separated by 0 nucleotides
Pf1N1B4_5610 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 35,870 to 37,033
_5610
Pf1N1B4_5611 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 37,053 to 38,255
_5611
Pf1N1B4_5612 - Electron transfer flavoprotein, beta subunit, at 38,293 to 39,042
_5612
Pf1N1B4_5613 - Electron transfer flavoprotein, alpha subunit, at 39,043 to 39,999
_5613
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2 at 36.112 kb on + strand, within Pf1N1B4_5610 at 36.141 kb on - strand, within Pf1N1B4_5610 at 36.189 kb on - strand at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.313 kb on - strand, within Pf1N1B4_5610 at 36.330 kb on + strand, within Pf1N1B4_5610 at 36.330 kb on + strand at 36.579 kb on - strand, within Pf1N1B4_5610 at 36.839 kb on + strand, within Pf1N1B4_5610 at 36.840 kb on - strand, within Pf1N1B4_5610 at 36.992 kb on + strand at 36.993 kb on - strand at 36.993 kb on - strand at 36.993 kb on - strand at 37.031 kb on + strand at 37.032 kb on - strand at 37.032 kb on - strand at 37.590 kb on + strand at 37.591 kb on - strand, within Pf1N1B4_5611 at 37.705 kb on - strand, within Pf1N1B4_5611 at 37.708 kb on - strand, within Pf1N1B4_5611 at 38.270 kb on + strand at 38.306 kb on + strand at 38.432 kb on + strand, within Pf1N1B4_5612 at 38.432 kb on + strand, within Pf1N1B4_5612
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 36,112 + Pf1N1B4_5610 0.21 -0.9 36,141 - Pf1N1B4_5610 0.23 -0.1 36,189 - +1.9 36,192 - Pf1N1B4_5610 0.28 +0.6 36,192 - Pf1N1B4_5610 0.28 +0.4 36,308 + Pf1N1B4_5610 0.38 -1.1 36,308 + Pf1N1B4_5610 0.38 +0.6 36,313 - Pf1N1B4_5610 0.38 +0.0 36,330 + Pf1N1B4_5610 0.40 -0.1 36,330 + -0.1 36,579 - Pf1N1B4_5610 0.61 +0.5 36,839 + Pf1N1B4_5610 0.83 -1.1 36,840 - Pf1N1B4_5610 0.83 -0.5 36,992 + -0.5 36,993 - -0.8 36,993 - +0.1 36,993 - -1.5 37,031 + +0.3 37,032 - -0.4 37,032 - +0.1 37,590 + +0.9 37,591 - Pf1N1B4_5611 0.45 -1.1 37,705 - Pf1N1B4_5611 0.54 -1.6 37,708 - Pf1N1B4_5611 0.54 -1.5 38,270 + -0.9 38,306 + -0.1 38,432 + Pf1N1B4_5612 0.19 -0.1 38,432 + Pf1N1B4_5612 0.19 -1.9
Or see this region's nucleotide sequence