Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5606 and Pf1N1B4_5607 are separated by 557 nucleotides Pf1N1B4_5607 and Pf1N1B4_5608 are separated by 565 nucleotides
Pf1N1B4_5606 - Alpha-ketoglutarate permease, at 30,868 to 32,253
_5606
Pf1N1B4_5607 - C4-dicarboxylate transport protein, at 32,811 to 34,115
_5607
Pf1N1B4_5608 - Sulfopyruvate decarboxylase - alpha subunit (EC 4.1.1.79), at 34,681 to 35,220
_5608
Position (kb)
32
33
34
35 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 31.942 kb on + strand, within Pf1N1B4_5606 at 32.006 kb on + strand, within Pf1N1B4_5606 at 32.006 kb on + strand, within Pf1N1B4_5606 at 32.007 kb on - strand, within Pf1N1B4_5606 at 32.056 kb on + strand, within Pf1N1B4_5606 at 32.057 kb on - strand, within Pf1N1B4_5606 at 32.107 kb on + strand, within Pf1N1B4_5606 at 32.107 kb on + strand at 32.108 kb on - strand, within Pf1N1B4_5606 at 32.108 kb on - strand at 32.176 kb on + strand at 32.177 kb on - strand at 32.259 kb on - strand at 32.345 kb on - strand at 32.529 kb on - strand at 32.729 kb on + strand at 32.730 kb on - strand at 33.123 kb on - strand, within Pf1N1B4_5607 at 33.182 kb on - strand, within Pf1N1B4_5607 at 33.182 kb on - strand, within Pf1N1B4_5607 at 33.212 kb on - strand, within Pf1N1B4_5607 at 33.464 kb on - strand, within Pf1N1B4_5607 at 33.967 kb on - strand, within Pf1N1B4_5607 at 33.967 kb on - strand at 33.995 kb on - strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.329 kb on - strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.333 kb on - strand at 34.333 kb on - strand at 34.352 kb on + strand at 34.354 kb on + strand at 34.354 kb on + strand at 34.355 kb on - strand at 34.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 31,942 + Pf1N1B4_5606 0.77 +0.9 32,006 + Pf1N1B4_5606 0.82 +2.2 32,006 + Pf1N1B4_5606 0.82 -1.7 32,007 - Pf1N1B4_5606 0.82 -1.2 32,056 + Pf1N1B4_5606 0.86 -0.3 32,057 - Pf1N1B4_5606 0.86 +1.9 32,107 + Pf1N1B4_5606 0.89 +0.3 32,107 + -1.5 32,108 - Pf1N1B4_5606 0.89 -1.7 32,108 - +1.5 32,176 + -1.3 32,177 - -0.1 32,259 - -2.8 32,345 - -1.7 32,529 - +1.0 32,729 + -0.9 32,730 - -1.7 33,123 - Pf1N1B4_5607 0.24 -1.9 33,182 - Pf1N1B4_5607 0.28 -1.9 33,182 - Pf1N1B4_5607 0.28 +1.3 33,212 - Pf1N1B4_5607 0.31 -0.9 33,464 - Pf1N1B4_5607 0.50 -1.3 33,967 - Pf1N1B4_5607 0.89 -1.5 33,967 - -0.1 33,995 - +0.3 34,328 + -1.9 34,328 + -1.5 34,328 + +0.9 34,328 + +0.9 34,329 - -0.7 34,332 + -1.1 34,332 + -2.1 34,332 + -1.1 34,332 + -0.7 34,332 + -1.1 34,332 + +0.2 34,332 + -0.1 34,332 + -1.1 34,332 + +0.9 34,332 + +1.1 34,332 + +0.9 34,332 + +0.9 34,333 - -0.1 34,333 - +0.3 34,352 + -1.9 34,354 + +0.0 34,354 + -1.3 34,355 - -0.7 34,403 - -0.3
Or see this region's nucleotide sequence