Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5495 and Pf1N1B4_5496 are separated by 68 nucleotides
Pf1N1B4_5495 - Cyclic AMP receptor protein, at 243,236 to 243,814
_5495
Pf1N1B4_5496 - hypothetical protein, at 243,883 to 244,107
_5496
Position (kb) 243
244 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 242.278 kb on + strand at 242.329 kb on + strand at 242.329 kb on + strand at 242.506 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.622 kb on - strand at 242.790 kb on + strand at 242.790 kb on + strand at 242.791 kb on - strand at 242.817 kb on + strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.832 kb on - strand at 242.870 kb on + strand at 242.871 kb on - strand at 242.871 kb on - strand at 243.050 kb on - strand at 243.064 kb on + strand at 243.065 kb on - strand at 243.065 kb on - strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.155 kb on - strand at 243.292 kb on + strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.360 kb on + strand, within Pf1N1B4_5495 at 243.361 kb on - strand, within Pf1N1B4_5495 at 243.369 kb on + strand at 243.370 kb on - strand, within Pf1N1B4_5495 at 243.475 kb on - strand, within Pf1N1B4_5495 at 243.583 kb on + strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.719 kb on - strand, within Pf1N1B4_5495 at 243.790 kb on + strand at 243.791 kb on - strand at 243.791 kb on - strand at 243.834 kb on + strand at 243.834 kb on + strand at 243.835 kb on - strand at 243.835 kb on - strand at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 244.000 kb on + strand, within Pf1N1B4_5496 at 244.001 kb on - strand, within Pf1N1B4_5496 at 244.106 kb on - strand at 244.129 kb on - strand at 244.129 kb on - strand at 244.154 kb on + strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.300 kb on + strand at 244.431 kb on - strand at 244.431 kb on - strand at 244.445 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 242,278 + -0.5 242,329 + +3.4 242,329 + -1.2 242,506 + +0.2 242,520 + -1.2 242,520 + +1.0 242,520 + +0.8 242,521 - -2.2 242,521 - +2.6 242,521 - -1.0 242,521 - -0.8 242,521 - -1.2 242,521 - -1.8 242,521 - -1.2 242,622 - +1.6 242,790 + +0.3 242,790 + -2.0 242,791 - +0.3 242,817 + -0.2 242,818 - -0.1 242,818 - +1.8 242,818 - +1.6 242,818 - +1.2 242,831 + -1.8 242,831 + +1.9 242,831 + -1.8 242,832 - +0.8 242,870 + -3.5 242,871 - -0.5 242,871 - -2.5 243,050 - +0.6 243,064 + -0.6 243,065 - +0.1 243,065 - -0.5 243,081 + +0.8 243,081 + +0.0 243,081 + -0.2 243,082 - +0.0 243,082 - +0.4 243,082 - +0.9 243,082 - +0.6 243,082 - +0.1 243,082 - +1.7 243,155 - +1.3 243,292 + +2.1 243,293 - -1.4 243,293 - +1.4 243,293 - -1.2 243,296 + Pf1N1B4_5495 0.10 -2.0 243,296 + Pf1N1B4_5495 0.10 -1.8 243,296 + Pf1N1B4_5495 0.10 +0.0 243,296 + Pf1N1B4_5495 0.10 +1.2 243,296 + Pf1N1B4_5495 0.10 -0.2 243,297 - Pf1N1B4_5495 0.11 -1.8 243,297 - Pf1N1B4_5495 0.11 +0.4 243,297 - Pf1N1B4_5495 0.11 +1.6 243,351 + Pf1N1B4_5495 0.20 -0.2 243,351 + Pf1N1B4_5495 0.20 +1.4 243,351 + Pf1N1B4_5495 0.20 +1.0 243,351 + Pf1N1B4_5495 0.20 +2.5 243,352 - Pf1N1B4_5495 0.20 +0.5 243,352 - Pf1N1B4_5495 0.20 +0.5 243,360 + Pf1N1B4_5495 0.21 +0.8 243,361 - Pf1N1B4_5495 0.22 +0.7 243,369 + +2.4 243,370 - Pf1N1B4_5495 0.23 +1.8 243,475 - Pf1N1B4_5495 0.41 -2.7 243,583 + Pf1N1B4_5495 0.60 +0.8 243,584 - Pf1N1B4_5495 0.60 -1.8 243,584 - Pf1N1B4_5495 0.60 -3.2 243,719 - Pf1N1B4_5495 0.83 -2.2 243,790 + -1.5 243,791 - +1.8 243,791 - -1.5 243,834 + -1.2 243,834 + -2.0 243,835 - +0.8 243,835 - -2.4 243,922 + Pf1N1B4_5496 0.17 +1.4 243,922 + Pf1N1B4_5496 0.17 -0.4 243,922 + Pf1N1B4_5496 0.17 +1.6 243,923 - Pf1N1B4_5496 0.18 -2.3 243,923 - Pf1N1B4_5496 0.18 -0.2 243,923 - Pf1N1B4_5496 0.18 +0.8 244,000 + Pf1N1B4_5496 0.52 +1.0 244,001 - Pf1N1B4_5496 0.52 -0.2 244,106 - -1.9 244,129 - +1.1 244,129 - -1.2 244,154 + -0.2 244,155 - +0.5 244,155 - -3.0 244,155 - -2.0 244,183 - +0.6 244,183 - +1.4 244,183 - +1.1 244,183 - +0.8 244,183 - +0.4 244,183 - -1.9 244,300 + -0.8 244,431 - -2.0 244,431 - -0.4 244,445 - +0.5 244,571 - -0.1 244,571 - +0.5 244,571 - -0.8 244,571 - -0.4
Or see this region's nucleotide sequence