Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5476 and Pf1N1B4_5477 are separated by 90 nucleotides
Pf1N1B4_5476 - hypothetical protein, at 227,952 to 228,143
_5476
Pf1N1B4_5477 - hypothetical protein, at 228,234 to 229,283
_5477
Position (kb) 227
228
229 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 227.017 kb on + strand at 227.017 kb on + strand at 227.017 kb on + strand at 227.017 kb on + strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.018 kb on - strand at 227.072 kb on + strand at 227.072 kb on + strand at 227.193 kb on + strand at 227.281 kb on + strand at 227.281 kb on + strand at 227.282 kb on - strand at 227.282 kb on - strand at 227.336 kb on + strand at 227.336 kb on + strand at 227.336 kb on + strand at 227.336 kb on + strand at 227.336 kb on + strand at 227.337 kb on - strand at 227.343 kb on + strand at 227.344 kb on - strand at 227.344 kb on - strand at 227.422 kb on + strand at 227.422 kb on + strand at 227.422 kb on + strand at 227.423 kb on - strand at 227.423 kb on - strand at 227.423 kb on - strand at 227.433 kb on + strand at 227.433 kb on + strand at 227.433 kb on + strand at 227.434 kb on - strand at 227.434 kb on - strand at 227.491 kb on + strand at 227.491 kb on + strand at 227.491 kb on + strand at 227.509 kb on + strand at 227.509 kb on + strand at 227.550 kb on + strand at 227.550 kb on + strand at 227.550 kb on + strand at 227.550 kb on + strand at 227.626 kb on + strand at 227.626 kb on + strand at 227.626 kb on + strand at 227.626 kb on + strand at 227.627 kb on - strand at 227.627 kb on - strand at 227.627 kb on - strand at 227.627 kb on - strand at 227.631 kb on - strand at 227.659 kb on - strand at 227.694 kb on + strand at 227.695 kb on - strand at 227.776 kb on + strand at 227.776 kb on + strand at 227.777 kb on - strand at 227.777 kb on - strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.778 kb on + strand at 227.779 kb on - strand at 227.779 kb on - strand at 227.779 kb on - strand at 227.779 kb on - strand at 227.779 kb on - strand at 227.779 kb on - strand at 227.779 kb on - strand at 228.007 kb on + strand, within Pf1N1B4_5476 at 228.007 kb on + strand at 228.007 kb on + strand, within Pf1N1B4_5476 at 228.007 kb on + strand, within Pf1N1B4_5476 at 228.008 kb on - strand, within Pf1N1B4_5476 at 228.194 kb on + strand at 228.194 kb on + strand at 228.281 kb on + strand at 228.306 kb on + strand at 228.307 kb on - strand at 228.307 kb on - strand at 228.330 kb on + strand at 228.373 kb on + strand, within Pf1N1B4_5477 at 228.374 kb on - strand, within Pf1N1B4_5477 at 228.374 kb on - strand, within Pf1N1B4_5477 at 228.374 kb on - strand, within Pf1N1B4_5477 at 228.446 kb on - strand, within Pf1N1B4_5477 at 228.479 kb on + strand, within Pf1N1B4_5477 at 228.479 kb on + strand, within Pf1N1B4_5477 at 228.479 kb on + strand, within Pf1N1B4_5477 at 228.479 kb on + strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.480 kb on - strand, within Pf1N1B4_5477 at 228.486 kb on + strand, within Pf1N1B4_5477 at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.487 kb on - strand at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.487 kb on - strand, within Pf1N1B4_5477 at 228.498 kb on + strand, within Pf1N1B4_5477 at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.499 kb on - strand at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.499 kb on - strand at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.499 kb on - strand, within Pf1N1B4_5477 at 228.585 kb on - strand, within Pf1N1B4_5477 at 228.729 kb on - strand, within Pf1N1B4_5477 at 228.729 kb on - strand, within Pf1N1B4_5477 at 228.752 kb on + strand, within Pf1N1B4_5477 at 228.753 kb on - strand at 228.753 kb on - strand, within Pf1N1B4_5477 at 228.781 kb on + strand, within Pf1N1B4_5477 at 228.781 kb on + strand at 228.782 kb on - strand, within Pf1N1B4_5477 at 228.782 kb on - strand, within Pf1N1B4_5477 at 228.782 kb on - strand, within Pf1N1B4_5477 at 228.794 kb on - strand, within Pf1N1B4_5477 at 228.865 kb on - strand, within Pf1N1B4_5477 at 228.865 kb on - strand, within Pf1N1B4_5477 at 228.934 kb on + strand, within Pf1N1B4_5477 at 228.934 kb on + strand, within Pf1N1B4_5477 at 228.934 kb on + strand, within Pf1N1B4_5477 at 228.934 kb on + strand at 228.935 kb on - strand, within Pf1N1B4_5477 at 228.935 kb on - strand, within Pf1N1B4_5477 at 228.935 kb on - strand, within Pf1N1B4_5477 at 228.935 kb on - strand, within Pf1N1B4_5477 at 229.017 kb on - strand, within Pf1N1B4_5477 at 229.017 kb on - strand, within Pf1N1B4_5477 at 229.084 kb on + strand, within Pf1N1B4_5477 at 229.085 kb on - strand, within Pf1N1B4_5477 at 229.085 kb on - strand at 229.085 kb on - strand, within Pf1N1B4_5477 at 229.085 kb on - strand, within Pf1N1B4_5477 at 229.085 kb on - strand, within Pf1N1B4_5477 at 229.085 kb on - strand, within Pf1N1B4_5477
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 227,017 + -0.8 227,017 + +0.8 227,017 + -0.2 227,017 + +1.2 227,018 - +0.0 227,018 - -0.5 227,018 - -0.6 227,018 - -1.4 227,018 - -0.2 227,018 - -1.2 227,018 - -0.8 227,018 - -1.0 227,018 - -2.0 227,018 - -2.2 227,072 + -1.5 227,072 + +0.4 227,193 + +0.8 227,281 + -2.0 227,281 + -0.2 227,282 - +0.2 227,282 - +0.0 227,336 + -2.0 227,336 + -1.1 227,336 + +1.1 227,336 + -0.2 227,336 + +0.1 227,337 - +0.2 227,343 + -0.2 227,344 - -0.8 227,344 - -1.0 227,422 + +0.8 227,422 + -0.8 227,422 + +1.6 227,423 - -0.2 227,423 - +1.4 227,423 - -0.4 227,433 + -2.0 227,433 + -0.8 227,433 + +0.1 227,434 - -0.9 227,434 - -0.8 227,491 + -0.5 227,491 + +0.3 227,491 + +0.0 227,509 + -4.7 227,509 + -2.4 227,550 + +0.2 227,550 + +0.8 227,550 + +0.6 227,550 + -0.1 227,626 + -0.5 227,626 + -0.7 227,626 + +2.2 227,626 + -1.3 227,627 - -2.2 227,627 - +1.1 227,627 - -2.7 227,627 - -1.5 227,631 - -1.5 227,659 - +0.8 227,694 + +0.4 227,695 - -1.8 227,776 + -0.4 227,776 + +0.5 227,777 - -0.4 227,777 - -0.2 227,778 + -0.5 227,778 + -0.5 227,778 + +0.8 227,778 + -1.2 227,778 + +1.0 227,778 + -0.8 227,778 + -2.5 227,778 + +1.0 227,778 + -0.1 227,778 + -0.2 227,778 + -2.8 227,778 + -0.8 227,779 - +0.4 227,779 - +0.3 227,779 - +0.8 227,779 - -1.2 227,779 - +0.8 227,779 - -1.4 227,779 - -0.4 228,007 + Pf1N1B4_5476 0.29 +0.8 228,007 + -0.2 228,007 + Pf1N1B4_5476 0.29 -3.2 228,007 + Pf1N1B4_5476 0.29 -1.5 228,008 - Pf1N1B4_5476 0.29 -0.2 228,194 + +0.8 228,194 + -0.1 228,281 + +1.6 228,306 + -1.9 228,307 - -1.2 228,307 - -2.4 228,330 + +0.8 228,373 + Pf1N1B4_5477 0.13 -2.7 228,374 - Pf1N1B4_5477 0.13 +0.3 228,374 - Pf1N1B4_5477 0.13 +0.6 228,374 - Pf1N1B4_5477 0.13 +1.5 228,446 - Pf1N1B4_5477 0.20 -1.5 228,479 + Pf1N1B4_5477 0.23 -2.4 228,479 + Pf1N1B4_5477 0.23 -0.5 228,479 + Pf1N1B4_5477 0.23 -0.2 228,479 + Pf1N1B4_5477 0.23 +0.6 228,480 - Pf1N1B4_5477 0.23 -2.2 228,480 - Pf1N1B4_5477 0.23 -0.7 228,480 - Pf1N1B4_5477 0.23 -1.8 228,480 - Pf1N1B4_5477 0.23 -0.7 228,480 - Pf1N1B4_5477 0.23 -0.6 228,480 - Pf1N1B4_5477 0.23 -2.5 228,480 - Pf1N1B4_5477 0.23 +0.8 228,480 - Pf1N1B4_5477 0.23 +0.5 228,480 - Pf1N1B4_5477 0.23 +0.8 228,480 - Pf1N1B4_5477 0.23 -3.2 228,480 - Pf1N1B4_5477 0.23 -1.2 228,480 - Pf1N1B4_5477 0.23 +0.2 228,480 - Pf1N1B4_5477 0.23 -1.5 228,480 - Pf1N1B4_5477 0.23 -0.1 228,486 + Pf1N1B4_5477 0.24 -1.2 228,487 - Pf1N1B4_5477 0.24 +0.1 228,487 - Pf1N1B4_5477 0.24 -2.0 228,487 - +0.8 228,487 - Pf1N1B4_5477 0.24 +2.0 228,487 - Pf1N1B4_5477 0.24 -0.2 228,487 - Pf1N1B4_5477 0.24 +0.2 228,487 - Pf1N1B4_5477 0.24 -1.8 228,498 + Pf1N1B4_5477 0.25 -1.8 228,499 - Pf1N1B4_5477 0.25 -0.6 228,499 - +2.6 228,499 - Pf1N1B4_5477 0.25 -0.4 228,499 - Pf1N1B4_5477 0.25 -1.5 228,499 - Pf1N1B4_5477 0.25 +0.4 228,499 - -1.2 228,499 - Pf1N1B4_5477 0.25 +1.4 228,499 - Pf1N1B4_5477 0.25 +0.6 228,585 - Pf1N1B4_5477 0.33 -0.0 228,729 - Pf1N1B4_5477 0.47 -0.2 228,729 - Pf1N1B4_5477 0.47 +0.2 228,752 + Pf1N1B4_5477 0.49 -0.8 228,753 - +1.4 228,753 - Pf1N1B4_5477 0.49 +1.4 228,781 + Pf1N1B4_5477 0.52 -1.5 228,781 + +2.9 228,782 - Pf1N1B4_5477 0.52 -0.2 228,782 - Pf1N1B4_5477 0.52 -2.7 228,782 - Pf1N1B4_5477 0.52 +3.4 228,794 - Pf1N1B4_5477 0.53 -0.2 228,865 - Pf1N1B4_5477 0.60 +0.4 228,865 - Pf1N1B4_5477 0.60 -1.8 228,934 + Pf1N1B4_5477 0.67 -1.0 228,934 + Pf1N1B4_5477 0.67 -1.0 228,934 + Pf1N1B4_5477 0.67 -1.2 228,934 + -2.0 228,935 - Pf1N1B4_5477 0.67 +0.8 228,935 - Pf1N1B4_5477 0.67 +1.2 228,935 - Pf1N1B4_5477 0.67 -2.5 228,935 - Pf1N1B4_5477 0.67 -1.8 229,017 - Pf1N1B4_5477 0.75 -1.0 229,017 - Pf1N1B4_5477 0.75 +2.1 229,084 + Pf1N1B4_5477 0.81 -1.8 229,085 - Pf1N1B4_5477 0.81 -1.4 229,085 - -1.2 229,085 - Pf1N1B4_5477 0.81 -0.5 229,085 - Pf1N1B4_5477 0.81 +1.3 229,085 - Pf1N1B4_5477 0.81 -2.8 229,085 - Pf1N1B4_5477 0.81 -0.1
Or see this region's nucleotide sequence