Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_545 and Pf1N1B4_546 are separated by 387 nucleotides Pf1N1B4_546 and Pf1N1B4_547 are separated by 229 nucleotides Pf1N1B4_547 and Pf1N1B4_548 are separated by 49 nucleotides
Pf1N1B4_545 - MadN protein, at 642,894 to 643,760
_545
Pf1N1B4_546 - FMN-dependent NADH-azoreductase, at 644,148 to 644,747
_546
Pf1N1B4_547 - 3-phosphoglycerate kinase, at 644,977 to 645,306
_547
Pf1N1B4_548 - Transcriptional regulator, LysR family, at 645,356 to 646,279
_548
Position (kb) 644
645 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.235 kb on - strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.292 kb on - strand, within Pf1N1B4_545 at 643.534 kb on + strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand at 643.675 kb on + strand at 643.676 kb on - strand at 643.676 kb on - strand at 643.700 kb on - strand at 643.700 kb on - strand at 643.749 kb on + strand at 643.750 kb on - strand at 643.824 kb on + strand at 643.824 kb on + strand at 643.824 kb on + strand at 643.824 kb on + strand at 643.824 kb on + strand at 643.825 kb on - strand at 643.825 kb on - strand at 643.828 kb on - strand at 643.828 kb on - strand at 643.912 kb on + strand at 643.919 kb on + strand at 643.919 kb on + strand at 643.919 kb on + strand at 643.976 kb on + strand at 643.977 kb on - strand at 643.977 kb on - strand at 643.977 kb on - strand at 644.115 kb on - strand at 644.115 kb on - strand at 644.130 kb on + strand at 644.131 kb on - strand at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.314 kb on + strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.458 kb on + strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.476 kb on - strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.482 kb on - strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.616 kb on - strand at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.755 kb on - strand at 644.755 kb on - strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.828 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.973 kb on - strand at 644.973 kb on - strand at 645.003 kb on + strand at 645.004 kb on - strand at 645.140 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand at 645.177 kb on + strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.191 kb on + strand, within Pf1N1B4_547 at 645.192 kb on - strand, within Pf1N1B4_547 at 645.237 kb on - strand, within Pf1N1B4_547 at 645.291 kb on + strand at 645.291 kb on + strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.470 kb on + strand, within Pf1N1B4_548 at 645.470 kb on + strand, within Pf1N1B4_548 at 645.471 kb on - strand at 645.471 kb on - strand, within Pf1N1B4_548 at 645.513 kb on + strand, within Pf1N1B4_548
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 643,228 + Pf1N1B4_545 0.39 +0.7 643,228 + Pf1N1B4_545 0.39 +1.1 643,228 + Pf1N1B4_545 0.39 -1.7 643,229 - Pf1N1B4_545 0.39 -0.2 643,229 - Pf1N1B4_545 0.39 -0.3 643,229 - Pf1N1B4_545 0.39 -0.8 643,229 - Pf1N1B4_545 0.39 -2.0 643,229 - Pf1N1B4_545 0.39 -1.5 643,235 - Pf1N1B4_545 0.39 +0.5 643,291 + Pf1N1B4_545 0.46 -0.0 643,291 + Pf1N1B4_545 0.46 -1.2 643,292 - Pf1N1B4_545 0.46 -0.4 643,534 + Pf1N1B4_545 0.74 +1.8 643,547 - Pf1N1B4_545 0.75 -1.3 643,547 - Pf1N1B4_545 0.75 -2.5 643,547 - Pf1N1B4_545 0.75 +1.5 643,547 - +1.1 643,675 + +1.7 643,676 - -2.0 643,676 - +1.5 643,700 - +0.6 643,700 - +0.6 643,749 + +1.1 643,750 - -0.5 643,824 + +0.6 643,824 + -1.2 643,824 + +1.3 643,824 + +0.9 643,824 + +0.3 643,825 - -0.9 643,825 - -0.9 643,828 - +0.9 643,828 - -0.0 643,912 + +1.3 643,919 + +1.4 643,919 + +1.6 643,919 + -0.1 643,976 + +1.4 643,977 - -0.5 643,977 - -0.2 643,977 - -0.9 644,115 - -1.2 644,115 - -0.3 644,130 + +1.1 644,131 - -0.5 644,281 + Pf1N1B4_546 0.22 +0.7 644,281 + Pf1N1B4_546 0.22 -0.5 644,281 + Pf1N1B4_546 0.22 +1.6 644,281 + Pf1N1B4_546 0.22 +1.4 644,282 - Pf1N1B4_546 0.22 -0.3 644,282 - Pf1N1B4_546 0.22 -0.9 644,282 - Pf1N1B4_546 0.22 +0.9 644,282 - Pf1N1B4_546 0.22 -0.1 644,314 + Pf1N1B4_546 0.28 +2.9 644,315 - Pf1N1B4_546 0.28 +0.6 644,315 - Pf1N1B4_546 0.28 -0.1 644,341 + Pf1N1B4_546 0.32 +1.3 644,341 + Pf1N1B4_546 0.32 +2.3 644,342 - Pf1N1B4_546 0.32 +0.4 644,342 - Pf1N1B4_546 0.32 +1.1 644,342 - Pf1N1B4_546 0.32 -0.2 644,386 + Pf1N1B4_546 0.40 +1.1 644,386 + Pf1N1B4_546 0.40 +1.3 644,387 - Pf1N1B4_546 0.40 +2.0 644,387 - Pf1N1B4_546 0.40 +1.1 644,458 + Pf1N1B4_546 0.52 +1.5 644,459 - Pf1N1B4_546 0.52 -0.0 644,459 - Pf1N1B4_546 0.52 +1.7 644,476 - Pf1N1B4_546 0.55 -2.9 644,481 + Pf1N1B4_546 0.56 +2.3 644,481 + Pf1N1B4_546 0.56 +1.6 644,481 + Pf1N1B4_546 0.56 +0.7 644,482 - Pf1N1B4_546 0.56 +0.3 644,615 + Pf1N1B4_546 0.78 +0.8 644,615 + Pf1N1B4_546 0.78 +2.0 644,615 + Pf1N1B4_546 0.78 +2.1 644,615 + Pf1N1B4_546 0.78 +2.5 644,615 + Pf1N1B4_546 0.78 -1.9 644,615 + Pf1N1B4_546 0.78 +1.6 644,615 + Pf1N1B4_546 0.78 +2.8 644,615 + Pf1N1B4_546 0.78 +1.5 644,616 - +3.1 644,616 - Pf1N1B4_546 0.78 +1.1 644,616 - Pf1N1B4_546 0.78 +0.0 644,616 - Pf1N1B4_546 0.78 +0.1 644,616 - Pf1N1B4_546 0.78 +0.1 644,616 - Pf1N1B4_546 0.78 +0.1 644,754 + -1.8 644,754 + -1.2 644,754 + +1.7 644,754 + +2.1 644,754 + +0.3 644,754 + +2.9 644,754 + +2.1 644,754 + +2.2 644,754 + +2.0 644,755 - +2.3 644,755 - -1.7 644,776 + +2.0 644,776 + +3.0 644,776 + +0.9 644,776 + +3.0 644,776 + +2.5 644,776 + +1.0 644,777 - -0.7 644,777 - +1.1 644,777 - -0.5 644,828 + +2.0 644,972 + -0.9 644,972 + +1.4 644,972 + +1.3 644,972 + -1.2 644,972 + +2.0 644,972 + +3.1 644,972 + +2.1 644,973 - -2.6 644,973 - +0.9 645,003 + +1.7 645,004 - -0.3 645,140 + Pf1N1B4_547 0.49 +0.6 645,177 + Pf1N1B4_547 0.61 +2.4 645,177 + +1.1 645,177 + Pf1N1B4_547 0.61 +0.8 645,178 - Pf1N1B4_547 0.61 +0.1 645,178 - Pf1N1B4_547 0.61 +0.8 645,191 + Pf1N1B4_547 0.65 +2.4 645,192 - Pf1N1B4_547 0.65 +0.6 645,237 - Pf1N1B4_547 0.79 +1.1 645,291 + -1.6 645,291 + +2.0 645,292 - -0.2 645,292 - -1.5 645,292 - +0.1 645,292 - -0.8 645,337 - -1.2 645,337 - -0.5 645,337 - +0.0 645,397 - -1.7 645,397 - +0.4 645,397 - -0.5 645,397 - -0.0 645,470 + Pf1N1B4_548 0.12 +2.5 645,470 + Pf1N1B4_548 0.12 -0.5 645,471 - +1.7 645,471 - Pf1N1B4_548 0.12 -0.6 645,513 + Pf1N1B4_548 0.17 -0.9
Or see this region's nucleotide sequence