Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides Pf1N1B4_5430 and Pf1N1B4_5431 are separated by 720 nucleotides Pf1N1B4_5431 and Pf1N1B4_5432 are separated by 94 nucleotides
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Pf1N1B4_5431 - hypothetical protein, at 170,517 to 171,065
_5431
Pf1N1B4_5432 - hypothetical protein, at 171,160 to 171,348
_5432
Position (kb) 170
171
172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand at 169.681 kb on + strand, within Pf1N1B4_5430 at 169.682 kb on - strand, within Pf1N1B4_5430 at 169.747 kb on - strand at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.751 kb on - strand at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.809 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.899 kb on + strand at 169.899 kb on + strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.911 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.017 kb on - strand at 170.069 kb on + strand at 170.082 kb on + strand at 170.083 kb on - strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.190 kb on - strand at 170.190 kb on - strand at 170.244 kb on + strand at 170.244 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.282 kb on + strand at 170.368 kb on + strand at 170.368 kb on + strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.553 kb on + strand at 170.554 kb on - strand at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.739 kb on - strand, within Pf1N1B4_5431 at 170.746 kb on + strand, within Pf1N1B4_5431 at 170.746 kb on + strand, within Pf1N1B4_5431 at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.747 kb on - strand at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.899 kb on + strand, within Pf1N1B4_5431 at 170.899 kb on + strand at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.956 kb on + strand, within Pf1N1B4_5431 at 171.076 kb on + strand at 171.100 kb on - strand at 171.109 kb on + strand at 171.109 kb on + strand at 171.109 kb on + strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.158 kb on - strand at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.235 kb on + strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.365 kb on - strand at 171.416 kb on + strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.561 kb on - strand at 171.585 kb on - strand at 171.667 kb on - strand at 171.705 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.734 kb on - strand at 171.756 kb on - strand at 171.761 kb on + strand at 171.762 kb on - strand at 171.762 kb on - strand at 171.849 kb on - strand at 171.855 kb on + strand at 171.887 kb on - strand at 171.892 kb on + strand at 171.954 kb on + strand at 171.954 kb on + strand at 171.965 kb on + strand at 171.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 169,549 + -0.5 169,549 + +0.2 169,549 + +0.0 169,549 + +0.1 169,549 + -1.5 169,584 + +0.5 169,584 + +0.0 169,584 + +0.1 169,585 - -0.8 169,585 - -0.2 169,631 - -0.4 169,681 + Pf1N1B4_5430 0.31 +0.4 169,682 - Pf1N1B4_5430 0.32 -1.5 169,747 - -0.2 169,750 + Pf1N1B4_5430 0.72 +0.1 169,750 + Pf1N1B4_5430 0.72 -1.1 169,751 - -0.2 169,751 - Pf1N1B4_5430 0.73 -0.2 169,751 - Pf1N1B4_5430 0.73 -2.2 169,751 - Pf1N1B4_5430 0.73 -0.8 169,751 - Pf1N1B4_5430 0.73 -0.8 169,792 + +1.2 169,792 + -2.9 169,792 + +0.8 169,792 + -0.1 169,793 - -0.2 169,793 - -0.5 169,793 - +1.0 169,793 - -0.6 169,793 - -1.8 169,809 + -0.1 169,895 + +0.4 169,895 + +0.7 169,895 + +0.5 169,895 + -1.0 169,895 + -1.0 169,896 - +0.8 169,896 - +1.8 169,896 - +0.5 169,896 - +2.1 169,896 - +1.1 169,896 - -0.8 169,896 - +2.2 169,896 - +0.8 169,899 + -0.1 169,899 + +2.4 169,900 - -0.8 169,900 - -0.2 169,900 - +2.5 169,911 + +1.7 170,016 + +1.2 170,016 + -0.8 170,016 + +0.4 170,017 - -1.2 170,069 + +1.0 170,082 + +0.6 170,083 - +0.2 170,156 + +0.2 170,156 + -0.8 170,156 + +0.6 170,157 - +0.0 170,157 - +0.2 170,157 - +1.8 170,157 - -3.5 170,190 - +0.4 170,190 - -1.2 170,244 + -0.4 170,244 + +0.2 170,256 + -2.0 170,256 + -1.3 170,256 + -1.2 170,282 + -2.0 170,368 + -0.8 170,368 + +1.9 170,369 - +1.1 170,369 - +0.4 170,369 - -1.2 170,369 - -1.2 170,369 - -0.2 170,553 + +1.1 170,554 - -2.4 170,620 + Pf1N1B4_5431 0.19 +1.7 170,620 + Pf1N1B4_5431 0.19 -2.9 170,620 + Pf1N1B4_5431 0.19 -0.6 170,620 + Pf1N1B4_5431 0.19 -0.1 170,620 + Pf1N1B4_5431 0.19 +0.0 170,620 + Pf1N1B4_5431 0.19 +0.8 170,620 + Pf1N1B4_5431 0.19 -2.0 170,620 + Pf1N1B4_5431 0.19 +2.7 170,620 + Pf1N1B4_5431 0.19 -0.3 170,620 + Pf1N1B4_5431 0.19 +0.8 170,621 - Pf1N1B4_5431 0.19 +0.4 170,621 - Pf1N1B4_5431 0.19 -0.5 170,621 - Pf1N1B4_5431 0.19 +2.3 170,621 - Pf1N1B4_5431 0.19 -2.5 170,621 - Pf1N1B4_5431 0.19 +1.6 170,621 - Pf1N1B4_5431 0.19 +1.2 170,621 - +0.4 170,621 - Pf1N1B4_5431 0.19 -1.5 170,621 - +0.8 170,621 - Pf1N1B4_5431 0.19 +3.9 170,739 - Pf1N1B4_5431 0.40 -1.8 170,746 + Pf1N1B4_5431 0.42 +0.5 170,746 + Pf1N1B4_5431 0.42 -0.8 170,747 - Pf1N1B4_5431 0.42 -0.5 170,747 - -0.5 170,747 - Pf1N1B4_5431 0.42 +0.4 170,747 - Pf1N1B4_5431 0.42 -1.2 170,899 + Pf1N1B4_5431 0.70 +2.0 170,899 + +1.1 170,900 - Pf1N1B4_5431 0.70 -0.8 170,900 - Pf1N1B4_5431 0.70 -1.5 170,900 - Pf1N1B4_5431 0.70 -1.5 170,956 + Pf1N1B4_5431 0.80 -2.2 171,076 + -0.6 171,100 - -0.3 171,109 + +0.5 171,109 + +2.5 171,109 + +1.8 171,110 - -0.8 171,110 - -1.2 171,110 - -0.9 171,110 - -0.2 171,158 - -0.4 171,229 + Pf1N1B4_5432 0.37 -0.2 171,229 + Pf1N1B4_5432 0.37 -1.0 171,229 + Pf1N1B4_5432 0.37 -0.7 171,229 + Pf1N1B4_5432 0.37 -1.8 171,230 - Pf1N1B4_5432 0.37 -0.2 171,230 - Pf1N1B4_5432 0.37 -0.8 171,230 - Pf1N1B4_5432 0.37 +2.6 171,230 - Pf1N1B4_5432 0.37 -1.2 171,230 - Pf1N1B4_5432 0.37 -2.2 171,230 - Pf1N1B4_5432 0.37 +0.4 171,230 - Pf1N1B4_5432 0.37 +0.8 171,235 + Pf1N1B4_5432 0.40 +0.2 171,291 - Pf1N1B4_5432 0.69 +2.1 171,291 - Pf1N1B4_5432 0.69 -0.3 171,365 - -0.2 171,416 + +0.9 171,417 - +0.4 171,417 - -2.0 171,417 - -1.5 171,417 - -0.8 171,417 - +1.8 171,561 - +1.4 171,585 - -0.3 171,667 - -0.0 171,705 + -0.2 171,729 + +1.1 171,729 + +0.2 171,729 + +0.0 171,729 + -1.5 171,730 - +2.5 171,730 - -1.4 171,730 - -2.0 171,730 - -1.0 171,733 + -1.0 171,733 + -2.7 171,733 + -2.2 171,734 - +0.9 171,756 - -0.8 171,761 + -1.2 171,762 - -1.8 171,762 - -0.5 171,849 - +1.0 171,855 + -1.0 171,887 - +0.0 171,892 + +1.2 171,954 + +0.1 171,954 + -1.2 171,965 + -1.8 171,966 - +0.3
Or see this region's nucleotide sequence