Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5428 and Pf1N1B4_5429 are separated by 253 nucleotides Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides Pf1N1B4_5430 and Pf1N1B4_5431 are separated by 720 nucleotides
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Pf1N1B4_5431 - hypothetical protein, at 170,517 to 171,065
_5431
Position (kb) 168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428 at 168.196 kb on + strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.201 kb on - strand, within Pf1N1B4_5428 at 168.381 kb on + strand, within Pf1N1B4_5428 at 168.391 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.420 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.580 kb on + strand at 168.581 kb on - strand at 168.660 kb on - strand at 168.728 kb on + strand at 168.729 kb on - strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.732 kb on - strand at 168.747 kb on + strand at 168.747 kb on + strand at 168.906 kb on - strand at 168.977 kb on + strand, within Pf1N1B4_5429 at 168.978 kb on - strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.176 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.329 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.338 kb on - strand, within Pf1N1B4_5429 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand at 169.681 kb on + strand, within Pf1N1B4_5430 at 169.682 kb on - strand, within Pf1N1B4_5430 at 169.747 kb on - strand at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.751 kb on - strand at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.809 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.899 kb on + strand at 169.899 kb on + strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.911 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.017 kb on - strand at 170.069 kb on + strand at 170.082 kb on + strand at 170.083 kb on - strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.190 kb on - strand at 170.190 kb on - strand at 170.244 kb on + strand at 170.244 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.282 kb on + strand at 170.368 kb on + strand at 170.368 kb on + strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 168,034 + Pf1N1B4_5428 0.38 -2.8 168,035 - Pf1N1B4_5428 0.38 +0.8 168,052 + Pf1N1B4_5428 0.40 -1.1 168,053 - Pf1N1B4_5428 0.40 +0.0 168,053 - +0.8 168,069 + Pf1N1B4_5428 0.42 -0.8 168,070 - Pf1N1B4_5428 0.42 +0.5 168,130 + Pf1N1B4_5428 0.48 +0.9 168,131 - Pf1N1B4_5428 0.48 -0.2 168,131 - Pf1N1B4_5428 0.48 -2.4 168,131 - Pf1N1B4_5428 0.48 +0.8 168,131 - Pf1N1B4_5428 0.48 +1.2 168,133 + Pf1N1B4_5428 0.48 -2.2 168,133 + Pf1N1B4_5428 0.48 -1.3 168,133 + Pf1N1B4_5428 0.48 +0.6 168,133 + Pf1N1B4_5428 0.48 -1.4 168,133 + Pf1N1B4_5428 0.48 -0.5 168,133 + Pf1N1B4_5428 0.48 -2.5 168,134 - Pf1N1B4_5428 0.48 -1.2 168,134 - Pf1N1B4_5428 0.48 -1.5 168,145 + Pf1N1B4_5428 0.49 +0.8 168,196 + Pf1N1B4_5428 0.54 -1.8 168,197 - Pf1N1B4_5428 0.55 -0.7 168,197 - Pf1N1B4_5428 0.55 +0.0 168,197 - Pf1N1B4_5428 0.55 -2.1 168,197 - Pf1N1B4_5428 0.55 +1.0 168,201 - Pf1N1B4_5428 0.55 -2.3 168,381 + Pf1N1B4_5428 0.73 +0.0 168,391 + Pf1N1B4_5428 0.74 +0.5 168,415 + Pf1N1B4_5428 0.77 -1.1 168,415 + Pf1N1B4_5428 0.77 +0.8 168,416 - Pf1N1B4_5428 0.77 -2.5 168,416 - Pf1N1B4_5428 0.77 -1.5 168,419 + Pf1N1B4_5428 0.77 -0.2 168,419 + Pf1N1B4_5428 0.77 -2.4 168,419 + Pf1N1B4_5428 0.77 -0.4 168,419 + Pf1N1B4_5428 0.77 -1.4 168,419 + Pf1N1B4_5428 0.77 +0.6 168,420 - Pf1N1B4_5428 0.77 -0.1 168,524 - Pf1N1B4_5428 0.88 +2.0 168,524 - Pf1N1B4_5428 0.88 +1.9 168,580 + +0.5 168,581 - -1.5 168,660 - -0.6 168,728 + +0.2 168,729 - +1.1 168,731 + -1.1 168,731 + -0.2 168,731 + +0.5 168,732 - +1.4 168,747 + +0.6 168,747 + -0.8 168,906 - -2.2 168,977 + Pf1N1B4_5429 0.12 +1.5 168,978 - Pf1N1B4_5429 0.12 +0.5 169,103 + Pf1N1B4_5429 0.32 -1.1 169,103 + Pf1N1B4_5429 0.32 +1.9 169,175 + Pf1N1B4_5429 0.43 +1.3 169,175 + Pf1N1B4_5429 0.43 +1.6 169,175 + Pf1N1B4_5429 0.43 +0.2 169,175 + Pf1N1B4_5429 0.43 -1.4 169,175 + Pf1N1B4_5429 0.43 -0.2 169,176 - Pf1N1B4_5429 0.43 -0.2 169,304 - Pf1N1B4_5429 0.63 -1.5 169,304 - Pf1N1B4_5429 0.63 -1.5 169,329 + Pf1N1B4_5429 0.67 -1.5 169,337 + Pf1N1B4_5429 0.68 +1.3 169,337 + Pf1N1B4_5429 0.68 -2.5 169,337 + Pf1N1B4_5429 0.68 -0.8 169,338 - Pf1N1B4_5429 0.68 +0.4 169,549 + -0.5 169,549 + +0.2 169,549 + +0.0 169,549 + +0.1 169,549 + -1.5 169,584 + +0.5 169,584 + +0.0 169,584 + +0.1 169,585 - -0.8 169,585 - -0.2 169,631 - -0.4 169,681 + Pf1N1B4_5430 0.31 +0.4 169,682 - Pf1N1B4_5430 0.32 -1.5 169,747 - -0.2 169,750 + Pf1N1B4_5430 0.72 +0.1 169,750 + Pf1N1B4_5430 0.72 -1.1 169,751 - -0.2 169,751 - Pf1N1B4_5430 0.73 -0.2 169,751 - Pf1N1B4_5430 0.73 -2.2 169,751 - Pf1N1B4_5430 0.73 -0.8 169,751 - Pf1N1B4_5430 0.73 -0.8 169,792 + +1.2 169,792 + -2.9 169,792 + +0.8 169,792 + -0.1 169,793 - -0.2 169,793 - -0.5 169,793 - +1.0 169,793 - -0.6 169,793 - -1.8 169,809 + -0.1 169,895 + +0.4 169,895 + +0.7 169,895 + +0.5 169,895 + -1.0 169,895 + -1.0 169,896 - +0.8 169,896 - +1.8 169,896 - +0.5 169,896 - +2.1 169,896 - +1.1 169,896 - -0.8 169,896 - +2.2 169,896 - +0.8 169,899 + -0.1 169,899 + +2.4 169,900 - -0.8 169,900 - -0.2 169,900 - +2.5 169,911 + +1.7 170,016 + +1.2 170,016 + -0.8 170,016 + +0.4 170,017 - -1.2 170,069 + +1.0 170,082 + +0.6 170,083 - +0.2 170,156 + +0.2 170,156 + -0.8 170,156 + +0.6 170,157 - +0.0 170,157 - +0.2 170,157 - +1.8 170,157 - -3.5 170,190 - +0.4 170,190 - -1.2 170,244 + -0.4 170,244 + +0.2 170,256 + -2.0 170,256 + -1.3 170,256 + -1.2 170,282 + -2.0 170,368 + -0.8 170,368 + +1.9 170,369 - +1.1 170,369 - +0.4 170,369 - -1.2 170,369 - -1.2 170,369 - -0.2
Or see this region's nucleotide sequence