Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5425 and Pf1N1B4_5426 are separated by 461 nucleotides Pf1N1B4_5426 and Pf1N1B4_5427 are separated by 130 nucleotides Pf1N1B4_5427 and Pf1N1B4_5428 are separated by 474 nucleotides
Pf1N1B4_5425 - DNA-binding protein HU-beta, at 165,496 to 165,771
_5425
Pf1N1B4_5426 - hypothetical protein, at 166,233 to 166,580
_5426
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Position (kb) 166
167
168 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 165.724 kb on + strand, within Pf1N1B4_5425 at 165.725 kb on - strand, within Pf1N1B4_5425 at 165.828 kb on - strand at 165.848 kb on + strand at 165.849 kb on - strand at 165.903 kb on - strand at 165.949 kb on + strand at 165.950 kb on - strand at 165.950 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.128 kb on - strand at 166.211 kb on - strand at 166.222 kb on + strand at 166.222 kb on + strand at 166.223 kb on - strand at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.347 kb on - strand, within Pf1N1B4_5426 at 166.371 kb on + strand, within Pf1N1B4_5426 at 166.372 kb on - strand, within Pf1N1B4_5426 at 166.439 kb on - strand, within Pf1N1B4_5426 at 166.532 kb on - strand, within Pf1N1B4_5426 at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.893 kb on - strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.977 kb on - strand at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 167.056 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.126 kb on - strand, within Pf1N1B4_5427 at 167.128 kb on - strand, within Pf1N1B4_5427 at 167.182 kb on + strand at 167.182 kb on + strand at 167.183 kb on - strand at 167.239 kb on - strand at 167.275 kb on - strand at 167.355 kb on - strand at 167.381 kb on - strand at 167.381 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.438 kb on - strand at 167.476 kb on - strand at 167.566 kb on - strand at 167.566 kb on - strand at 167.594 kb on + strand at 167.594 kb on + strand at 167.595 kb on - strand at 167.595 kb on - strand at 167.629 kb on + strand at 167.629 kb on + strand at 167.640 kb on - strand at 167.640 kb on - strand at 167.672 kb on - strand at 167.745 kb on + strand at 167.766 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.843 kb on - strand, within Pf1N1B4_5428 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 165,724 + Pf1N1B4_5425 0.83 +0.2 165,725 - Pf1N1B4_5425 0.83 +1.8 165,828 - +1.3 165,848 + +0.8 165,849 - -0.0 165,903 - -2.0 165,949 + -1.2 165,950 - +0.0 165,950 - -0.0 166,031 - -1.2 166,031 - -0.5 166,031 - +0.8 166,128 - +1.6 166,211 - -3.8 166,222 + -1.5 166,222 + +1.5 166,223 - +0.7 166,274 + Pf1N1B4_5426 0.12 -0.2 166,274 + Pf1N1B4_5426 0.12 -0.4 166,274 + Pf1N1B4_5426 0.12 -0.1 166,275 - Pf1N1B4_5426 0.12 -0.6 166,275 - Pf1N1B4_5426 0.12 -2.8 166,287 + Pf1N1B4_5426 0.16 -1.0 166,287 + Pf1N1B4_5426 0.16 +0.5 166,287 + Pf1N1B4_5426 0.16 +0.5 166,287 + Pf1N1B4_5426 0.16 -1.5 166,287 + Pf1N1B4_5426 0.16 +0.3 166,288 - Pf1N1B4_5426 0.16 +0.0 166,288 - Pf1N1B4_5426 0.16 -0.0 166,288 - Pf1N1B4_5426 0.16 +0.4 166,347 - Pf1N1B4_5426 0.33 +0.9 166,371 + Pf1N1B4_5426 0.40 -0.1 166,372 - Pf1N1B4_5426 0.40 +1.0 166,439 - Pf1N1B4_5426 0.59 +1.0 166,532 - Pf1N1B4_5426 0.86 -0.8 166,603 + -1.2 166,603 + -2.7 166,603 + +0.8 166,603 + -1.5 166,603 + -1.7 166,604 - +2.8 166,604 - -3.5 166,604 - -1.8 166,819 + Pf1N1B4_5427 0.23 +1.2 166,819 + Pf1N1B4_5427 0.23 +1.5 166,820 - Pf1N1B4_5427 0.23 -0.4 166,820 - Pf1N1B4_5427 0.23 -1.2 166,893 - Pf1N1B4_5427 0.38 -1.8 166,976 + Pf1N1B4_5427 0.56 -0.4 166,976 + Pf1N1B4_5427 0.56 +0.9 166,976 + Pf1N1B4_5427 0.56 -0.5 166,976 + Pf1N1B4_5427 0.56 -0.8 166,976 + Pf1N1B4_5427 0.56 +0.2 166,976 + Pf1N1B4_5427 0.56 +1.0 166,976 + Pf1N1B4_5427 0.56 -2.0 166,976 + Pf1N1B4_5427 0.56 +1.1 166,976 + Pf1N1B4_5427 0.56 -0.0 166,977 - -0.2 166,986 + Pf1N1B4_5427 0.58 -2.4 166,986 + Pf1N1B4_5427 0.58 -0.4 166,987 - Pf1N1B4_5427 0.58 +1.3 166,987 - Pf1N1B4_5427 0.58 +0.1 167,056 + Pf1N1B4_5427 0.73 +0.9 167,125 + Pf1N1B4_5427 0.87 -0.4 167,125 + Pf1N1B4_5427 0.87 -0.2 167,125 + Pf1N1B4_5427 0.87 -0.0 167,125 + Pf1N1B4_5427 0.87 -1.2 167,125 + Pf1N1B4_5427 0.87 +1.2 167,125 + Pf1N1B4_5427 0.87 -2.7 167,125 + Pf1N1B4_5427 0.87 +2.1 167,126 - Pf1N1B4_5427 0.88 +1.2 167,128 - Pf1N1B4_5427 0.88 +0.8 167,182 + +0.9 167,182 + +0.8 167,183 - -0.6 167,239 - -1.5 167,275 - -2.0 167,355 - -0.2 167,381 - +0.7 167,381 - +1.2 167,384 - -1.5 167,384 - -0.7 167,384 - +1.2 167,384 - +0.2 167,384 - +0.2 167,384 - +0.5 167,438 - +0.5 167,476 - +0.9 167,566 - -0.8 167,566 - +0.1 167,594 + -1.7 167,594 + -0.8 167,595 - +0.7 167,595 - -0.0 167,629 + +0.5 167,629 + +0.1 167,640 - +0.6 167,640 - -0.9 167,672 - -2.7 167,745 + +0.5 167,766 + Pf1N1B4_5428 0.11 -0.0 167,788 + Pf1N1B4_5428 0.13 -0.4 167,788 + Pf1N1B4_5428 0.13 +1.1 167,843 - Pf1N1B4_5428 0.19 -1.3 168,034 + Pf1N1B4_5428 0.38 -2.8 168,035 - Pf1N1B4_5428 0.38 +0.8 168,052 + Pf1N1B4_5428 0.40 -1.1 168,053 - Pf1N1B4_5428 0.40 +0.0 168,053 - +0.8 168,069 + Pf1N1B4_5428 0.42 -0.8 168,070 - Pf1N1B4_5428 0.42 +0.5 168,130 + Pf1N1B4_5428 0.48 +0.9 168,131 - Pf1N1B4_5428 0.48 -0.2 168,131 - Pf1N1B4_5428 0.48 -2.4 168,131 - Pf1N1B4_5428 0.48 +0.8 168,131 - Pf1N1B4_5428 0.48 +1.2 168,133 + Pf1N1B4_5428 0.48 -2.2 168,133 + Pf1N1B4_5428 0.48 -1.3 168,133 + Pf1N1B4_5428 0.48 +0.6 168,133 + Pf1N1B4_5428 0.48 -1.4 168,133 + Pf1N1B4_5428 0.48 -0.5 168,133 + Pf1N1B4_5428 0.48 -2.5 168,134 - Pf1N1B4_5428 0.48 -1.2 168,134 - Pf1N1B4_5428 0.48 -1.5 168,145 + Pf1N1B4_5428 0.49 +0.8
Or see this region's nucleotide sequence