Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5381 and Pf1N1B4_5382 are separated by 486 nucleotides Pf1N1B4_5382 and Pf1N1B4_5383 are separated by 853 nucleotides
Pf1N1B4_5381 - hypothetical protein, at 114,183 to 114,464
_5381
Pf1N1B4_5382 - hypothetical protein, at 114,951 to 115,394
_5382
Pf1N1B4_5383 - hypothetical protein, at 116,248 to 116,568
_5383
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.035 kb on + strand at 114.101 kb on + strand at 114.109 kb on - strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.164 kb on - strand at 114.181 kb on + strand at 114.181 kb on + strand at 114.281 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.320 kb on + strand, within Pf1N1B4_5381 at 114.321 kb on - strand, within Pf1N1B4_5381 at 114.343 kb on + strand at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.401 kb on - strand at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.405 kb on - strand, within Pf1N1B4_5381 at 114.405 kb on - strand, within Pf1N1B4_5381 at 114.434 kb on + strand, within Pf1N1B4_5381 at 114.434 kb on + strand, within Pf1N1B4_5381 at 114.532 kb on + strand at 114.620 kb on + strand at 114.620 kb on + strand at 114.637 kb on - strand at 114.684 kb on + strand at 114.797 kb on + strand at 114.797 kb on + strand at 114.994 kb on + strand at 114.995 kb on - strand at 114.995 kb on - strand at 115.027 kb on + strand, within Pf1N1B4_5382 at 115.028 kb on - strand, within Pf1N1B4_5382 at 115.210 kb on + strand, within Pf1N1B4_5382 at 115.211 kb on - strand, within Pf1N1B4_5382 at 115.211 kb on - strand, within Pf1N1B4_5382 at 115.298 kb on + strand, within Pf1N1B4_5382 at 115.299 kb on - strand, within Pf1N1B4_5382 at 115.299 kb on - strand, within Pf1N1B4_5382 at 115.406 kb on + strand at 115.406 kb on + strand at 115.415 kb on + strand at 115.415 kb on + strand at 115.415 kb on + strand at 115.416 kb on - strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.435 kb on - strand at 115.478 kb on + strand at 115.478 kb on + strand at 115.479 kb on - strand at 115.547 kb on + strand at 115.547 kb on + strand at 115.547 kb on + strand at 115.548 kb on - strand at 115.548 kb on - strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.645 kb on + strand at 115.646 kb on - strand at 115.646 kb on - strand at 115.646 kb on - strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.689 kb on + strand at 115.690 kb on - strand at 115.690 kb on - strand at 115.690 kb on - strand at 115.690 kb on - strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.723 kb on + strand at 115.724 kb on - strand at 115.736 kb on + strand at 115.736 kb on + strand at 115.882 kb on - strand at 115.902 kb on + strand at 115.902 kb on + strand at 115.902 kb on + strand at 115.948 kb on + strand at 115.948 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.952 kb on + strand at 115.953 kb on - strand at 115.976 kb on + strand at 115.976 kb on + strand at 115.976 kb on + strand at 115.976 kb on + strand at 115.989 kb on + strand at 115.989 kb on + strand at 116.000 kb on + strand at 116.000 kb on + strand at 116.000 kb on + strand at 116.001 kb on - strand at 116.036 kb on + strand at 116.036 kb on + strand at 116.036 kb on + strand at 116.036 kb on + strand at 116.037 kb on - strand at 116.037 kb on - strand at 116.093 kb on + strand at 116.093 kb on + strand at 116.093 kb on + strand at 116.093 kb on + strand at 116.093 kb on + strand at 116.093 kb on + strand at 116.094 kb on - strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.165 kb on - strand at 116.188 kb on + strand at 116.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 114,012 + +1.2 114,012 + -1.5 114,012 + -0.8 114,012 + -2.3 114,012 + -1.5 114,012 + -2.4 114,012 + -2.7 114,013 - -0.2 114,013 - +0.8 114,013 - -0.4 114,035 + -1.5 114,101 + -2.0 114,109 - +1.2 114,143 + -0.2 114,143 + +0.8 114,143 + -0.8 114,143 + -1.5 114,143 + -2.4 114,143 + +0.8 114,143 + +0.8 114,143 + -1.0 114,143 + -1.9 114,143 + -0.7 114,143 + -1.2 114,143 + -2.5 114,143 + -1.5 114,143 + +1.2 114,143 + -0.2 114,143 + -2.2 114,143 + -2.2 114,144 - -0.6 114,144 - +0.2 114,144 - +0.1 114,147 + -0.9 114,147 + -1.5 114,147 + -1.5 114,147 + +0.2 114,147 + -0.2 114,147 + -1.8 114,147 + -0.5 114,147 + -1.5 114,147 + -2.7 114,147 + -1.8 114,147 + +1.4 114,148 - +0.1 114,148 - +0.3 114,148 - +0.1 114,148 - -0.2 114,163 + -1.8 114,163 + -3.2 114,163 + -0.8 114,164 - +0.0 114,181 + +0.8 114,181 + -1.7 114,281 + Pf1N1B4_5381 0.35 -0.2 114,286 + Pf1N1B4_5381 0.37 -0.2 114,286 + Pf1N1B4_5381 0.37 +2.8 114,320 + Pf1N1B4_5381 0.49 -2.0 114,321 - Pf1N1B4_5381 0.49 -2.5 114,343 + -0.8 114,343 + Pf1N1B4_5381 0.57 -0.1 114,343 + Pf1N1B4_5381 0.57 -0.5 114,343 + Pf1N1B4_5381 0.57 -1.0 114,343 + Pf1N1B4_5381 0.57 +1.0 114,343 + Pf1N1B4_5381 0.57 -2.2 114,343 + Pf1N1B4_5381 0.57 +0.8 114,343 + Pf1N1B4_5381 0.57 -2.8 114,343 + Pf1N1B4_5381 0.57 -0.7 114,400 + Pf1N1B4_5381 0.77 -1.8 114,400 + Pf1N1B4_5381 0.77 +0.5 114,400 + Pf1N1B4_5381 0.77 -0.6 114,400 + Pf1N1B4_5381 0.77 -0.1 114,400 + Pf1N1B4_5381 0.77 -2.0 114,400 + Pf1N1B4_5381 0.77 -1.2 114,400 + Pf1N1B4_5381 0.77 -1.2 114,400 + Pf1N1B4_5381 0.77 -1.2 114,401 - -1.8 114,404 + Pf1N1B4_5381 0.78 -0.4 114,404 + Pf1N1B4_5381 0.78 +0.0 114,404 + Pf1N1B4_5381 0.78 -0.9 114,404 + Pf1N1B4_5381 0.78 -0.8 114,404 + Pf1N1B4_5381 0.78 -1.2 114,404 + Pf1N1B4_5381 0.78 -0.2 114,404 + Pf1N1B4_5381 0.78 -1.0 114,405 - Pf1N1B4_5381 0.79 +0.5 114,405 - Pf1N1B4_5381 0.79 -1.8 114,434 + Pf1N1B4_5381 0.89 -1.4 114,434 + Pf1N1B4_5381 0.89 -0.6 114,532 + -1.8 114,620 + -0.4 114,620 + +0.8 114,637 - +0.1 114,684 + +0.2 114,797 + -0.8 114,797 + -1.2 114,994 + +0.2 114,995 - +0.4 114,995 - +0.4 115,027 + Pf1N1B4_5382 0.17 -1.8 115,028 - Pf1N1B4_5382 0.17 +0.4 115,210 + Pf1N1B4_5382 0.58 -0.6 115,211 - Pf1N1B4_5382 0.59 -0.8 115,211 - Pf1N1B4_5382 0.59 -1.0 115,298 + Pf1N1B4_5382 0.78 -0.8 115,299 - Pf1N1B4_5382 0.78 +0.4 115,299 - Pf1N1B4_5382 0.78 +0.1 115,406 + -0.9 115,406 + -3.0 115,415 + -1.2 115,415 + +0.1 115,415 + -1.1 115,416 - -0.5 115,434 + -2.8 115,434 + -1.6 115,434 + -0.4 115,434 + -3.0 115,434 + -2.0 115,434 + +0.5 115,434 + -2.3 115,434 + -1.1 115,434 + -1.8 115,435 - +1.6 115,478 + +0.4 115,478 + -2.4 115,479 - -0.9 115,547 + -2.2 115,547 + -0.5 115,547 + -1.5 115,548 - -1.2 115,548 - -1.5 115,645 + -1.1 115,645 + -0.7 115,645 + -0.6 115,645 + -3.5 115,645 + -1.6 115,645 + -1.3 115,645 + -3.2 115,645 + -3.1 115,645 + -0.7 115,645 + -0.6 115,645 + -1.5 115,645 + -0.8 115,645 + -2.9 115,645 + -1.0 115,646 - -0.5 115,646 - -0.2 115,646 - -1.0 115,689 + -1.7 115,689 + -2.2 115,689 + -1.2 115,689 + -1.8 115,689 + -1.2 115,689 + -3.0 115,689 + -2.0 115,689 + -3.2 115,689 + -1.4 115,690 - -0.4 115,690 - -0.8 115,690 - -0.8 115,690 - -0.4 115,723 + -2.2 115,723 + -2.7 115,723 + -0.4 115,723 + -2.0 115,723 + -2.7 115,723 + -1.4 115,723 + -1.0 115,724 - +1.2 115,736 + -3.8 115,736 + -2.6 115,882 - -0.8 115,902 + -0.2 115,902 + -0.8 115,902 + -1.8 115,948 + -0.5 115,948 + -1.9 115,952 + -0.2 115,952 + -2.3 115,952 + -1.0 115,952 + -1.2 115,952 + -1.7 115,952 + -1.4 115,952 + -0.9 115,953 - +0.0 115,976 + -2.3 115,976 + -0.8 115,976 + -2.0 115,976 + +0.2 115,989 + -1.3 115,989 + -1.7 116,000 + -1.7 116,000 + -1.4 116,000 + -2.3 116,001 - +0.4 116,036 + -2.2 116,036 + -2.0 116,036 + -1.3 116,036 + -1.7 116,037 - +0.2 116,037 - -0.0 116,093 + -2.0 116,093 + -1.1 116,093 + -2.3 116,093 + -1.9 116,093 + -1.4 116,093 + -2.5 116,094 - -0.3 116,095 + -1.2 116,095 + -0.6 116,095 + -1.7 116,095 + -0.5 116,095 + -2.3 116,095 + -2.0 116,165 - +0.2 116,188 + -3.2 116,208 + -2.1
Or see this region's nucleotide sequence