Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5352 and Pf1N1B4_5353 are separated by 516 nucleotides Pf1N1B4_5353 and Pf1N1B4_5354 are separated by 109 nucleotides Pf1N1B4_5354 and Pf1N1B4_5355 are separated by 17 nucleotides Pf1N1B4_5355 and Pf1N1B4_5356 are separated by 360 nucleotides
Pf1N1B4_5352 - hypothetical protein, at 91,210 to 91,479
_5352
Pf1N1B4_5353 - hypothetical protein, at 91,996 to 92,163
_5353
Pf1N1B4_5354 - hypothetical protein, at 92,273 to 92,416
_5354
Pf1N1B4_5355 - hypothetical protein, at 92,434 to 92,616
_5355
Pf1N1B4_5356 - hypothetical protein, at 92,977 to 93,222
_5356
Position (kb)
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 91.493 kb on + strand at 91.493 kb on + strand at 91.494 kb on - strand at 91.523 kb on + strand at 91.523 kb on + strand at 91.524 kb on - strand at 91.567 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.792 kb on + strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.872 kb on - strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.892 kb on - strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.908 kb on - strand at 91.908 kb on - strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.930 kb on - strand at 91.930 kb on - strand at 91.950 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.994 kb on - strand at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.161 kb on + strand at 92.165 kb on + strand at 92.165 kb on + strand at 92.186 kb on + strand at 92.302 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.339 kb on - strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.385 kb on + strand, within Pf1N1B4_5354 at 92.443 kb on + strand at 92.443 kb on + strand at 92.496 kb on + strand, within Pf1N1B4_5355 at 92.496 kb on + strand at 92.497 kb on - strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.626 kb on + strand at 92.626 kb on + strand at 92.658 kb on + strand at 92.658 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.678 kb on - strand at 92.678 kb on - strand at 92.871 kb on + strand at 92.871 kb on + strand at 92.872 kb on - strand at 92.872 kb on - strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.966 kb on - strand at 92.995 kb on + strand at 92.996 kb on - strand at 92.996 kb on - strand at 93.001 kb on + strand at 93.001 kb on + strand at 93.036 kb on + strand, within Pf1N1B4_5356 at 93.114 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.185 kb on + strand at 93.186 kb on - strand, within Pf1N1B4_5356 at 93.220 kb on + strand at 93.283 kb on - strand at 93.315 kb on + strand at 93.315 kb on + strand at 93.315 kb on + strand at 93.316 kb on - strand at 93.466 kb on - strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.530 kb on - strand at 93.530 kb on - strand at 93.530 kb on - strand at 93.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 91,493 + +1.1 91,493 + +0.6 91,494 - +0.8 91,523 + +1.7 91,523 + -2.8 91,524 - +0.7 91,567 + -1.0 91,625 + -2.5 91,625 + +1.9 91,625 + -0.7 91,626 - +1.4 91,626 - -2.7 91,626 - +0.0 91,792 + +1.6 91,793 - -0.5 91,793 - -1.5 91,793 - -0.8 91,794 + +1.3 91,794 + +1.2 91,794 + +2.8 91,794 + +1.1 91,794 + +0.8 91,794 + +1.8 91,871 + -0.1 91,871 + -0.7 91,871 + +2.0 91,871 + -0.5 91,871 + +2.2 91,871 + +0.8 91,871 + -0.1 91,871 + +2.6 91,871 + +0.3 91,872 - -0.8 91,891 + +1.6 91,891 + -0.2 91,891 + +2.1 91,891 + +0.7 91,891 + +1.5 91,891 + +1.4 91,892 - +0.3 91,907 + +0.5 91,907 + +1.2 91,907 + +1.3 91,907 + +2.4 91,907 + +0.1 91,908 - -0.4 91,908 - +0.7 91,917 + +2.1 91,917 + +1.1 91,917 + +0.5 91,917 + +0.3 91,917 + +1.6 91,917 + -0.7 91,917 + +1.5 91,929 + +3.1 91,929 + +1.4 91,929 + +2.2 91,929 + +0.6 91,929 + +2.2 91,929 + +0.7 91,929 + +1.6 91,929 + +2.2 91,929 + +1.9 91,930 - -0.2 91,930 - -3.1 91,950 + +1.4 91,993 + -2.0 91,993 + +0.2 91,993 + +2.6 91,993 + +0.5 91,994 - -2.0 92,092 + Pf1N1B4_5353 0.57 +1.8 92,092 + Pf1N1B4_5353 0.57 +1.3 92,092 + Pf1N1B4_5353 0.57 +0.9 92,092 + Pf1N1B4_5353 0.57 +0.1 92,092 + Pf1N1B4_5353 0.57 +0.8 92,092 + Pf1N1B4_5353 0.57 +0.2 92,092 + Pf1N1B4_5353 0.57 +1.3 92,093 - Pf1N1B4_5353 0.58 -1.1 92,093 - Pf1N1B4_5353 0.58 -2.4 92,161 + +1.7 92,165 + +1.6 92,165 + +0.4 92,186 + +1.3 92,302 + Pf1N1B4_5354 0.20 +0.4 92,338 + Pf1N1B4_5354 0.45 +0.4 92,338 + Pf1N1B4_5354 0.45 +1.4 92,338 + Pf1N1B4_5354 0.45 +0.0 92,339 - Pf1N1B4_5354 0.46 -0.7 92,349 + Pf1N1B4_5354 0.53 +1.0 92,349 + Pf1N1B4_5354 0.53 +1.2 92,385 + Pf1N1B4_5354 0.78 +1.6 92,443 + +0.1 92,443 + +0.6 92,496 + Pf1N1B4_5355 0.34 +0.9 92,496 + +2.8 92,497 - Pf1N1B4_5355 0.34 -0.8 92,500 + Pf1N1B4_5355 0.36 +3.3 92,500 + Pf1N1B4_5355 0.36 +2.1 92,501 - Pf1N1B4_5355 0.37 +0.4 92,501 - Pf1N1B4_5355 0.37 -0.8 92,539 + Pf1N1B4_5355 0.57 +1.0 92,539 + Pf1N1B4_5355 0.57 +0.2 92,539 + Pf1N1B4_5355 0.57 +3.4 92,539 + Pf1N1B4_5355 0.57 +0.3 92,539 + Pf1N1B4_5355 0.57 +1.2 92,539 + Pf1N1B4_5355 0.57 +2.8 92,539 + Pf1N1B4_5355 0.57 +1.1 92,539 + Pf1N1B4_5355 0.57 +1.5 92,539 + Pf1N1B4_5355 0.57 +1.6 92,539 + Pf1N1B4_5355 0.57 +1.3 92,539 + Pf1N1B4_5355 0.57 +2.3 92,541 + Pf1N1B4_5355 0.58 -3.1 92,541 + Pf1N1B4_5355 0.58 +1.5 92,541 + Pf1N1B4_5355 0.58 +1.3 92,626 + +1.3 92,626 + +1.2 92,658 + -0.2 92,658 + +0.6 92,677 + +0.8 92,677 + +0.3 92,677 + -1.5 92,678 - -1.8 92,678 - +0.1 92,871 + +0.4 92,871 + -0.2 92,872 - -0.8 92,872 - -1.8 92,934 + -0.5 92,934 + -0.8 92,934 + -0.2 92,934 + -0.5 92,934 + -0.4 92,935 - +0.8 92,935 - -2.5 92,935 - -2.0 92,935 - -0.0 92,935 - +0.0 92,935 - +0.5 92,935 - +2.9 92,935 - -1.8 92,935 - -2.0 92,937 + +0.8 92,937 + -0.8 92,937 + +0.2 92,937 + -1.0 92,937 + -2.0 92,937 + +0.4 92,937 + -0.0 92,937 + -0.4 92,937 + -0.5 92,938 - +1.7 92,938 - -1.2 92,938 - +0.4 92,939 + +0.2 92,939 + -0.8 92,939 + +1.2 92,955 - -1.1 92,955 - -1.5 92,955 - +0.2 92,966 - +0.5 92,995 + -1.2 92,996 - -0.5 92,996 - -0.8 93,001 + -0.4 93,001 + -2.0 93,036 + Pf1N1B4_5356 0.24 -0.1 93,114 + Pf1N1B4_5356 0.56 +0.9 93,147 + Pf1N1B4_5356 0.69 -0.5 93,147 + Pf1N1B4_5356 0.69 -2.2 93,185 + -1.2 93,186 - Pf1N1B4_5356 0.85 +1.7 93,220 + -0.5 93,283 - +0.4 93,315 + +0.5 93,315 + +0.4 93,315 + -2.5 93,316 - +0.7 93,466 - +1.0 93,529 + +1.1 93,529 + -1.2 93,529 + -1.5 93,529 + -1.3 93,529 + +1.4 93,529 + +1.1 93,529 + +0.2 93,529 + +0.2 93,529 + +0.5 93,529 + +0.8 93,529 + -0.5 93,529 + +1.4 93,529 + -2.2 93,529 + +2.0 93,529 + -2.2 93,529 + +0.5 93,529 + -1.5 93,530 - -0.5 93,530 - +0.6 93,530 - -1.9 93,530 - +1.0
Or see this region's nucleotide sequence