Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2908 and Pf1N1B4_5209 are separated by 167 nucleotides Pf1N1B4_5209 and Pf1N1B4_2909 are separated by 116 nucleotides
Pf1N1B4_2908 - Integrase, at 3,263,136 to 3,263,828
_2908
Pf1N1B4_5209 - tRNA-Met-CAT, at 3,263,996 to 3,264,069
_5209
Pf1N1B4_2909 - Methyl-accepting chemotaxis protein, at 3,264,186 to 3,266,243
_2909
Position (kb)
3263
3264
3265 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3263.056 kb on + strand at 3263.056 kb on + strand at 3263.057 kb on - strand at 3263.057 kb on - strand at 3263.057 kb on - strand at 3263.096 kb on + strand at 3263.097 kb on - strand at 3263.137 kb on + strand at 3263.191 kb on + strand at 3263.192 kb on - strand at 3263.278 kb on + strand, within Pf1N1B4_2908 at 3263.279 kb on - strand, within Pf1N1B4_2908 at 3263.279 kb on - strand at 3263.293 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.367 kb on - strand, within Pf1N1B4_2908 at 3263.367 kb on - strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.383 kb on + strand, within Pf1N1B4_2908 at 3263.384 kb on - strand, within Pf1N1B4_2908 at 3263.523 kb on + strand at 3263.523 kb on + strand, within Pf1N1B4_2908 at 3263.523 kb on + strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.589 kb on + strand, within Pf1N1B4_2908 at 3263.589 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.680 kb on + strand, within Pf1N1B4_2908 at 3263.680 kb on + strand, within Pf1N1B4_2908 at 3263.681 kb on - strand, within Pf1N1B4_2908 at 3263.708 kb on + strand, within Pf1N1B4_2908 at 3263.709 kb on - strand, within Pf1N1B4_2908 at 3263.725 kb on + strand, within Pf1N1B4_2908 at 3263.725 kb on + strand, within Pf1N1B4_2908 at 3263.726 kb on - strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.743 kb on - strand at 3263.752 kb on + strand, within Pf1N1B4_2908 at 3263.752 kb on + strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.781 kb on + strand at 3263.782 kb on - strand at 3263.834 kb on + strand at 3263.835 kb on - strand at 3263.835 kb on - strand at 3263.855 kb on - strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.876 kb on - strand at 3263.915 kb on + strand at 3263.915 kb on + strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.919 kb on + strand at 3263.919 kb on + strand at 3263.919 kb on + strand at 3263.962 kb on - strand at 3263.962 kb on - strand at 3263.962 kb on - strand at 3264.125 kb on - strand at 3264.228 kb on - strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.776 kb on + strand, within Pf1N1B4_2909 at 3264.777 kb on - strand, within Pf1N1B4_2909
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,263,056 + -1.3 3,263,056 + +1.0 3,263,057 - -2.8 3,263,057 - -0.8 3,263,057 - -0.3 3,263,096 + -0.8 3,263,097 - -1.3 3,263,137 + -0.3 3,263,191 + -0.4 3,263,192 - +2.7 3,263,278 + Pf1N1B4_2908 0.20 +0.2 3,263,279 - Pf1N1B4_2908 0.21 -1.1 3,263,279 - -0.8 3,263,293 + Pf1N1B4_2908 0.23 +1.2 3,263,366 + Pf1N1B4_2908 0.33 +0.7 3,263,366 + Pf1N1B4_2908 0.33 +0.5 3,263,366 + Pf1N1B4_2908 0.33 +0.2 3,263,366 + Pf1N1B4_2908 0.33 -1.1 3,263,366 + Pf1N1B4_2908 0.33 -0.1 3,263,367 - Pf1N1B4_2908 0.33 -1.1 3,263,367 - Pf1N1B4_2908 0.33 +0.9 3,263,370 + Pf1N1B4_2908 0.34 +0.0 3,263,370 + Pf1N1B4_2908 0.34 -0.6 3,263,370 + Pf1N1B4_2908 0.34 +2.2 3,263,370 + Pf1N1B4_2908 0.34 -0.1 3,263,370 + Pf1N1B4_2908 0.34 -1.1 3,263,370 + Pf1N1B4_2908 0.34 -0.3 3,263,371 - Pf1N1B4_2908 0.34 -1.6 3,263,371 - Pf1N1B4_2908 0.34 +0.6 3,263,371 - Pf1N1B4_2908 0.34 +0.2 3,263,371 - Pf1N1B4_2908 0.34 -1.1 3,263,371 - Pf1N1B4_2908 0.34 +0.0 3,263,371 - Pf1N1B4_2908 0.34 -1.1 3,263,383 + Pf1N1B4_2908 0.36 -0.6 3,263,384 - Pf1N1B4_2908 0.36 +0.2 3,263,523 + -0.3 3,263,523 + Pf1N1B4_2908 0.56 -2.3 3,263,523 + Pf1N1B4_2908 0.56 -0.5 3,263,524 - Pf1N1B4_2908 0.56 -1.3 3,263,524 - Pf1N1B4_2908 0.56 -0.3 3,263,524 - Pf1N1B4_2908 0.56 -0.3 3,263,524 - Pf1N1B4_2908 0.56 -1.8 3,263,524 - Pf1N1B4_2908 0.56 -0.3 3,263,524 - Pf1N1B4_2908 0.56 -1.6 3,263,541 - Pf1N1B4_2908 0.58 -0.6 3,263,541 - Pf1N1B4_2908 0.58 +0.2 3,263,541 - Pf1N1B4_2908 0.58 -1.1 3,263,589 + Pf1N1B4_2908 0.65 -0.2 3,263,589 + Pf1N1B4_2908 0.65 +0.9 3,263,678 + Pf1N1B4_2908 0.78 -1.1 3,263,678 + Pf1N1B4_2908 0.78 +1.4 3,263,678 + Pf1N1B4_2908 0.78 +1.1 3,263,678 + Pf1N1B4_2908 0.78 -1.2 3,263,680 + Pf1N1B4_2908 0.78 -0.3 3,263,680 + Pf1N1B4_2908 0.78 +0.1 3,263,681 - Pf1N1B4_2908 0.79 -1.6 3,263,708 + Pf1N1B4_2908 0.83 +1.6 3,263,709 - Pf1N1B4_2908 0.83 +0.4 3,263,725 + Pf1N1B4_2908 0.85 +0.7 3,263,725 + Pf1N1B4_2908 0.85 +1.7 3,263,726 - Pf1N1B4_2908 0.85 +1.2 3,263,742 + Pf1N1B4_2908 0.87 +0.2 3,263,742 + Pf1N1B4_2908 0.87 +2.1 3,263,742 + Pf1N1B4_2908 0.87 +3.1 3,263,743 - +2.8 3,263,752 + Pf1N1B4_2908 0.89 -0.9 3,263,752 + Pf1N1B4_2908 0.89 +1.2 3,263,753 - Pf1N1B4_2908 0.89 +0.7 3,263,753 - Pf1N1B4_2908 0.89 +2.2 3,263,753 - -0.8 3,263,753 - Pf1N1B4_2908 0.89 +0.1 3,263,753 - Pf1N1B4_2908 0.89 +0.1 3,263,781 + -0.3 3,263,782 - -0.9 3,263,834 + +1.4 3,263,835 - +0.5 3,263,835 - +1.1 3,263,855 - +2.4 3,263,864 + +0.2 3,263,864 + -1.6 3,263,864 + +0.4 3,263,864 + -0.3 3,263,864 + +1.7 3,263,864 + +1.2 3,263,864 + -2.1 3,263,864 + -1.8 3,263,864 + -0.3 3,263,864 + -2.8 3,263,864 + -0.6 3,263,864 + -0.3 3,263,864 + +0.2 3,263,865 - +2.8 3,263,865 - -1.1 3,263,865 - -1.3 3,263,865 - -2.3 3,263,865 - -0.9 3,263,865 - +0.2 3,263,865 - +3.1 3,263,865 - -1.7 3,263,865 - -1.6 3,263,865 - -0.8 3,263,865 - -0.8 3,263,865 - -0.8 3,263,876 - -0.8 3,263,915 + -0.8 3,263,915 + +0.6 3,263,916 - -0.8 3,263,916 - +0.0 3,263,916 - -1.1 3,263,916 - -0.3 3,263,919 + +1.2 3,263,919 + -0.8 3,263,919 + +1.2 3,263,962 - -1.9 3,263,962 - -0.8 3,263,962 - +3.8 3,264,125 - +0.9 3,264,228 - -0.8 3,264,282 + -0.1 3,264,282 + +0.8 3,264,282 + -0.5 3,264,282 + +2.0 3,264,282 + -0.1 3,264,282 + +0.1 3,264,282 + +1.2 3,264,282 + -1.1 3,264,283 - -1.6 3,264,283 - -1.1 3,264,283 - -1.0 3,264,283 - -1.7 3,264,283 - +1.1 3,264,776 + Pf1N1B4_2909 0.29 +0.5 3,264,777 - Pf1N1B4_2909 0.29 -0.3
Or see this region's nucleotide sequence