Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5044 and Pf1N1B4_5045 are separated by 278 nucleotides Pf1N1B4_5045 and Pf1N1B4_5046 overlap by 1 nucleotides Pf1N1B4_5046 and Pf1N1B4_5047 are separated by 184 nucleotides
Pf1N1B4_5044 - hypothetical protein, at 5,589,635 to 5,589,994
_5044
Pf1N1B4_5045 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone, at 5,590,273 to 5,590,878
_5045
Pf1N1B4_5046 - Copper metallochaperone, bacterial analog of Cox17 protein, at 5,590,878 to 5,591,351
_5046
Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507
_5047
Position (kb)
5590
5591 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5589.299 kb on - strand at 5589.299 kb on - strand at 5589.328 kb on + strand at 5589.329 kb on - strand at 5589.450 kb on + strand at 5589.548 kb on - strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.635 kb on - strand at 5589.635 kb on - strand at 5589.775 kb on + strand at 5589.775 kb on + strand at 5589.979 kb on + strand at 5589.979 kb on + strand at 5589.979 kb on + strand at 5589.982 kb on + strand at 5589.983 kb on - strand at 5589.992 kb on + strand at 5589.993 kb on - strand at 5589.993 kb on - strand at 5590.253 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.286 kb on - strand at 5590.286 kb on - strand at 5590.376 kb on - strand, within Pf1N1B4_5045 at 5590.411 kb on - strand at 5590.672 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.902 kb on - strand at 5590.904 kb on + strand at 5590.905 kb on - strand at 5590.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,589,299 - +1.5 5,589,299 - -1.2 5,589,328 + +0.9 5,589,329 - +1.9 5,589,450 + +2.3 5,589,548 - -1.3 5,589,634 + -2.2 5,589,634 + +0.0 5,589,634 + +1.8 5,589,635 - -0.4 5,589,635 - +0.4 5,589,775 + +0.8 5,589,775 + -0.8 5,589,979 + +1.3 5,589,979 + -1.6 5,589,979 + +1.5 5,589,982 + -0.2 5,589,983 - -2.6 5,589,992 + +1.3 5,589,993 - +1.8 5,589,993 - -0.8 5,590,253 + -0.8 5,590,278 + -1.8 5,590,278 + +0.3 5,590,278 + -0.3 5,590,278 + +2.1 5,590,278 + +0.8 5,590,278 + -1.2 5,590,278 + +1.0 5,590,279 - -0.8 5,590,279 - +0.2 5,590,279 - +0.2 5,590,279 - +0.8 5,590,279 - +1.3 5,590,279 - -1.2 5,590,285 + -0.1 5,590,285 + +0.2 5,590,285 + -2.7 5,590,285 + +0.4 5,590,285 + +0.1 5,590,285 + -1.6 5,590,286 - +1.3 5,590,286 - +1.8 5,590,376 - Pf1N1B4_5045 0.17 -1.6 5,590,411 - -0.2 5,590,672 + Pf1N1B4_5045 0.66 -1.2 5,590,750 + Pf1N1B4_5045 0.79 -1.8 5,590,750 + Pf1N1B4_5045 0.79 -1.8 5,590,750 + Pf1N1B4_5045 0.79 -0.2 5,590,750 + Pf1N1B4_5045 0.79 +0.2 5,590,750 + -0.8 5,590,751 - Pf1N1B4_5045 0.79 -0.8 5,590,751 - Pf1N1B4_5045 0.79 -2.6 5,590,751 - Pf1N1B4_5045 0.79 -1.8 5,590,890 + +0.8 5,590,890 + +1.2 5,590,890 + +0.6 5,590,891 - -0.0 5,590,891 - -0.8 5,590,891 - +2.1 5,590,901 + -1.6 5,590,901 + +1.5 5,590,901 + +0.3 5,590,901 + -1.0 5,590,901 + +1.0 5,590,901 + +1.2 5,590,902 - +2.2 5,590,904 + -2.6 5,590,905 - +0.0 5,590,905 - -0.5
Or see this region's nucleotide sequence