Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4910

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4909 and Pf1N1B4_4910 are separated by 236 nucleotidesPf1N1B4_4910 and Pf1N1B4_4911 are separated by 95 nucleotidesPf1N1B4_4911 and Pf1N1B4_4912 are separated by 45 nucleotides Pf1N1B4_4909 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,438,861 to 5,439,823 _4909 Pf1N1B4_4910 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 5,440,060 to 5,441,352 _4910 Pf1N1B4_4911 - FIG00953249: hypothetical protein, at 5,441,448 to 5,442,104 _4911 Pf1N1B4_4912 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,442,150 to 5,442,638 _4912 Position (kb) 5440 5441 5442Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5439.495 kb on + strand, within Pf1N1B4_4909at 5439.663 kb on + strandat 5439.772 kb on + strandat 5439.773 kb on - strandat 5439.775 kb on - strandat 5439.876 kb on + strandat 5439.982 kb on + strandat 5440.319 kb on - strand, within Pf1N1B4_4910at 5440.327 kb on + strand, within Pf1N1B4_4910at 5440.380 kb on + strand, within Pf1N1B4_4910at 5440.534 kb on + strand, within Pf1N1B4_4910at 5440.534 kb on + strand, within Pf1N1B4_4910at 5440.536 kb on + strand, within Pf1N1B4_4910at 5440.536 kb on + strand, within Pf1N1B4_4910at 5440.537 kb on - strandat 5440.963 kb on + strand, within Pf1N1B4_4910at 5440.963 kb on + strand, within Pf1N1B4_4910at 5440.963 kb on + strand, within Pf1N1B4_4910at 5440.964 kb on - strand, within Pf1N1B4_4910at 5440.964 kb on - strand, within Pf1N1B4_4910at 5441.402 kb on - strandat 5441.402 kb on - strandat 5441.441 kb on - strandat 5441.441 kb on - strandat 5441.441 kb on - strandat 5442.045 kb on + strandat 5442.125 kb on - strandat 5442.125 kb on - strandat 5442.161 kb on - strandat 5442.164 kb on - strandat 5442.303 kb on + strand, within Pf1N1B4_4912at 5442.306 kb on - strand, within Pf1N1B4_4912at 5442.316 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,439,495 + Pf1N1B4_4909 0.66 +0.6
5,439,663 + +1.8
5,439,772 + -0.2
5,439,773 - -0.6
5,439,775 - +0.8
5,439,876 + +1.8
5,439,982 + +0.8
5,440,319 - Pf1N1B4_4910 0.20 +1.0
5,440,327 + Pf1N1B4_4910 0.21 +0.6
5,440,380 + Pf1N1B4_4910 0.25 -0.2
5,440,534 + Pf1N1B4_4910 0.37 -0.5
5,440,534 + Pf1N1B4_4910 0.37 +2.4
5,440,536 + Pf1N1B4_4910 0.37 -0.7
5,440,536 + Pf1N1B4_4910 0.37 +0.0
5,440,537 - -0.2
5,440,963 + Pf1N1B4_4910 0.70 +0.2
5,440,963 + Pf1N1B4_4910 0.70 -1.8
5,440,963 + Pf1N1B4_4910 0.70 +0.6
5,440,964 - Pf1N1B4_4910 0.70 -0.2
5,440,964 - Pf1N1B4_4910 0.70 -0.8
5,441,402 - -1.2
5,441,402 - +3.9
5,441,441 - -2.0
5,441,441 - -1.8
5,441,441 - -1.2
5,442,045 + +1.2
5,442,125 - -0.8
5,442,125 - -0.5
5,442,161 - +0.8
5,442,164 - -0.6
5,442,303 + Pf1N1B4_4912 0.31 +0.4
5,442,306 - Pf1N1B4_4912 0.32 +0.4
5,442,316 - -0.2

Or see this region's nucleotide sequence