Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4909 and Pf1N1B4_4910 are separated by 236 nucleotides Pf1N1B4_4910 and Pf1N1B4_4911 are separated by 95 nucleotides Pf1N1B4_4911 and Pf1N1B4_4912 are separated by 45 nucleotides
Pf1N1B4_4909 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,438,861 to 5,439,823
_4909
Pf1N1B4_4910 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 5,440,060 to 5,441,352
_4910
Pf1N1B4_4911 - FIG00953249: hypothetical protein, at 5,441,448 to 5,442,104
_4911
Pf1N1B4_4912 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,442,150 to 5,442,638
_4912
Position (kb)
5440
5441
5442 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5439.495 kb on + strand, within Pf1N1B4_4909 at 5439.663 kb on + strand at 5439.772 kb on + strand at 5439.773 kb on - strand at 5439.775 kb on - strand at 5439.876 kb on + strand at 5439.982 kb on + strand at 5440.319 kb on - strand, within Pf1N1B4_4910 at 5440.327 kb on + strand, within Pf1N1B4_4910 at 5440.380 kb on + strand, within Pf1N1B4_4910 at 5440.534 kb on + strand, within Pf1N1B4_4910 at 5440.534 kb on + strand, within Pf1N1B4_4910 at 5440.536 kb on + strand, within Pf1N1B4_4910 at 5440.536 kb on + strand, within Pf1N1B4_4910 at 5440.537 kb on - strand at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.964 kb on - strand, within Pf1N1B4_4910 at 5440.964 kb on - strand, within Pf1N1B4_4910 at 5441.402 kb on - strand at 5441.402 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5442.045 kb on + strand at 5442.125 kb on - strand at 5442.125 kb on - strand at 5442.161 kb on - strand at 5442.164 kb on - strand at 5442.303 kb on + strand, within Pf1N1B4_4912 at 5442.306 kb on - strand, within Pf1N1B4_4912 at 5442.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,439,495 + Pf1N1B4_4909 0.66 +0.6 5,439,663 + +1.8 5,439,772 + -0.2 5,439,773 - -0.6 5,439,775 - +0.8 5,439,876 + +1.8 5,439,982 + +0.8 5,440,319 - Pf1N1B4_4910 0.20 +1.0 5,440,327 + Pf1N1B4_4910 0.21 +0.6 5,440,380 + Pf1N1B4_4910 0.25 -0.2 5,440,534 + Pf1N1B4_4910 0.37 -0.5 5,440,534 + Pf1N1B4_4910 0.37 +2.4 5,440,536 + Pf1N1B4_4910 0.37 -0.7 5,440,536 + Pf1N1B4_4910 0.37 +0.0 5,440,537 - -0.2 5,440,963 + Pf1N1B4_4910 0.70 +0.2 5,440,963 + Pf1N1B4_4910 0.70 -1.8 5,440,963 + Pf1N1B4_4910 0.70 +0.6 5,440,964 - Pf1N1B4_4910 0.70 -0.2 5,440,964 - Pf1N1B4_4910 0.70 -0.8 5,441,402 - -1.2 5,441,402 - +3.9 5,441,441 - -2.0 5,441,441 - -1.8 5,441,441 - -1.2 5,442,045 + +1.2 5,442,125 - -0.8 5,442,125 - -0.5 5,442,161 - +0.8 5,442,164 - -0.6 5,442,303 + Pf1N1B4_4912 0.31 +0.4 5,442,306 - Pf1N1B4_4912 0.32 +0.4 5,442,316 - -0.2
Or see this region's nucleotide sequence