Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4869 and Pf1N1B4_4870 are separated by 12 nucleotides Pf1N1B4_4870 and Pf1N1B4_4871 are separated by 109 nucleotides Pf1N1B4_4871 and Pf1N1B4_4872 are separated by 80 nucleotides Pf1N1B4_4872 and Pf1N1B4_4873 are separated by 37 nucleotides
Pf1N1B4_4869 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,388,805 to 5,391,045
_4869
Pf1N1B4_4870 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,391,058 to 5,391,531
_4870
Pf1N1B4_4871 - Aldo-keto reductase, at 5,391,641 to 5,391,895
_4871
Pf1N1B4_4872 - Short-chain dehydrogenase/reductase SDR, at 5,391,976 to 5,392,818
_4872
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Position (kb)
5391
5392 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5390.686 kb on - strand, within Pf1N1B4_4869 at 5390.927 kb on - strand at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.996 kb on - strand at 5390.996 kb on - strand at 5391.271 kb on + strand, within Pf1N1B4_4870 at 5391.271 kb on + strand at 5391.393 kb on + strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.487 kb on + strand at 5391.500 kb on + strand at 5391.501 kb on - strand at 5391.596 kb on + strand at 5391.597 kb on - strand at 5391.727 kb on + strand, within Pf1N1B4_4871 at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.948 kb on - strand at 5391.951 kb on + strand at 5392.028 kb on + strand at 5392.028 kb on + strand at 5392.490 kb on + strand, within Pf1N1B4_4872 at 5392.555 kb on + strand, within Pf1N1B4_4872 at 5392.556 kb on - strand, within Pf1N1B4_4872 at 5392.579 kb on + strand, within Pf1N1B4_4872 at 5392.580 kb on - strand, within Pf1N1B4_4872 at 5392.782 kb on + strand at 5392.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,390,686 - Pf1N1B4_4869 0.84 +1.3 5,390,927 - -2.3 5,390,995 + -0.2 5,390,995 + +0.3 5,390,995 + -1.5 5,390,996 - -0.9 5,390,996 - -2.5 5,391,271 + Pf1N1B4_4870 0.45 +2.4 5,391,271 + +1.2 5,391,393 + Pf1N1B4_4870 0.71 -1.8 5,391,394 - Pf1N1B4_4870 0.71 +0.3 5,391,394 - Pf1N1B4_4870 0.71 -1.8 5,391,487 + +0.8 5,391,500 + -0.2 5,391,501 - -0.2 5,391,596 + +0.8 5,391,597 - +0.2 5,391,727 + Pf1N1B4_4871 0.34 +2.2 5,391,947 + +1.0 5,391,947 + -1.2 5,391,947 + -0.1 5,391,947 + +0.3 5,391,947 + +0.3 5,391,948 - -2.0 5,391,951 + -1.0 5,392,028 + -1.2 5,392,028 + +1.2 5,392,490 + Pf1N1B4_4872 0.61 +1.1 5,392,555 + Pf1N1B4_4872 0.69 -0.5 5,392,556 - Pf1N1B4_4872 0.69 +0.8 5,392,579 + Pf1N1B4_4872 0.72 +0.9 5,392,580 - Pf1N1B4_4872 0.72 +1.5 5,392,782 + +0.1 5,392,782 + +1.2
Or see this region's nucleotide sequence