Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4862 and Pf1N1B4_4863 are separated by 338 nucleotides Pf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotides
Pf1N1B4_4862 - TPR domain protein, at 5,376,951 to 5,378,006
_4862
Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766
_4863
Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954
_4864
Position (kb) 5378
5379
5380 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5377.888 kb on + strand at 5377.889 kb on - strand, within Pf1N1B4_4862 at 5378.174 kb on - strand at 5378.174 kb on - strand at 5378.255 kb on + strand at 5378.255 kb on + strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.346 kb on + strand at 5378.346 kb on + strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.394 kb on + strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.650 kb on - strand at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5379.232 kb on - strand, within Pf1N1B4_4863 at 5379.379 kb on - strand, within Pf1N1B4_4863 at 5379.851 kb on - strand at 5379.851 kb on - strand at 5379.854 kb on + strand at 5379.854 kb on + strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5380.110 kb on + strand, within Pf1N1B4_4864 at 5380.111 kb on - strand, within Pf1N1B4_4864 at 5380.111 kb on - strand at 5380.254 kb on - strand, within Pf1N1B4_4864 at 5380.286 kb on - strand, within Pf1N1B4_4864 at 5380.337 kb on + strand at 5380.338 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.658 kb on + strand, within Pf1N1B4_4864 at 5380.673 kb on + strand, within Pf1N1B4_4864
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,377,888 + -0.2 5,377,889 - Pf1N1B4_4862 0.89 +0.8 5,378,174 - +1.8 5,378,174 - +1.1 5,378,255 + -2.8 5,378,255 + +1.8 5,378,256 - -1.2 5,378,256 - +3.1 5,378,256 - +1.8 5,378,256 - +0.3 5,378,256 - +0.2 5,378,256 - +1.0 5,378,256 - +1.1 5,378,256 - +0.8 5,378,256 - +0.8 5,378,346 + -0.0 5,378,346 + -1.1 5,378,347 - +2.0 5,378,347 - +1.5 5,378,347 - +0.2 5,378,394 + +0.8 5,378,395 - +0.0 5,378,395 - +1.4 5,378,395 - +0.5 5,378,395 - -0.1 5,378,649 + Pf1N1B4_4863 0.21 -0.8 5,378,649 + Pf1N1B4_4863 0.21 +1.9 5,378,649 + Pf1N1B4_4863 0.21 +0.3 5,378,650 - -0.2 5,378,650 - Pf1N1B4_4863 0.21 +0.8 5,378,650 - Pf1N1B4_4863 0.21 -1.7 5,378,650 - Pf1N1B4_4863 0.21 -0.0 5,378,650 - Pf1N1B4_4863 0.21 +1.1 5,378,650 - Pf1N1B4_4863 0.21 +0.1 5,378,650 - Pf1N1B4_4863 0.21 +1.3 5,378,650 - Pf1N1B4_4863 0.21 -1.0 5,378,732 - Pf1N1B4_4863 0.27 -0.4 5,378,732 - Pf1N1B4_4863 0.27 +0.2 5,378,732 - Pf1N1B4_4863 0.27 +1.5 5,379,232 - Pf1N1B4_4863 0.62 -0.2 5,379,379 - Pf1N1B4_4863 0.73 -1.2 5,379,851 - -1.6 5,379,851 - -2.4 5,379,854 + -0.2 5,379,854 + -2.0 5,379,855 - +0.2 5,379,855 - -0.4 5,379,855 - -0.6 5,379,855 - +0.1 5,379,855 - -0.8 5,379,855 - -1.9 5,379,855 - -0.3 5,380,110 + Pf1N1B4_4864 0.11 -1.2 5,380,111 - Pf1N1B4_4864 0.11 -3.3 5,380,111 - -1.2 5,380,254 - Pf1N1B4_4864 0.15 -1.8 5,380,286 - Pf1N1B4_4864 0.16 -2.2 5,380,337 + -0.8 5,380,338 - Pf1N1B4_4864 0.18 -2.9 5,380,476 - Pf1N1B4_4864 0.22 -2.0 5,380,476 - Pf1N1B4_4864 0.22 -1.8 5,380,476 - Pf1N1B4_4864 0.22 -1.2 5,380,476 - Pf1N1B4_4864 0.22 -0.5 5,380,625 + Pf1N1B4_4864 0.27 -1.5 5,380,625 + Pf1N1B4_4864 0.27 -0.7 5,380,658 + Pf1N1B4_4864 0.28 +0.8 5,380,673 + Pf1N1B4_4864 0.29 -0.5
Or see this region's nucleotide sequence