Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4861 and Pf1N1B4_4862 are separated by 257 nucleotides Pf1N1B4_4862 and Pf1N1B4_4863 are separated by 338 nucleotides
Pf1N1B4_4861 - KaiC, at 5,375,191 to 5,376,693
_4861
Pf1N1B4_4862 - TPR domain protein, at 5,376,951 to 5,378,006
_4862
Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766
_4863
Position (kb) 5376
5377
5378
5379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5376.206 kb on + strand, within Pf1N1B4_4861 at 5376.207 kb on - strand, within Pf1N1B4_4861 at 5376.365 kb on + strand, within Pf1N1B4_4861 at 5376.365 kb on + strand, within Pf1N1B4_4861 at 5376.507 kb on - strand, within Pf1N1B4_4861 at 5376.687 kb on + strand at 5376.812 kb on + strand at 5376.812 kb on + strand at 5376.813 kb on - strand at 5377.049 kb on - strand at 5377.888 kb on + strand at 5377.889 kb on - strand, within Pf1N1B4_4862 at 5378.174 kb on - strand at 5378.174 kb on - strand at 5378.255 kb on + strand at 5378.255 kb on + strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.346 kb on + strand at 5378.346 kb on + strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.394 kb on + strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.650 kb on - strand at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,376,206 + Pf1N1B4_4861 0.68 +0.8 5,376,207 - Pf1N1B4_4861 0.68 +0.5 5,376,365 + Pf1N1B4_4861 0.78 +0.8 5,376,365 + Pf1N1B4_4861 0.78 +1.1 5,376,507 - Pf1N1B4_4861 0.88 +0.0 5,376,687 + -0.2 5,376,812 + +0.2 5,376,812 + -1.2 5,376,813 - +0.8 5,377,049 - -1.8 5,377,888 + -0.2 5,377,889 - Pf1N1B4_4862 0.89 +0.8 5,378,174 - +1.8 5,378,174 - +1.1 5,378,255 + -2.8 5,378,255 + +1.8 5,378,256 - -1.2 5,378,256 - +3.1 5,378,256 - +1.8 5,378,256 - +0.3 5,378,256 - +0.2 5,378,256 - +1.0 5,378,256 - +1.1 5,378,256 - +0.8 5,378,256 - +0.8 5,378,346 + -0.0 5,378,346 + -1.1 5,378,347 - +2.0 5,378,347 - +1.5 5,378,347 - +0.2 5,378,394 + +0.8 5,378,395 - +0.0 5,378,395 - +1.4 5,378,395 - +0.5 5,378,395 - -0.1 5,378,649 + Pf1N1B4_4863 0.21 -0.8 5,378,649 + Pf1N1B4_4863 0.21 +1.9 5,378,649 + Pf1N1B4_4863 0.21 +0.3 5,378,650 - -0.2 5,378,650 - Pf1N1B4_4863 0.21 +0.8 5,378,650 - Pf1N1B4_4863 0.21 -1.7 5,378,650 - Pf1N1B4_4863 0.21 -0.0 5,378,650 - Pf1N1B4_4863 0.21 +1.1 5,378,650 - Pf1N1B4_4863 0.21 +0.1 5,378,650 - Pf1N1B4_4863 0.21 +1.3 5,378,650 - Pf1N1B4_4863 0.21 -1.0 5,378,732 - Pf1N1B4_4863 0.27 -0.4 5,378,732 - Pf1N1B4_4863 0.27 +0.2 5,378,732 - Pf1N1B4_4863 0.27 +1.5
Or see this region's nucleotide sequence