Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_484

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_482 and Pf1N1B4_483 are separated by 135 nucleotidesPf1N1B4_483 and Pf1N1B4_484 are separated by 92 nucleotidesPf1N1B4_484 and Pf1N1B4_485 are separated by 84 nucleotidesPf1N1B4_485 and Pf1N1B4_486 are separated by 63 nucleotides Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822 _482 Pf1N1B4_483 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 574,958 to 575,539 _483 Pf1N1B4_484 - Hydrogenase maturation factor, at 575,632 to 576,402 _484 Pf1N1B4_485 - L-lysine permease, at 576,487 to 577,041 _485 Pf1N1B4_486 - Tricarboxylate porin OpdH, at 577,105 to 578,346 _486 Position (kb) 575 576 577Strain fitness (log2 ratio) -2 -1 0 1 2at 574.727 kb on + strandat 574.727 kb on + strandat 574.728 kb on - strandat 574.728 kb on - strandat 574.728 kb on - strandat 574.882 kb on - strandat 574.888 kb on - strandat 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.452 kb on - strand, within Pf1N1B4_483at 575.526 kb on - strandat 575.526 kb on - strandat 575.526 kb on - strandat 575.526 kb on - strandat 575.615 kb on + strandat 575.615 kb on + strandat 575.615 kb on + strandat 575.625 kb on + strandat 575.625 kb on + strandat 575.625 kb on + strandat 575.626 kb on - strandat 575.669 kb on + strandat 575.669 kb on + strandat 575.669 kb on + strandat 575.670 kb on - strandat 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.805 kb on + strand, within Pf1N1B4_484at 575.805 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.822 kb on - strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.912 kb on - strand, within Pf1N1B4_484at 575.912 kb on - strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strandat 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.127 kb on + strand, within Pf1N1B4_484at 576.127 kb on + strand, within Pf1N1B4_484at 576.239 kb on + strand, within Pf1N1B4_484at 576.239 kb on + strandat 576.240 kb on - strand, within Pf1N1B4_484at 576.416 kb on + strandat 576.416 kb on + strandat 576.416 kb on + strandat 576.417 kb on - strandat 576.417 kb on - strandat 576.521 kb on + strandat 576.577 kb on + strand, within Pf1N1B4_485at 576.577 kb on + strand, within Pf1N1B4_485at 576.577 kb on + strand, within Pf1N1B4_485at 576.577 kb on + strand, within Pf1N1B4_485at 576.578 kb on - strandat 576.578 kb on - strand, within Pf1N1B4_485at 576.578 kb on - strand, within Pf1N1B4_485at 576.656 kb on - strand, within Pf1N1B4_485at 577.040 kb on - strandat 577.040 kb on - strandat 577.106 kb on + strandat 577.106 kb on + strandat 577.106 kb on + strandat 577.107 kb on - strandat 577.107 kb on - strandat 577.107 kb on - strandat 577.107 kb on - strandat 577.122 kb on + strandat 577.122 kb on + strandat 577.123 kb on - strandat 577.123 kb on - strandat 577.124 kb on + strandat 577.124 kb on + strandat 577.125 kb on - strandat 577.125 kb on - strandat 577.215 kb on + strandat 577.216 kb on - strandat 577.216 kb on - strandat 577.216 kb on - strandat 577.216 kb on - strandat 577.233 kb on - strand, within Pf1N1B4_486at 577.233 kb on - strand, within Pf1N1B4_486at 577.233 kb on - strand, within Pf1N1B4_486at 577.305 kb on + strand, within Pf1N1B4_486at 577.305 kb on + strand, within Pf1N1B4_486at 577.305 kb on + strandat 577.305 kb on + strand, within Pf1N1B4_486at 577.306 kb on - strand, within Pf1N1B4_486at 577.307 kb on + strand, within Pf1N1B4_486at 577.307 kb on + strand, within Pf1N1B4_486at 577.308 kb on - strandat 577.317 kb on + strand, within Pf1N1B4_486at 577.318 kb on - strand, within Pf1N1B4_486at 577.318 kb on - strand, within Pf1N1B4_486at 577.353 kb on + strand, within Pf1N1B4_486at 577.353 kb on + strand, within Pf1N1B4_486at 577.354 kb on - strand, within Pf1N1B4_486at 577.354 kb on - strand, within Pf1N1B4_486at 577.354 kb on - strand, within Pf1N1B4_486at 577.354 kb on - strand, within Pf1N1B4_486at 577.355 kb on + strand, within Pf1N1B4_486at 577.356 kb on - strand, within Pf1N1B4_486at 577.356 kb on - strand, within Pf1N1B4_486at 577.356 kb on - strand, within Pf1N1B4_486

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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574,727 + -0.2
574,727 + -0.9
574,728 - -2.3
574,728 - +0.1
574,728 - -2.1
574,882 - +1.5
574,888 - +0.1
575,451 + Pf1N1B4_483 0.85 -1.2
575,451 + Pf1N1B4_483 0.85 -0.4
575,451 + Pf1N1B4_483 0.85 -1.2
575,451 + Pf1N1B4_483 0.85 -0.6
575,452 - Pf1N1B4_483 0.85 -2.3
575,526 - -0.5
575,526 - +1.1
575,526 - +0.1
575,526 - +0.9
575,615 + +0.1
575,615 + -1.9
575,615 + +0.3
575,625 + -0.5
575,625 + +0.5
575,625 + -1.7
575,626 - +1.5
575,669 + +0.1
575,669 + -0.1
575,669 + +1.3
575,670 - +0.4
575,779 + Pf1N1B4_484 0.19 -1.7
575,779 + Pf1N1B4_484 0.19 -2.5
575,779 + Pf1N1B4_484 0.19 +0.5
575,779 + Pf1N1B4_484 0.19 -1.9
575,779 + Pf1N1B4_484 0.19 -0.2
575,780 - Pf1N1B4_484 0.19 +2.4
575,780 - Pf1N1B4_484 0.19 +1.4
575,780 - Pf1N1B4_484 0.19 +0.1
575,805 + Pf1N1B4_484 0.22 +1.4
575,805 + Pf1N1B4_484 0.22 +0.4
575,821 + Pf1N1B4_484 0.25 +1.4
575,821 + Pf1N1B4_484 0.25 +0.1
575,821 + Pf1N1B4_484 0.25 -2.5
575,822 - Pf1N1B4_484 0.25 -0.5
575,857 + Pf1N1B4_484 0.29 +1.6
575,857 + Pf1N1B4_484 0.29 +1.4
575,857 + Pf1N1B4_484 0.29 +0.3
575,912 - Pf1N1B4_484 0.36 -1.9
575,912 - Pf1N1B4_484 0.36 +0.7
576,001 + Pf1N1B4_484 0.48 -0.5
576,001 + Pf1N1B4_484 0.48 -0.9
576,001 + Pf1N1B4_484 0.48 +1.1
576,001 + Pf1N1B4_484 0.48 +0.3
576,001 + Pf1N1B4_484 0.48 -0.9
576,001 + Pf1N1B4_484 0.48 +1.8
576,001 + -0.5
576,001 + Pf1N1B4_484 0.48 -0.8
576,001 + Pf1N1B4_484 0.48 -2.0
576,001 + Pf1N1B4_484 0.48 -0.8
576,001 + Pf1N1B4_484 0.48 +0.9
576,001 + Pf1N1B4_484 0.48 +1.3
576,001 + Pf1N1B4_484 0.48 -0.9
576,001 + Pf1N1B4_484 0.48 -2.0
576,001 + Pf1N1B4_484 0.48 -0.2
576,001 + Pf1N1B4_484 0.48 +1.1
576,001 + Pf1N1B4_484 0.48 -1.2
576,001 + Pf1N1B4_484 0.48 -1.9
576,001 + Pf1N1B4_484 0.48 +0.6
576,001 + Pf1N1B4_484 0.48 -1.0
576,001 + Pf1N1B4_484 0.48 -2.1
576,001 + Pf1N1B4_484 0.48 -0.2
576,001 + Pf1N1B4_484 0.48 -1.2
576,001 + Pf1N1B4_484 0.48 +1.8
576,002 - Pf1N1B4_484 0.48 +1.1
576,002 - Pf1N1B4_484 0.48 +0.6
576,002 - Pf1N1B4_484 0.48 -0.5
576,002 - Pf1N1B4_484 0.48 +0.6
576,002 - Pf1N1B4_484 0.48 -1.2
576,002 - Pf1N1B4_484 0.48 -0.9
576,002 - Pf1N1B4_484 0.48 -1.2
576,002 - Pf1N1B4_484 0.48 +0.6
576,002 - Pf1N1B4_484 0.48 +0.5
576,002 - Pf1N1B4_484 0.48 -0.3
576,002 - Pf1N1B4_484 0.48 -1.5
576,002 - Pf1N1B4_484 0.48 +1.1
576,002 - Pf1N1B4_484 0.48 +1.9
576,002 - Pf1N1B4_484 0.48 +0.4
576,127 + Pf1N1B4_484 0.64 -0.3
576,127 + Pf1N1B4_484 0.64 +1.5
576,239 + Pf1N1B4_484 0.79 -1.3
576,239 + +1.9
576,240 - Pf1N1B4_484 0.79 +0.2
576,416 + +0.8
576,416 + -0.9
576,416 + +0.5
576,417 - +0.5
576,417 - -0.5
576,521 + -2.7
576,577 + Pf1N1B4_485 0.16 +0.7
576,577 + Pf1N1B4_485 0.16 -0.9
576,577 + Pf1N1B4_485 0.16 -2.2
576,577 + Pf1N1B4_485 0.16 +1.5
576,578 - -0.5
576,578 - Pf1N1B4_485 0.16 -0.8
576,578 - Pf1N1B4_485 0.16 -0.3
576,656 - Pf1N1B4_485 0.30 +2.6
577,040 - -1.5
577,040 - +0.9
577,106 + -1.9
577,106 + +0.1
577,106 + -1.0
577,107 - +1.2
577,107 - +1.1
577,107 - +0.8
577,107 - +1.1
577,122 + +1.1
577,122 + -0.5
577,123 - +0.3
577,123 - -1.2
577,124 + -1.7
577,124 + +0.9
577,125 - +0.0
577,125 - +1.9
577,215 + -1.2
577,216 - -0.5
577,216 - +0.8
577,216 - +0.6
577,216 - +1.4
577,233 - Pf1N1B4_486 0.10 +0.3
577,233 - Pf1N1B4_486 0.10 +0.9
577,233 - Pf1N1B4_486 0.10 +0.3
577,305 + Pf1N1B4_486 0.16 -1.5
577,305 + Pf1N1B4_486 0.16 +0.5
577,305 + -0.5
577,305 + Pf1N1B4_486 0.16 +1.1
577,306 - Pf1N1B4_486 0.16 -0.3
577,307 + Pf1N1B4_486 0.16 +0.2
577,307 + Pf1N1B4_486 0.16 -1.2
577,308 - +0.1
577,317 + Pf1N1B4_486 0.17 -0.3
577,318 - Pf1N1B4_486 0.17 -1.2
577,318 - Pf1N1B4_486 0.17 -1.7
577,353 + Pf1N1B4_486 0.20 +0.5
577,353 + Pf1N1B4_486 0.20 +0.7
577,354 - Pf1N1B4_486 0.20 -1.9
577,354 - Pf1N1B4_486 0.20 +0.8
577,354 - Pf1N1B4_486 0.20 +0.9
577,354 - Pf1N1B4_486 0.20 -1.2
577,355 + Pf1N1B4_486 0.20 -0.7
577,356 - Pf1N1B4_486 0.20 +0.9
577,356 - Pf1N1B4_486 0.20 -0.7
577,356 - Pf1N1B4_486 0.20 -1.2

Or see this region's nucleotide sequence