Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4810

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4809 and Pf1N1B4_4810 overlap by 4 nucleotidesPf1N1B4_4810 and Pf1N1B4_4811 overlap by 4 nucleotidesPf1N1B4_4811 and Pf1N1B4_4812 are separated by 575 nucleotides Pf1N1B4_4809 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 5,312,015 to 5,313,394 _4809 Pf1N1B4_4810 - Polymyxin resistance protein PmrM, at 5,313,391 to 5,313,804 _4810 Pf1N1B4_4811 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 5,313,801 to 5,314,145 _4811 Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692 _4812 Position (kb) 5313 5314Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5312.466 kb on + strand, within Pf1N1B4_4809at 5312.466 kb on + strand, within Pf1N1B4_4809at 5313.367 kb on - strandat 5313.381 kb on - strandat 5313.530 kb on + strandat 5313.530 kb on + strand, within Pf1N1B4_4810at 5313.543 kb on - strand, within Pf1N1B4_4810at 5313.563 kb on + strand, within Pf1N1B4_4810at 5313.663 kb on + strand, within Pf1N1B4_4810at 5314.129 kb on - strandat 5314.356 kb on - strandat 5314.379 kb on + strandat 5314.379 kb on + strandat 5314.380 kb on - strandat 5314.380 kb on - strandat 5314.663 kb on - strandat 5314.691 kb on - strandat 5314.693 kb on - strandat 5314.700 kb on - strandat 5314.765 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,312,466 + Pf1N1B4_4809 0.33 -1.6
5,312,466 + Pf1N1B4_4809 0.33 +1.0
5,313,367 - -0.2
5,313,381 - +1.6
5,313,530 + +2.8
5,313,530 + Pf1N1B4_4810 0.34 -1.6
5,313,543 - Pf1N1B4_4810 0.37 -2.2
5,313,563 + Pf1N1B4_4810 0.42 -0.2
5,313,663 + Pf1N1B4_4810 0.66 -1.4
5,314,129 - -0.0
5,314,356 - +0.8
5,314,379 + -0.2
5,314,379 + -0.1
5,314,380 - +2.1
5,314,380 - -0.2
5,314,663 - +0.0
5,314,691 - +0.4
5,314,693 - +1.0
5,314,700 - -0.1
5,314,765 - -1.6

Or see this region's nucleotide sequence