Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_480 and Pf1N1B4_481 are separated by 100 nucleotides Pf1N1B4_481 and Pf1N1B4_482 are separated by 196 nucleotides
Pf1N1B4_480 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 570,930 to 573,119
_480
Pf1N1B4_481 - FIG00956241: hypothetical protein, at 573,220 to 573,627
_481
Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822
_482
Position (kb) 573
574 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 572.416 kb on - strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.533 kb on + strand, within Pf1N1B4_480 at 572.534 kb on - strand, within Pf1N1B4_480 at 572.534 kb on - strand, within Pf1N1B4_480 at 572.534 kb on - strand, within Pf1N1B4_480 at 572.542 kb on - strand, within Pf1N1B4_480 at 572.547 kb on + strand, within Pf1N1B4_480 at 572.548 kb on - strand, within Pf1N1B4_480 at 572.548 kb on - strand, within Pf1N1B4_480 at 572.600 kb on + strand, within Pf1N1B4_480 at 572.600 kb on + strand, within Pf1N1B4_480 at 572.692 kb on + strand, within Pf1N1B4_480 at 572.693 kb on - strand, within Pf1N1B4_480 at 572.693 kb on - strand, within Pf1N1B4_480 at 572.693 kb on - strand, within Pf1N1B4_480 at 572.822 kb on - strand, within Pf1N1B4_480 at 573.095 kb on - strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.154 kb on - strand at 573.154 kb on - strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.182 kb on - strand at 573.182 kb on - strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.284 kb on + strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.386 kb on - strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.491 kb on - strand at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.517 kb on + strand, within Pf1N1B4_481 at 573.593 kb on + strand at 573.662 kb on + strand at 573.663 kb on - strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.856 kb on - strand at 573.856 kb on - strand at 573.937 kb on - strand, within Pf1N1B4_482 at 574.083 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 572,416 - Pf1N1B4_480 0.68 +0.8 572,533 + Pf1N1B4_480 0.73 +0.7 572,533 + Pf1N1B4_480 0.73 +0.1 572,533 + Pf1N1B4_480 0.73 +1.9 572,533 + Pf1N1B4_480 0.73 -0.1 572,533 + Pf1N1B4_480 0.73 -0.9 572,533 + Pf1N1B4_480 0.73 +0.5 572,533 + Pf1N1B4_480 0.73 -0.5 572,534 - Pf1N1B4_480 0.73 +0.3 572,534 - Pf1N1B4_480 0.73 +0.0 572,534 - Pf1N1B4_480 0.73 -1.9 572,542 - Pf1N1B4_480 0.74 -1.3 572,547 + Pf1N1B4_480 0.74 +0.1 572,548 - Pf1N1B4_480 0.74 +1.3 572,548 - Pf1N1B4_480 0.74 -0.6 572,600 + Pf1N1B4_480 0.76 -0.8 572,600 + Pf1N1B4_480 0.76 -1.5 572,692 + Pf1N1B4_480 0.80 -0.9 572,693 - Pf1N1B4_480 0.81 +1.5 572,693 - Pf1N1B4_480 0.81 -1.2 572,693 - Pf1N1B4_480 0.81 +1.7 572,822 - Pf1N1B4_480 0.86 -0.1 573,095 - -0.5 573,153 + +0.3 573,153 + +0.7 573,153 + -0.5 573,154 - -0.4 573,154 - -0.8 573,156 + +0.7 573,156 + -0.9 573,156 + +0.9 573,156 + +3.1 573,156 + -0.5 573,156 + +0.5 573,157 - +0.2 573,157 - -0.2 573,157 - -2.6 573,157 - -1.2 573,157 - +0.9 573,157 - +1.8 573,157 - +0.6 573,181 + +0.1 573,181 + -0.9 573,181 + -0.9 573,182 - +1.3 573,182 - -0.3 573,183 + +0.3 573,183 + +1.7 573,183 + +3.6 573,184 - -1.7 573,184 - -1.2 573,184 - -1.2 573,184 - -1.7 573,184 - +0.8 573,214 + -0.2 573,214 + -0.9 573,214 + +3.1 573,214 + -2.5 573,215 - -0.3 573,215 - -1.5 573,215 - +1.8 573,215 - +0.6 573,284 + Pf1N1B4_481 0.16 +1.7 573,285 - Pf1N1B4_481 0.16 +1.1 573,285 - Pf1N1B4_481 0.16 +0.1 573,285 - Pf1N1B4_481 0.16 +0.6 573,386 - Pf1N1B4_481 0.41 +0.4 573,466 + Pf1N1B4_481 0.60 -1.7 573,466 + Pf1N1B4_481 0.60 +2.3 573,466 + +0.1 573,490 + Pf1N1B4_481 0.66 -2.7 573,490 + Pf1N1B4_481 0.66 -1.2 573,490 + Pf1N1B4_481 0.66 -0.9 573,490 + Pf1N1B4_481 0.66 -1.9 573,490 + Pf1N1B4_481 0.66 -0.7 573,490 + Pf1N1B4_481 0.66 +0.9 573,490 + Pf1N1B4_481 0.66 -1.2 573,490 + Pf1N1B4_481 0.66 -0.9 573,491 - -1.2 573,491 - Pf1N1B4_481 0.66 -1.1 573,491 - Pf1N1B4_481 0.66 +1.8 573,491 - Pf1N1B4_481 0.66 -1.2 573,491 - Pf1N1B4_481 0.66 -1.1 573,491 - Pf1N1B4_481 0.66 -1.1 573,491 - Pf1N1B4_481 0.66 -0.1 573,517 + Pf1N1B4_481 0.73 +0.7 573,593 + -1.2 573,662 + +1.0 573,663 - -0.1 573,855 + -1.9 573,855 + -0.5 573,855 + -2.2 573,856 - -1.5 573,856 - +0.7 573,937 - Pf1N1B4_482 0.11 -2.2 574,083 + Pf1N1B4_482 0.26 -0.9 574,233 + Pf1N1B4_482 0.41 +0.1 574,233 + Pf1N1B4_482 0.41 +0.9 574,234 - Pf1N1B4_482 0.41 -1.7 574,234 - Pf1N1B4_482 0.41 -1.2 574,237 + Pf1N1B4_482 0.41 +1.7 574,237 + Pf1N1B4_482 0.41 -0.1 574,238 - Pf1N1B4_482 0.41 +0.1 574,238 - Pf1N1B4_482 0.41 -0.3 574,238 - Pf1N1B4_482 0.41 +0.8 574,238 - Pf1N1B4_482 0.41 -1.5 574,238 - Pf1N1B4_482 0.41 +1.1 574,238 - Pf1N1B4_482 0.41 -2.1 574,238 - Pf1N1B4_482 0.41 +1.8 574,238 - Pf1N1B4_482 0.41 +0.5 574,313 + Pf1N1B4_482 0.49 -0.0 574,313 + Pf1N1B4_482 0.49 -1.3 574,314 - Pf1N1B4_482 0.49 +0.3 574,314 - Pf1N1B4_482 0.49 +2.4 574,314 - Pf1N1B4_482 0.49 -0.2
Or see this region's nucleotide sequence