Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4751

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4750 and Pf1N1B4_4751 are separated by 161 nucleotidesPf1N1B4_4751 and Pf1N1B4_4752 are separated by 222 nucleotidesPf1N1B4_4752 and Pf1N1B4_4753 are separated by 51 nucleotides Pf1N1B4_4750 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 5,247,959 to 5,248,795 _4750 Pf1N1B4_4751 - Ku domain protein, at 5,248,957 to 5,249,817 _4751 Pf1N1B4_4752 - FIG00962948: hypothetical protein, at 5,250,040 to 5,250,384 _4752 Pf1N1B4_4753 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 5,250,436 to 5,251,587 _4753 Position (kb) 5248 5249 5250Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5247.973 kb on - strandat 5247.985 kb on - strandat 5248.028 kb on + strandat 5248.029 kb on - strandat 5248.029 kb on - strandat 5248.059 kb on - strand, within Pf1N1B4_4750at 5248.173 kb on - strand, within Pf1N1B4_4750at 5248.382 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strandat 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.427 kb on + strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strandat 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.428 kb on - strand, within Pf1N1B4_4750at 5248.430 kb on - strand, within Pf1N1B4_4750at 5248.451 kb on + strand, within Pf1N1B4_4750at 5248.451 kb on + strand, within Pf1N1B4_4750at 5248.451 kb on + strand, within Pf1N1B4_4750at 5248.452 kb on - strand, within Pf1N1B4_4750at 5248.502 kb on + strand, within Pf1N1B4_4750at 5248.503 kb on - strand, within Pf1N1B4_4750at 5248.568 kb on + strand, within Pf1N1B4_4750at 5248.569 kb on - strandat 5248.660 kb on + strand, within Pf1N1B4_4750at 5248.660 kb on + strand, within Pf1N1B4_4750at 5248.661 kb on - strand, within Pf1N1B4_4750at 5248.661 kb on - strand, within Pf1N1B4_4750at 5248.685 kb on + strand, within Pf1N1B4_4750at 5248.686 kb on - strand, within Pf1N1B4_4750at 5248.686 kb on - strand, within Pf1N1B4_4750at 5248.724 kb on - strandat 5248.737 kb on - strandat 5248.739 kb on + strandat 5248.739 kb on + strandat 5248.740 kb on - strandat 5248.860 kb on - strandat 5248.860 kb on - strandat 5248.919 kb on - strandat 5248.936 kb on + strandat 5248.936 kb on + strandat 5248.946 kb on - strandat 5248.946 kb on - strandat 5248.946 kb on - strandat 5249.071 kb on - strand, within Pf1N1B4_4751at 5249.086 kb on - strandat 5249.450 kb on + strandat 5249.451 kb on - strand, within Pf1N1B4_4751at 5249.451 kb on - strandat 5249.483 kb on + strand, within Pf1N1B4_4751at 5249.484 kb on - strand, within Pf1N1B4_4751at 5249.484 kb on - strand, within Pf1N1B4_4751at 5249.487 kb on - strand, within Pf1N1B4_4751at 5249.487 kb on - strand, within Pf1N1B4_4751at 5249.603 kb on + strand, within Pf1N1B4_4751at 5249.604 kb on - strandat 5249.619 kb on - strand, within Pf1N1B4_4751at 5249.841 kb on - strandat 5249.841 kb on - strandat 5249.841 kb on - strandat 5249.927 kb on - strandat 5249.948 kb on - strandat 5249.951 kb on + strandat 5250.015 kb on - strandat 5250.376 kb on + strandat 5250.377 kb on - strandat 5250.413 kb on - strandat 5250.444 kb on + strandat 5250.526 kb on - strandat 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.773 kb on - strand, within Pf1N1B4_4753at 5250.773 kb on - strand, within Pf1N1B4_4753at 5250.773 kb on - strandat 5250.773 kb on - strand, within Pf1N1B4_4753

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,247,973 - +0.2
5,247,985 - -2.2
5,248,028 + -0.6
5,248,029 - -0.2
5,248,029 - +0.2
5,248,059 - Pf1N1B4_4750 0.12 +1.1
5,248,173 - Pf1N1B4_4750 0.26 -2.5
5,248,382 + Pf1N1B4_4750 0.51 -0.6
5,248,427 + Pf1N1B4_4750 0.56 -0.6
5,248,427 + Pf1N1B4_4750 0.56 -3.2
5,248,427 + Pf1N1B4_4750 0.56 -0.4
5,248,427 + Pf1N1B4_4750 0.56 -0.1
5,248,427 + +0.4
5,248,427 + Pf1N1B4_4750 0.56 -1.1
5,248,427 + Pf1N1B4_4750 0.56 -1.2
5,248,427 + Pf1N1B4_4750 0.56 +0.4
5,248,427 + Pf1N1B4_4750 0.56 -1.2
5,248,427 + Pf1N1B4_4750 0.56 -2.9
5,248,427 + Pf1N1B4_4750 0.56 -0.2
5,248,428 - Pf1N1B4_4750 0.56 +0.2
5,248,428 - Pf1N1B4_4750 0.56 -2.7
5,248,428 - Pf1N1B4_4750 0.56 -1.6
5,248,428 - Pf1N1B4_4750 0.56 -1.7
5,248,428 - -1.8
5,248,428 - Pf1N1B4_4750 0.56 -2.0
5,248,428 - Pf1N1B4_4750 0.56 +0.8
5,248,428 - Pf1N1B4_4750 0.56 -0.8
5,248,428 - Pf1N1B4_4750 0.56 -0.8
5,248,428 - Pf1N1B4_4750 0.56 -1.6
5,248,430 - Pf1N1B4_4750 0.56 -1.9
5,248,451 + Pf1N1B4_4750 0.59 +0.8
5,248,451 + Pf1N1B4_4750 0.59 -1.0
5,248,451 + Pf1N1B4_4750 0.59 -0.8
5,248,452 - Pf1N1B4_4750 0.59 -1.2
5,248,502 + Pf1N1B4_4750 0.65 +0.3
5,248,503 - Pf1N1B4_4750 0.65 -0.4
5,248,568 + Pf1N1B4_4750 0.73 -1.2
5,248,569 - -0.2
5,248,660 + Pf1N1B4_4750 0.84 -1.4
5,248,660 + Pf1N1B4_4750 0.84 -1.5
5,248,661 - Pf1N1B4_4750 0.84 -2.2
5,248,661 - Pf1N1B4_4750 0.84 +0.8
5,248,685 + Pf1N1B4_4750 0.87 -1.4
5,248,686 - Pf1N1B4_4750 0.87 -0.8
5,248,686 - Pf1N1B4_4750 0.87 -1.2
5,248,724 - -1.8
5,248,737 - -2.6
5,248,739 + -2.2
5,248,739 + -1.6
5,248,740 - -2.4
5,248,860 - +1.2
5,248,860 - -1.8
5,248,919 - -1.2
5,248,936 + +0.2
5,248,936 + -0.2
5,248,946 - +0.3
5,248,946 - -2.9
5,248,946 - -1.0
5,249,071 - Pf1N1B4_4751 0.13 +0.8
5,249,086 - +1.8
5,249,450 + +0.8
5,249,451 - Pf1N1B4_4751 0.57 +0.2
5,249,451 - +1.6
5,249,483 + Pf1N1B4_4751 0.61 -2.2
5,249,484 - Pf1N1B4_4751 0.61 +0.8
5,249,484 - Pf1N1B4_4751 0.61 +0.2
5,249,487 - Pf1N1B4_4751 0.62 +0.0
5,249,487 - Pf1N1B4_4751 0.62 +0.3
5,249,603 + Pf1N1B4_4751 0.75 -2.0
5,249,604 - -0.2
5,249,619 - Pf1N1B4_4751 0.77 -0.8
5,249,841 - +1.0
5,249,841 - +2.1
5,249,841 - -1.6
5,249,927 - -0.0
5,249,948 - -0.2
5,249,951 + -0.2
5,250,015 - -1.6
5,250,376 + +0.2
5,250,377 - +0.0
5,250,413 - +0.4
5,250,444 + +0.8
5,250,526 - +0.5
5,250,772 + Pf1N1B4_4753 0.29 +0.2
5,250,772 + Pf1N1B4_4753 0.29 -1.2
5,250,772 + Pf1N1B4_4753 0.29 -0.2
5,250,772 + Pf1N1B4_4753 0.29 +0.2
5,250,772 + Pf1N1B4_4753 0.29 -0.5
5,250,772 + Pf1N1B4_4753 0.29 -1.6
5,250,773 - Pf1N1B4_4753 0.29 +0.5
5,250,773 - Pf1N1B4_4753 0.29 -0.8
5,250,773 - +1.3
5,250,773 - Pf1N1B4_4753 0.29 +0.2

Or see this region's nucleotide sequence