Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4723

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4721 and Pf1N1B4_4722 are separated by 116 nucleotidesPf1N1B4_4722 and Pf1N1B4_4723 are separated by 194 nucleotidesPf1N1B4_4723 and Pf1N1B4_4724 are separated by 198 nucleotidesPf1N1B4_4724 and Pf1N1B4_4725 are separated by 272 nucleotides Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979 _4721 Pf1N1B4_4722 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,218,096 to 5,218,587 _4722 Pf1N1B4_4723 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 5,218,782 to 5,220,527 _4723 Pf1N1B4_4724 - FIG00961385: hypothetical protein, at 5,220,726 to 5,220,908 _4724 Pf1N1B4_4725 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 5,221,181 to 5,222,161 _4725 Position (kb) 5218 5219 5220 5221Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5218.132 kb on + strandat 5218.169 kb on + strandat 5218.224 kb on + strand, within Pf1N1B4_4722at 5218.489 kb on - strand, within Pf1N1B4_4722at 5218.698 kb on + strandat 5218.751 kb on + strandat 5218.751 kb on + strandat 5218.783 kb on + strandat 5218.809 kb on - strandat 5218.868 kb on - strandat 5219.164 kb on + strand, within Pf1N1B4_4723at 5219.165 kb on - strandat 5219.233 kb on + strand, within Pf1N1B4_4723at 5219.348 kb on - strandat 5219.761 kb on + strand, within Pf1N1B4_4723at 5220.085 kb on + strand, within Pf1N1B4_4723at 5220.178 kb on + strand, within Pf1N1B4_4723at 5220.178 kb on + strandat 5220.405 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.637 kb on - strandat 5220.637 kb on - strandat 5220.637 kb on - strandat 5220.898 kb on - strandat 5220.940 kb on - strandat 5220.970 kb on - strandat 5221.137 kb on + strandat 5221.137 kb on + strandat 5221.137 kb on + strandat 5221.157 kb on - strandat 5221.162 kb on + strandat 5221.166 kb on - strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.179 kb on - strandat 5221.179 kb on - strandat 5221.222 kb on + strandat 5221.252 kb on + strandat 5221.253 kb on - strandat 5221.265 kb on + strandat 5221.423 kb on + strand, within Pf1N1B4_4725at 5221.423 kb on + strand, within Pf1N1B4_4725at 5221.424 kb on - strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.473 kb on - strandat 5221.473 kb on - strand, within Pf1N1B4_4725at 5221.475 kb on - strand, within Pf1N1B4_4725

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,218,132 + +2.1
5,218,169 + -0.7
5,218,224 + Pf1N1B4_4722 0.26 +1.5
5,218,489 - Pf1N1B4_4722 0.80 -0.2
5,218,698 + +0.7
5,218,751 + +1.4
5,218,751 + +0.7
5,218,783 + -0.1
5,218,809 - +1.5
5,218,868 - +0.9
5,219,164 + Pf1N1B4_4723 0.22 +2.2
5,219,165 - -0.1
5,219,233 + Pf1N1B4_4723 0.26 +2.2
5,219,348 - -0.1
5,219,761 + Pf1N1B4_4723 0.56 +0.6
5,220,085 + Pf1N1B4_4723 0.75 +0.9
5,220,178 + Pf1N1B4_4723 0.80 +0.3
5,220,178 + +1.2
5,220,405 - +0.6
5,220,550 - -1.1
5,220,550 - -2.3
5,220,550 - -2.3
5,220,550 - -2.8
5,220,550 - -4.6
5,220,550 - -2.5
5,220,637 - -2.0
5,220,637 - -3.5
5,220,637 - -2.5
5,220,898 - +2.2
5,220,940 - -0.3
5,220,970 - -0.7
5,221,137 + +2.6
5,221,137 + -0.7
5,221,137 + -0.2
5,221,157 - -0.7
5,221,162 + +1.1
5,221,166 - -0.3
5,221,178 + -1.2
5,221,178 + +1.4
5,221,178 + +1.0
5,221,178 + +1.0
5,221,179 - -2.3
5,221,179 - -0.2
5,221,222 + +2.4
5,221,252 + +0.1
5,221,253 - -0.4
5,221,265 + +0.5
5,221,423 + Pf1N1B4_4725 0.25 +0.8
5,221,423 + Pf1N1B4_4725 0.25 +1.3
5,221,424 - Pf1N1B4_4725 0.25 +0.4
5,221,472 + Pf1N1B4_4725 0.30 +1.7
5,221,472 + Pf1N1B4_4725 0.30 +1.1
5,221,472 + Pf1N1B4_4725 0.30 -0.7
5,221,473 - +1.4
5,221,473 - Pf1N1B4_4725 0.30 +0.4
5,221,475 - Pf1N1B4_4725 0.30 +1.4

Or see this region's nucleotide sequence