Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotides Pf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides Pf1N1B4_4720 and Pf1N1B4_4721 are separated by 18 nucleotides Pf1N1B4_4721 and Pf1N1B4_4722 are separated by 116 nucleotides
Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720
_4718
Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114
_4719
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979
_4721
Pf1N1B4_4722 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,218,096 to 5,218,587
_4722
Position (kb) 5216
5217
5218 Strain fitness (log2 ratio) -1
0
1
2
3 at 5216.058 kb on + strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.245 kb on - strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.249 kb on - strand at 5216.249 kb on - strand at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand at 5216.725 kb on - strand, within Pf1N1B4_4720 at 5217.261 kb on - strand, within Pf1N1B4_4720 at 5217.294 kb on - strand at 5217.306 kb on - strand at 5217.576 kb on - strand at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.703 kb on + strand, within Pf1N1B4_4721 at 5218.132 kb on + strand at 5218.169 kb on + strand at 5218.224 kb on + strand, within Pf1N1B4_4722
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,216,058 + Pf1N1B4_4719 0.83 -0.2 5,216,059 - Pf1N1B4_4719 0.84 -1.2 5,216,059 - Pf1N1B4_4719 0.84 +0.3 5,216,245 - +1.4 5,216,248 + -0.3 5,216,248 + +0.5 5,216,248 + -1.7 5,216,248 + -0.6 5,216,249 - -1.5 5,216,249 - +3.7 5,216,409 - Pf1N1B4_4720 0.13 +1.4 5,216,409 - Pf1N1B4_4720 0.13 +1.7 5,216,409 - -0.7 5,216,725 - Pf1N1B4_4720 0.41 -0.4 5,217,261 - Pf1N1B4_4720 0.89 -1.5 5,217,294 - +0.3 5,217,306 - -0.2 5,217,576 - +0.4 5,217,576 - Pf1N1B4_4721 0.31 -0.4 5,217,576 - Pf1N1B4_4721 0.31 -0.2 5,217,703 + Pf1N1B4_4721 0.52 +1.6 5,218,132 + +2.1 5,218,169 + -0.7 5,218,224 + Pf1N1B4_4722 0.26 +1.5
Or see this region's nucleotide sequence