Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4606 and Pf1N1B4_4607 are separated by 22 nucleotides Pf1N1B4_4607 and Pf1N1B4_4608 overlap by 25 nucleotides Pf1N1B4_4608 and Pf1N1B4_4609 overlap by 1 nucleotides Pf1N1B4_4609 and Pf1N1B4_4610 overlap by 31 nucleotides Pf1N1B4_4610 and Pf1N1B4_4611 overlap by 4 nucleotides
Pf1N1B4_4606 - NAD kinase (EC 2.7.1.23), at 5,079,044 to 5,079,364
_4606
Pf1N1B4_4607 - NAD kinase (EC 2.7.1.23), at 5,079,387 to 5,079,728
_4607
Pf1N1B4_4608 - NAD kinase (EC 2.7.1.23), at 5,079,704 to 5,079,934
_4608
Pf1N1B4_4609 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,079,934 to 5,080,398
_4609
Pf1N1B4_4610 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,080,368 to 5,080,904
_4610
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Position (kb)
5079
5080
5081 Strain fitness (log2 ratio)
-2
-1
0
1 at 5079.951 kb on + strand at 5079.956 kb on - strand at 5080.243 kb on - strand, within Pf1N1B4_4609 at 5080.329 kb on + strand, within Pf1N1B4_4609 at 5080.701 kb on + strand, within Pf1N1B4_4610 at 5080.737 kb on + strand, within Pf1N1B4_4610 at 5080.859 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.921 kb on - strand at 5081.019 kb on + strand, within Pf1N1B4_4611 at 5081.136 kb on + strand, within Pf1N1B4_4611
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,079,951 + -0.7 5,079,956 - -2.4 5,080,243 - Pf1N1B4_4609 0.66 +0.5 5,080,329 + Pf1N1B4_4609 0.85 -1.7 5,080,701 + Pf1N1B4_4610 0.62 -2.0 5,080,737 + Pf1N1B4_4610 0.69 +0.8 5,080,859 + -0.5 5,080,920 + -1.5 5,080,920 + +0.1 5,080,920 + -0.3 5,080,920 + +1.2 5,080,921 - -0.2 5,081,019 + Pf1N1B4_4611 0.14 +1.7 5,081,136 + Pf1N1B4_4611 0.27 -2.2
Or see this region's nucleotide sequence