Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4604 and Pf1N1B4_4605 are separated by 229 nucleotides Pf1N1B4_4605 and Pf1N1B4_4606 are separated by 125 nucleotides Pf1N1B4_4606 and Pf1N1B4_4607 are separated by 22 nucleotides Pf1N1B4_4607 and Pf1N1B4_4608 overlap by 25 nucleotides
Pf1N1B4_4604 - FMN reductase, NADPH-dependent, at 5,077,178 to 5,077,738
_4604
Pf1N1B4_4605 - FIG00953564: hypothetical protein, at 5,077,968 to 5,078,918
_4605
Pf1N1B4_4606 - NAD kinase (EC 2.7.1.23), at 5,079,044 to 5,079,364
_4606
Pf1N1B4_4607 - NAD kinase (EC 2.7.1.23), at 5,079,387 to 5,079,728
_4607
Pf1N1B4_4608 - NAD kinase (EC 2.7.1.23), at 5,079,704 to 5,079,934
_4608
Position (kb)
5077
5078
5079 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5077.038 kb on + strand at 5077.058 kb on + strand at 5077.136 kb on + strand at 5077.136 kb on + strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.139 kb on - strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.143 kb on - strand at 5077.190 kb on + strand at 5077.320 kb on - strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.616 kb on + strand, within Pf1N1B4_4604 at 5077.617 kb on - strand, within Pf1N1B4_4604 at 5077.726 kb on + strand at 5077.726 kb on + strand at 5077.726 kb on + strand at 5077.727 kb on - strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.745 kb on + strand at 5077.746 kb on - strand at 5077.746 kb on - strand at 5077.759 kb on + strand at 5077.759 kb on + strand at 5077.759 kb on + strand at 5077.760 kb on - strand at 5077.861 kb on - strand at 5078.315 kb on - strand at 5078.665 kb on + strand, within Pf1N1B4_4605 at 5078.665 kb on + strand at 5078.665 kb on + strand, within Pf1N1B4_4605 at 5078.668 kb on + strand, within Pf1N1B4_4605 at 5078.719 kb on + strand, within Pf1N1B4_4605 at 5078.720 kb on - strand, within Pf1N1B4_4605 at 5078.890 kb on - strand at 5078.910 kb on + strand at 5078.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,077,038 + -2.2 5,077,058 + -0.2 5,077,136 + +1.0 5,077,136 + -0.6 5,077,137 - -1.2 5,077,137 - -0.8 5,077,137 - +0.8 5,077,137 - -2.6 5,077,138 + +0.6 5,077,138 + -2.0 5,077,138 + -0.8 5,077,139 - -2.2 5,077,142 + +0.8 5,077,142 + -0.1 5,077,142 + +0.6 5,077,142 + -1.0 5,077,142 + -0.3 5,077,143 - -2.0 5,077,190 + +0.2 5,077,320 - Pf1N1B4_4604 0.25 +1.6 5,077,418 + Pf1N1B4_4604 0.43 -1.0 5,077,418 + Pf1N1B4_4604 0.43 +0.8 5,077,616 + Pf1N1B4_4604 0.78 -1.8 5,077,617 - Pf1N1B4_4604 0.78 +1.2 5,077,726 + +1.4 5,077,726 + -2.6 5,077,726 + +2.8 5,077,727 - -0.8 5,077,745 + -1.2 5,077,745 + -0.3 5,077,745 + -0.1 5,077,745 + +2.2 5,077,745 + -1.0 5,077,745 + +2.1 5,077,745 + +0.8 5,077,746 - +0.4 5,077,746 - -0.2 5,077,759 + -1.2 5,077,759 + -2.6 5,077,759 + +1.2 5,077,760 - -2.2 5,077,861 - -1.2 5,078,315 - +2.4 5,078,665 + Pf1N1B4_4605 0.73 -0.1 5,078,665 + +0.2 5,078,665 + Pf1N1B4_4605 0.73 +0.2 5,078,668 + Pf1N1B4_4605 0.74 +0.7 5,078,719 + Pf1N1B4_4605 0.79 +0.3 5,078,720 - Pf1N1B4_4605 0.79 +1.1 5,078,890 - +1.5 5,078,910 + -0.5 5,078,911 - +0.2
Or see this region's nucleotide sequence