Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4375

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4373 and Pf1N1B4_4374 are separated by 434 nucleotidesPf1N1B4_4374 and Pf1N1B4_4375 are separated by 14 nucleotidesPf1N1B4_4375 and Pf1N1B4_4376 are separated by 36 nucleotides Pf1N1B4_4373 - hypothetical protein, at 4,830,760 to 4,830,903 _4373 Pf1N1B4_4374 - hypothetical protein, at 4,831,338 to 4,831,736 _4374 Pf1N1B4_4375 - Chromate transport protein ChrA, at 4,831,751 to 4,833,109 _4375 Pf1N1B4_4376 - probable transmembrane protein, at 4,833,146 to 4,833,997 _4376 Position (kb) 4831 4832 4833 4834Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4830.775 kb on + strandat 4830.775 kb on + strand, within Pf1N1B4_4373at 4830.775 kb on + strand, within Pf1N1B4_4373at 4830.901 kb on + strandat 4830.902 kb on - strandat 4831.020 kb on + strandat 4831.020 kb on + strandat 4831.070 kb on + strandat 4831.070 kb on + strandat 4831.071 kb on - strandat 4831.119 kb on + strandat 4831.269 kb on + strandat 4831.269 kb on + strandat 4831.269 kb on + strandat 4831.270 kb on - strandat 4831.569 kb on + strand, within Pf1N1B4_4374at 4831.570 kb on - strand, within Pf1N1B4_4374at 4831.679 kb on - strand, within Pf1N1B4_4374at 4831.679 kb on - strand, within Pf1N1B4_4374at 4831.691 kb on + strand, within Pf1N1B4_4374at 4831.691 kb on + strand, within Pf1N1B4_4374at 4831.691 kb on + strand, within Pf1N1B4_4374at 4831.691 kb on + strand, within Pf1N1B4_4374at 4831.691 kb on + strand, within Pf1N1B4_4374at 4831.692 kb on - strand, within Pf1N1B4_4374at 4831.730 kb on + strandat 4831.730 kb on + strandat 4831.730 kb on + strandat 4831.730 kb on + strandat 4831.731 kb on - strandat 4832.131 kb on - strandat 4832.262 kb on + strand, within Pf1N1B4_4375at 4832.262 kb on + strand, within Pf1N1B4_4375at 4832.263 kb on - strand, within Pf1N1B4_4375at 4832.263 kb on - strand, within Pf1N1B4_4375at 4832.263 kb on - strand, within Pf1N1B4_4375at 4832.263 kb on - strandat 4832.312 kb on - strand, within Pf1N1B4_4375at 4832.423 kb on - strandat 4832.550 kb on + strand, within Pf1N1B4_4375at 4832.763 kb on + strand, within Pf1N1B4_4375at 4832.763 kb on + strand, within Pf1N1B4_4375at 4832.763 kb on + strand, within Pf1N1B4_4375at 4832.763 kb on + strand, within Pf1N1B4_4375at 4832.764 kb on - strand, within Pf1N1B4_4375at 4832.764 kb on - strandat 4832.764 kb on - strand, within Pf1N1B4_4375at 4832.764 kb on - strand, within Pf1N1B4_4375at 4832.764 kb on - strand, within Pf1N1B4_4375at 4832.862 kb on + strand, within Pf1N1B4_4375at 4832.862 kb on + strand, within Pf1N1B4_4375at 4832.863 kb on - strand, within Pf1N1B4_4375at 4832.907 kb on + strand, within Pf1N1B4_4375at 4832.907 kb on + strand, within Pf1N1B4_4375at 4833.174 kb on + strandat 4833.402 kb on + strandat 4833.402 kb on + strand, within Pf1N1B4_4376at 4833.402 kb on + strand, within Pf1N1B4_4376at 4833.406 kb on + strand, within Pf1N1B4_4376at 4833.406 kb on + strand, within Pf1N1B4_4376at 4833.406 kb on + strandat 4833.407 kb on - strand, within Pf1N1B4_4376at 4833.407 kb on - strand, within Pf1N1B4_4376at 4833.407 kb on - strand, within Pf1N1B4_4376at 4833.407 kb on - strand, within Pf1N1B4_4376at 4834.081 kb on + strandat 4834.082 kb on - strandat 4834.082 kb on - strandat 4834.082 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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4,830,775 + +0.9
4,830,775 + Pf1N1B4_4373 0.10 -0.7
4,830,775 + Pf1N1B4_4373 0.10 -0.7
4,830,901 + +0.5
4,830,902 - -1.4
4,831,020 + +0.2
4,831,020 + +0.5
4,831,070 + -0.9
4,831,070 + +0.9
4,831,071 - -0.7
4,831,119 + -0.7
4,831,269 + +0.9
4,831,269 + -1.3
4,831,269 + +0.9
4,831,270 - +3.1
4,831,569 + Pf1N1B4_4374 0.58 +0.4
4,831,570 - Pf1N1B4_4374 0.58 -1.1
4,831,679 - Pf1N1B4_4374 0.85 -1.4
4,831,679 - Pf1N1B4_4374 0.85 -2.1
4,831,691 + Pf1N1B4_4374 0.88 +0.7
4,831,691 + Pf1N1B4_4374 0.88 -0.7
4,831,691 + Pf1N1B4_4374 0.88 -0.7
4,831,691 + Pf1N1B4_4374 0.88 +0.2
4,831,691 + Pf1N1B4_4374 0.88 -0.1
4,831,692 - Pf1N1B4_4374 0.89 +0.7
4,831,730 + -0.7
4,831,730 + +1.4
4,831,730 + +1.7
4,831,730 + -0.1
4,831,731 - -1.7
4,832,131 - +0.9
4,832,262 + Pf1N1B4_4375 0.38 +2.2
4,832,262 + Pf1N1B4_4375 0.38 +1.3
4,832,263 - Pf1N1B4_4375 0.38 -0.6
4,832,263 - Pf1N1B4_4375 0.38 +0.3
4,832,263 - Pf1N1B4_4375 0.38 +0.2
4,832,263 - -0.1
4,832,312 - Pf1N1B4_4375 0.41 +0.4
4,832,423 - +0.5
4,832,550 + Pf1N1B4_4375 0.59 +0.5
4,832,763 + Pf1N1B4_4375 0.74 +0.7
4,832,763 + Pf1N1B4_4375 0.74 +0.5
4,832,763 + Pf1N1B4_4375 0.74 -2.8
4,832,763 + Pf1N1B4_4375 0.74 -1.9
4,832,764 - Pf1N1B4_4375 0.75 +0.5
4,832,764 - -0.1
4,832,764 - Pf1N1B4_4375 0.75 -1.4
4,832,764 - Pf1N1B4_4375 0.75 +0.5
4,832,764 - Pf1N1B4_4375 0.75 +2.8
4,832,862 + Pf1N1B4_4375 0.82 -0.4
4,832,862 + Pf1N1B4_4375 0.82 +0.5
4,832,863 - Pf1N1B4_4375 0.82 +1.9
4,832,907 + Pf1N1B4_4375 0.85 -1.1
4,832,907 + Pf1N1B4_4375 0.85 -1.7
4,833,174 + +2.9
4,833,402 + +1.2
4,833,402 + Pf1N1B4_4376 0.30 +0.3
4,833,402 + Pf1N1B4_4376 0.30 +0.1
4,833,406 + Pf1N1B4_4376 0.31 -0.7
4,833,406 + Pf1N1B4_4376 0.31 -1.7
4,833,406 + -0.7
4,833,407 - Pf1N1B4_4376 0.31 -1.7
4,833,407 - Pf1N1B4_4376 0.31 +1.0
4,833,407 - Pf1N1B4_4376 0.31 -2.9
4,833,407 - Pf1N1B4_4376 0.31 -1.9
4,834,081 + -0.7
4,834,082 - -0.4
4,834,082 - -0.1
4,834,082 - -1.4

Or see this region's nucleotide sequence