Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt
Pf1N1B4_431 - hypothetical protein, at 504,126 to 505,139
_431
Position (kb) 504
505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 503.150 kb on + strand at 503.150 kb on + strand at 503.226 kb on - strand at 503.272 kb on - strand at 503.290 kb on + strand at 503.291 kb on - strand at 503.349 kb on + strand at 503.351 kb on + strand at 503.351 kb on + strand at 503.351 kb on + strand at 503.352 kb on - strand at 503.352 kb on - strand at 503.379 kb on + strand at 503.379 kb on + strand at 503.380 kb on - strand at 503.380 kb on - strand at 503.380 kb on - strand at 503.448 kb on + strand at 503.448 kb on + strand at 503.449 kb on - strand at 503.453 kb on + strand at 503.472 kb on + strand at 503.472 kb on + strand at 503.474 kb on + strand at 503.474 kb on + strand at 503.474 kb on + strand at 503.474 kb on + strand at 503.605 kb on + strand at 503.605 kb on + strand at 503.605 kb on + strand at 503.606 kb on - strand at 503.606 kb on - strand at 503.606 kb on - strand at 503.607 kb on - strand at 503.661 kb on + strand at 503.694 kb on - strand at 503.841 kb on - strand at 503.841 kb on - strand at 503.841 kb on - strand at 503.851 kb on - strand at 503.881 kb on - strand at 503.920 kb on + strand at 503.920 kb on + strand at 503.920 kb on + strand at 503.921 kb on - strand at 503.921 kb on - strand at 503.921 kb on - strand at 503.921 kb on - strand at 503.921 kb on - strand at 503.994 kb on + strand at 503.994 kb on + strand at 503.994 kb on + strand at 503.994 kb on + strand at 503.994 kb on + strand at 503.995 kb on - strand at 503.995 kb on - strand at 503.995 kb on - strand at 503.995 kb on - strand at 503.995 kb on - strand at 503.995 kb on - strand at 504.000 kb on + strand at 504.127 kb on + strand at 504.128 kb on - strand at 504.128 kb on - strand at 504.178 kb on + strand at 504.225 kb on + strand at 504.226 kb on - strand at 504.262 kb on - strand, within Pf1N1B4_431 at 504.288 kb on - strand, within Pf1N1B4_431 at 504.448 kb on - strand, within Pf1N1B4_431 at 504.460 kb on - strand, within Pf1N1B4_431 at 504.527 kb on - strand, within Pf1N1B4_431 at 504.723 kb on - strand, within Pf1N1B4_431 at 504.728 kb on - strand, within Pf1N1B4_431 at 504.818 kb on + strand, within Pf1N1B4_431 at 504.823 kb on + strand, within Pf1N1B4_431 at 504.855 kb on + strand, within Pf1N1B4_431 at 504.887 kb on - strand, within Pf1N1B4_431 at 504.911 kb on + strand, within Pf1N1B4_431 at 504.951 kb on + strand, within Pf1N1B4_431 at 504.951 kb on + strand, within Pf1N1B4_431 at 504.951 kb on + strand, within Pf1N1B4_431 at 504.952 kb on - strand, within Pf1N1B4_431 at 504.955 kb on + strand, within Pf1N1B4_431 at 505.053 kb on - strand at 505.177 kb on - strand at 505.184 kb on - strand at 505.251 kb on - strand at 505.268 kb on + strand at 505.269 kb on - strand at 505.272 kb on + strand at 505.282 kb on + strand at 505.282 kb on + strand at 505.282 kb on + strand at 505.283 kb on - strand at 505.283 kb on - strand at 505.283 kb on - strand at 505.283 kb on - strand at 505.613 kb on - strand at 505.613 kb on - strand at 505.680 kb on + strand at 505.681 kb on - strand at 505.738 kb on + strand at 505.739 kb on - strand at 505.751 kb on - strand at 505.804 kb on + strand at 505.895 kb on + strand at 505.896 kb on - strand at 505.896 kb on - strand at 505.896 kb on - strand at 505.956 kb on + strand at 505.956 kb on + strand at 505.957 kb on - strand at 505.957 kb on - strand at 506.012 kb on + strand at 506.012 kb on + strand at 506.059 kb on + strand at 506.059 kb on + strand at 506.060 kb on - strand at 506.061 kb on + strand at 506.061 kb on + strand at 506.061 kb on + strand at 506.061 kb on + strand at 506.062 kb on - strand at 506.095 kb on + strand at 506.095 kb on + strand at 506.095 kb on + strand at 506.095 kb on + strand at 506.095 kb on + strand at 506.096 kb on - strand at 506.096 kb on - strand at 506.101 kb on + strand at 506.101 kb on + strand at 506.139 kb on + strand at 506.139 kb on + strand at 506.139 kb on + strand at 506.139 kb on + strand at 506.139 kb on + strand at 506.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 503,150 + -1.7 503,150 + +0.3 503,226 - +1.4 503,272 - +1.9 503,290 + +2.6 503,291 - +1.1 503,349 + -0.4 503,351 + -2.0 503,351 + -1.0 503,351 + -0.1 503,352 - +0.8 503,352 - +0.4 503,379 + -0.9 503,379 + -0.5 503,380 - -0.4 503,380 - +1.1 503,380 - -1.3 503,448 + +0.6 503,448 + -0.9 503,449 - +0.9 503,453 + -0.9 503,472 + -2.3 503,472 + -0.3 503,474 + -3.1 503,474 + +1.4 503,474 + -2.4 503,474 + +0.1 503,605 + +0.3 503,605 + -1.4 503,605 + -2.6 503,606 - +2.0 503,606 - +0.9 503,606 - +0.4 503,607 - +1.1 503,661 + -0.3 503,694 - +1.1 503,841 - +0.5 503,841 - -2.0 503,841 - -0.3 503,851 - -0.2 503,881 - +3.5 503,920 + -0.4 503,920 + +1.7 503,920 + -1.9 503,921 - -1.7 503,921 - -0.0 503,921 - -1.2 503,921 - -0.9 503,921 - -2.0 503,994 + -0.4 503,994 + -1.2 503,994 + -1.4 503,994 + -0.1 503,994 + -1.0 503,995 - -0.2 503,995 - -1.4 503,995 - -1.3 503,995 - +0.1 503,995 - -0.0 503,995 - -0.9 504,000 + +2.0 504,127 + -1.7 504,128 - +0.5 504,128 - -0.4 504,178 + +0.7 504,225 + +0.2 504,226 - -0.4 504,262 - Pf1N1B4_431 0.13 -0.6 504,288 - Pf1N1B4_431 0.16 +1.1 504,448 - Pf1N1B4_431 0.32 +0.1 504,460 - Pf1N1B4_431 0.33 +0.1 504,527 - Pf1N1B4_431 0.40 -1.4 504,723 - Pf1N1B4_431 0.59 -0.1 504,728 - Pf1N1B4_431 0.59 -0.7 504,818 + Pf1N1B4_431 0.68 -2.0 504,823 + Pf1N1B4_431 0.69 -1.6 504,855 + Pf1N1B4_431 0.72 -2.3 504,887 - Pf1N1B4_431 0.75 -0.9 504,911 + Pf1N1B4_431 0.77 +0.1 504,951 + Pf1N1B4_431 0.81 +0.6 504,951 + Pf1N1B4_431 0.81 -0.9 504,951 + Pf1N1B4_431 0.81 -2.0 504,952 - Pf1N1B4_431 0.81 -1.2 504,955 + Pf1N1B4_431 0.82 -1.5 505,053 - +1.1 505,177 - -1.2 505,184 - -1.8 505,251 - -1.2 505,268 + -0.4 505,269 - +0.1 505,272 + -1.2 505,282 + -1.2 505,282 + -1.4 505,282 + -0.1 505,283 - -0.6 505,283 - -0.2 505,283 - -2.7 505,283 - +0.6 505,613 - -0.3 505,613 - -0.4 505,680 + -0.4 505,681 - -1.1 505,738 + -1.2 505,739 - +0.1 505,751 - -0.4 505,804 + -0.4 505,895 + +1.1 505,896 - +1.0 505,896 - -0.9 505,896 - +0.3 505,956 + -0.7 505,956 + -1.2 505,957 - +0.3 505,957 - -0.9 506,012 + -0.9 506,012 + -1.7 506,059 + +0.1 506,059 + -0.4 506,060 - -0.7 506,061 + +1.1 506,061 + +0.1 506,061 + -0.2 506,061 + -0.2 506,062 - +1.4 506,095 + +0.6 506,095 + -1.9 506,095 + +0.1 506,095 + -1.4 506,095 + +0.7 506,096 - -1.4 506,096 - -0.2 506,101 + +0.6 506,101 + -0.9 506,139 + +0.7 506,139 + -0.4 506,139 + +0.9 506,139 + +0.1 506,139 + -1.2 506,139 + -1.3
Or see this region's nucleotide sequence