Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4300 and Pf1N1B4_4301 are separated by 487 nucleotides Pf1N1B4_4301 and Pf1N1B4_4302 are separated by 48 nucleotides Pf1N1B4_4302 and Pf1N1B4_4303 are separated by 170 nucleotides
Pf1N1B4_4300 - Transcriptional regulatory protein ompR, at 4,763,970 to 4,764,710
_4300
Pf1N1B4_4301 - hypothetical protein, at 4,765,198 to 4,765,461
_4301
Pf1N1B4_4302 - hypothetical protein, at 4,765,510 to 4,765,731
_4302
Pf1N1B4_4303 - DipZ protein, at 4,765,902 to 4,767,113
_4303
Position (kb)
4765
4766 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4764.404 kb on - strand, within Pf1N1B4_4300 at 4764.784 kb on + strand at 4764.785 kb on - strand at 4764.785 kb on - strand at 4764.794 kb on + strand at 4764.794 kb on + strand at 4764.814 kb on - strand at 4764.861 kb on - strand at 4764.861 kb on - strand at 4765.034 kb on + strand at 4765.174 kb on + strand at 4765.175 kb on - strand at 4765.175 kb on - strand at 4765.175 kb on - strand at 4765.294 kb on + strand, within Pf1N1B4_4301 at 4765.294 kb on + strand, within Pf1N1B4_4301 at 4765.295 kb on - strand, within Pf1N1B4_4301 at 4765.295 kb on - strand, within Pf1N1B4_4301 at 4765.301 kb on - strand, within Pf1N1B4_4301 at 4765.387 kb on + strand, within Pf1N1B4_4301 at 4765.388 kb on - strand, within Pf1N1B4_4301 at 4765.423 kb on + strand, within Pf1N1B4_4301 at 4765.423 kb on + strand, within Pf1N1B4_4301 at 4765.424 kb on - strand, within Pf1N1B4_4301 at 4765.721 kb on + strand at 4765.721 kb on + strand at 4765.884 kb on - strand at 4765.884 kb on - strand at 4765.884 kb on - strand at 4766.374 kb on - strand, within Pf1N1B4_4303
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,764,404 - Pf1N1B4_4300 0.59 +1.1 4,764,784 + -2.1 4,764,785 - -2.7 4,764,785 - -0.4 4,764,794 + +3.2 4,764,794 + -1.3 4,764,814 - -0.4 4,764,861 - +0.6 4,764,861 - -0.1 4,765,034 + +0.9 4,765,174 + -0.6 4,765,175 - -0.5 4,765,175 - -0.8 4,765,175 - -2.3 4,765,294 + Pf1N1B4_4301 0.36 -1.8 4,765,294 + Pf1N1B4_4301 0.36 +0.6 4,765,295 - Pf1N1B4_4301 0.37 -1.9 4,765,295 - Pf1N1B4_4301 0.37 -0.7 4,765,301 - Pf1N1B4_4301 0.39 -1.7 4,765,387 + Pf1N1B4_4301 0.72 -1.4 4,765,388 - Pf1N1B4_4301 0.72 -1.6 4,765,423 + Pf1N1B4_4301 0.85 -1.1 4,765,423 + Pf1N1B4_4301 0.85 -1.1 4,765,424 - Pf1N1B4_4301 0.86 +0.9 4,765,721 + -2.3 4,765,721 + +0.1 4,765,884 - -1.7 4,765,884 - -1.9 4,765,884 - -2.1 4,766,374 - Pf1N1B4_4303 0.39 +1.5
Or see this region's nucleotide sequence